Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20925 and BWI76_RS20930 are separated by 9 nucleotides BWI76_RS20930 and BWI76_RS20935 are separated by 28 nucleotides BWI76_RS20935 and BWI76_RS20940 are separated by 71 nucleotides
BWI76_RS20925: BWI76_RS20925 - peptidase M48, at 4,374,919 to 4,376,382
_RS20925
BWI76_RS20930: BWI76_RS20930 - arsenate reductase (glutaredoxin), at 4,376,392 to 4,376,751
_RS20930
BWI76_RS20935: BWI76_RS20935 - DnaA regulatory inactivator Hda, at 4,376,780 to 4,377,505
_RS20935
BWI76_RS20940: BWI76_RS20940 - uracil/xanthine transporter, at 4,377,577 to 4,378,866
_RS20940
Position (kb)
4376
4377 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4375.658 kb on - strand, within BWI76_RS20925 at 4375.849 kb on + strand, within BWI76_RS20925 at 4375.860 kb on - strand, within BWI76_RS20925 at 4375.877 kb on - strand, within BWI76_RS20925 at 4376.118 kb on + strand, within BWI76_RS20925 at 4376.308 kb on - strand at 4376.437 kb on - strand, within BWI76_RS20930 at 4376.447 kb on + strand, within BWI76_RS20930 at 4376.761 kb on + strand at 4377.517 kb on + strand at 4377.548 kb on - strand at 4377.568 kb on + strand at 4377.576 kb on - strand at 4377.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 4,375,658 - BWI76_RS20925 0.50 +0.1 4,375,849 + BWI76_RS20925 0.64 -0.5 4,375,860 - BWI76_RS20925 0.64 -0.4 4,375,877 - BWI76_RS20925 0.65 -2.9 4,376,118 + BWI76_RS20925 0.82 +0.5 4,376,308 - -0.5 4,376,437 - BWI76_RS20930 0.12 -1.4 4,376,447 + BWI76_RS20930 0.15 -2.1 4,376,761 + -1.3 4,377,517 + +1.9 4,377,548 - +0.3 4,377,568 + -0.1 4,377,576 - +0.4 4,377,703 - +0.0
Or see this region's nucleotide sequence