Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20995 and BWI76_RS21000 are separated by 3 nucleotides BWI76_RS21000 and BWI76_RS21005 are separated by 37 nucleotides
BWI76_RS20995: BWI76_RS20995 - RNA degradosome polyphosphate kinase, at 4,390,251 to 4,392,311
_RS20995
BWI76_RS21000: BWI76_RS21000 - exopolyphosphatase, at 4,392,315 to 4,393,856
_RS21000
BWI76_RS21005: BWI76_RS21005 - sensor domain-containing phosphodiesterase, at 4,393,894 to 4,396,116
_RS21005
Position (kb)
4392
4393
4394 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4391.642 kb on + strand, within BWI76_RS20995 at 4392.555 kb on - strand, within BWI76_RS21000 at 4392.635 kb on + strand, within BWI76_RS21000 at 4393.187 kb on - strand, within BWI76_RS21000 at 4393.289 kb on + strand, within BWI76_RS21000 at 4393.289 kb on + strand, within BWI76_RS21000 at 4393.367 kb on + strand, within BWI76_RS21000 at 4393.395 kb on + strand, within BWI76_RS21000 at 4393.662 kb on - strand, within BWI76_RS21000 at 4393.692 kb on - strand, within BWI76_RS21000 at 4393.716 kb on + strand at 4393.832 kb on + strand at 4393.878 kb on - strand at 4393.947 kb on - strand at 4393.948 kb on - strand at 4393.953 kb on - strand at 4393.964 kb on - strand at 4394.078 kb on + strand at 4394.079 kb on - strand at 4394.195 kb on - strand, within BWI76_RS21005 at 4394.460 kb on + strand, within BWI76_RS21005 at 4394.568 kb on - strand, within BWI76_RS21005 at 4394.572 kb on + strand, within BWI76_RS21005 at 4394.736 kb on - strand, within BWI76_RS21005 at 4394.799 kb on - strand, within BWI76_RS21005 at 4394.799 kb on - strand, within BWI76_RS21005 at 4394.810 kb on + strand, within BWI76_RS21005
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 4,391,642 + BWI76_RS20995 0.67 +1.8 4,392,555 - BWI76_RS21000 0.16 -0.9 4,392,635 + BWI76_RS21000 0.21 -1.3 4,393,187 - BWI76_RS21000 0.57 +0.3 4,393,289 + BWI76_RS21000 0.63 -1.2 4,393,289 + BWI76_RS21000 0.63 -1.2 4,393,367 + BWI76_RS21000 0.68 -3.4 4,393,395 + BWI76_RS21000 0.70 -0.9 4,393,662 - BWI76_RS21000 0.87 -2.1 4,393,692 - BWI76_RS21000 0.89 +0.7 4,393,716 + -1.1 4,393,832 + +0.1 4,393,878 - +0.9 4,393,947 - -0.2 4,393,948 - -0.6 4,393,953 - +2.9 4,393,964 - -0.9 4,394,078 + -0.6 4,394,079 - -0.3 4,394,195 - BWI76_RS21005 0.14 +0.7 4,394,460 + BWI76_RS21005 0.25 -1.9 4,394,568 - BWI76_RS21005 0.30 -1.4 4,394,572 + BWI76_RS21005 0.30 +0.8 4,394,736 - BWI76_RS21005 0.38 +2.1 4,394,799 - BWI76_RS21005 0.41 +1.2 4,394,799 - BWI76_RS21005 0.41 +0.6 4,394,810 + BWI76_RS21005 0.41 +0.5
Or see this region's nucleotide sequence