Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22935 and BWI76_RS22940 are separated by 57 nucleotides BWI76_RS22940 and BWI76_RS22945 are separated by 61 nucleotides BWI76_RS22945 and BWI76_RS22950 overlap by 8 nucleotides BWI76_RS22950 and BWI76_RS22955 are separated by 19 nucleotides
BWI76_RS22935: BWI76_RS22935 - L-fucose mutarotase, at 4,789,961 to 4,790,383
_RS22935
BWI76_RS22940: BWI76_RS22940 - DNA-binding transcriptional activator FucR, at 4,790,441 to 4,791,151
_RS22940
BWI76_RS22945: BWI76_RS22945 - 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM, at 4,791,213 to 4,792,313
_RS22945
BWI76_RS22950: BWI76_RS22950 - membrane protein, at 4,792,306 to 4,792,701
_RS22950
BWI76_RS22955: BWI76_RS22955 - transcriptional regulator GcvA, at 4,792,721 to 4,793,638
_RS22955
Position (kb)
4791
4792
4793 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4790.311 kb on - strand, within BWI76_RS22935 at 4790.564 kb on + strand, within BWI76_RS22940 at 4790.920 kb on + strand, within BWI76_RS22940 at 4790.971 kb on - strand, within BWI76_RS22940 at 4791.258 kb on + strand at 4791.324 kb on - strand, within BWI76_RS22945 at 4791.491 kb on + strand, within BWI76_RS22945 at 4791.502 kb on + strand, within BWI76_RS22945 at 4791.636 kb on + strand, within BWI76_RS22945 at 4791.660 kb on + strand, within BWI76_RS22945 at 4792.024 kb on + strand, within BWI76_RS22945 at 4792.229 kb on + strand at 4792.393 kb on - strand, within BWI76_RS22950 at 4792.436 kb on - strand, within BWI76_RS22950 at 4792.704 kb on - strand at 4792.705 kb on - strand at 4792.705 kb on - strand at 4792.805 kb on - strand at 4793.060 kb on + strand, within BWI76_RS22955
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 4,790,311 - BWI76_RS22935 0.83 +1.4 4,790,564 + BWI76_RS22940 0.17 -2.5 4,790,920 + BWI76_RS22940 0.67 -0.2 4,790,971 - BWI76_RS22940 0.75 -2.8 4,791,258 + +0.4 4,791,324 - BWI76_RS22945 0.10 +0.8 4,791,491 + BWI76_RS22945 0.25 +0.5 4,791,502 + BWI76_RS22945 0.26 +0.8 4,791,636 + BWI76_RS22945 0.38 +1.6 4,791,660 + BWI76_RS22945 0.41 +2.7 4,792,024 + BWI76_RS22945 0.74 -0.7 4,792,229 + +0.1 4,792,393 - BWI76_RS22950 0.22 +0.6 4,792,436 - BWI76_RS22950 0.33 -0.7 4,792,704 - -1.4 4,792,705 - +0.6 4,792,705 - -0.1 4,792,805 - +1.1 4,793,060 + BWI76_RS22955 0.37 -2.8
Or see this region's nucleotide sequence