Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23075 and BWI76_RS23080 overlap by 4 nucleotides BWI76_RS23080 and BWI76_RS23085 are separated by 10 nucleotides BWI76_RS23085 and BWI76_RS23090 overlap by 4 nucleotides
BWI76_RS23075: BWI76_RS23075 - cobalt-precorrin-5B (C(1))-methyltransferase, at 4,810,516 to 4,811,655
_RS23075
BWI76_RS23080: BWI76_RS23080 - precorrin-8X methylmutase, at 4,811,652 to 4,812,284
_RS23080
BWI76_RS23085: BWI76_RS23085 - adenosylcobinamide-phosphate synthase, at 4,812,295 to 4,813,254
_RS23085
BWI76_RS23090: BWI76_RS23090 - cobyrinic acid a,c-diamide synthase, at 4,813,251 to 4,814,630
_RS23090
Position (kb)
4812
4813
4814 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4811.304 kb on - strand, within BWI76_RS23075 at 4811.461 kb on - strand at 4811.952 kb on - strand, within BWI76_RS23080 at 4812.051 kb on + strand, within BWI76_RS23080 at 4812.082 kb on + strand, within BWI76_RS23080 at 4812.259 kb on + strand at 4812.326 kb on + strand at 4812.334 kb on - strand at 4812.334 kb on - strand at 4812.334 kb on - strand at 4812.358 kb on - strand at 4812.521 kb on + strand, within BWI76_RS23085 at 4812.525 kb on - strand, within BWI76_RS23085 at 4812.581 kb on + strand, within BWI76_RS23085 at 4812.589 kb on - strand, within BWI76_RS23085 at 4812.601 kb on + strand, within BWI76_RS23085 at 4812.616 kb on + strand, within BWI76_RS23085 at 4812.624 kb on - strand, within BWI76_RS23085 at 4812.637 kb on - strand, within BWI76_RS23085 at 4812.712 kb on + strand, within BWI76_RS23085 at 4812.825 kb on - strand, within BWI76_RS23085 at 4812.967 kb on - strand, within BWI76_RS23085 at 4813.365 kb on + strand at 4813.375 kb on - strand at 4813.524 kb on + strand, within BWI76_RS23090 at 4813.595 kb on + strand, within BWI76_RS23090 at 4813.667 kb on - strand, within BWI76_RS23090 at 4813.757 kb on + strand, within BWI76_RS23090 at 4813.827 kb on - strand, within BWI76_RS23090 at 4813.868 kb on + strand, within BWI76_RS23090 at 4814.066 kb on + strand, within BWI76_RS23090 at 4814.087 kb on + strand, within BWI76_RS23090 at 4814.097 kb on - strand, within BWI76_RS23090 at 4814.198 kb on - strand, within BWI76_RS23090 at 4814.210 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 4,811,304 - BWI76_RS23075 0.69 -1.4 4,811,461 - -1.1 4,811,952 - BWI76_RS23080 0.47 -1.2 4,812,051 + BWI76_RS23080 0.63 -0.1 4,812,082 + BWI76_RS23080 0.68 -1.0 4,812,259 + -1.1 4,812,326 + -0.2 4,812,334 - -0.4 4,812,334 - -0.2 4,812,334 - -1.0 4,812,358 - -0.1 4,812,521 + BWI76_RS23085 0.24 +1.2 4,812,525 - BWI76_RS23085 0.24 +0.7 4,812,581 + BWI76_RS23085 0.30 -2.9 4,812,589 - BWI76_RS23085 0.31 -0.1 4,812,601 + BWI76_RS23085 0.32 +0.4 4,812,616 + BWI76_RS23085 0.33 -2.6 4,812,624 - BWI76_RS23085 0.34 +0.9 4,812,637 - BWI76_RS23085 0.36 +1.9 4,812,712 + BWI76_RS23085 0.43 +1.0 4,812,825 - BWI76_RS23085 0.55 +0.9 4,812,967 - BWI76_RS23085 0.70 +1.0 4,813,365 + -0.4 4,813,375 - +1.6 4,813,524 + BWI76_RS23090 0.20 -2.7 4,813,595 + BWI76_RS23090 0.25 +0.8 4,813,667 - BWI76_RS23090 0.30 +0.2 4,813,757 + BWI76_RS23090 0.37 -0.6 4,813,827 - BWI76_RS23090 0.42 +0.4 4,813,868 + BWI76_RS23090 0.45 -1.1 4,814,066 + BWI76_RS23090 0.59 -2.3 4,814,087 + BWI76_RS23090 0.61 +1.0 4,814,097 - BWI76_RS23090 0.61 -0.5 4,814,198 - BWI76_RS23090 0.69 +0.3 4,814,210 + -0.1
Or see this region's nucleotide sequence