Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20835 and BWI76_RS20840 overlap by 32 nucleotides BWI76_RS20840 and BWI76_RS20845 are separated by 78 nucleotides BWI76_RS20845 and BWI76_RS20850 are separated by 58 nucleotides
BWI76_RS20835: BWI76_RS20835 - transketolase, at 4,354,083 to 4,356,077
_RS20835
BWI76_RS20840: BWI76_RS20840 - hypothetical protein, at 4,356,046 to 4,357,113
_RS20840
BWI76_RS20845: BWI76_RS20845 - GDP-mannose pyrophosphatase NudK, at 4,357,192 to 4,357,776
_RS20845
BWI76_RS20850: BWI76_RS20850 - oxidoreductase FeS-binding subunit, at 4,357,835 to 4,359,799
_RS20850
Position (kb)
4356
4357
4358 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4355.134 kb on - strand, within BWI76_RS20835 at 4355.239 kb on - strand, within BWI76_RS20835 at 4355.378 kb on - strand, within BWI76_RS20835 at 4355.450 kb on + strand, within BWI76_RS20835 at 4355.603 kb on + strand, within BWI76_RS20835 at 4355.803 kb on - strand, within BWI76_RS20835 at 4355.803 kb on - strand, within BWI76_RS20835 at 4355.858 kb on - strand, within BWI76_RS20835 at 4356.071 kb on - strand at 4356.080 kb on - strand at 4356.279 kb on + strand, within BWI76_RS20840 at 4356.306 kb on + strand, within BWI76_RS20840 at 4356.337 kb on - strand, within BWI76_RS20840 at 4356.674 kb on + strand, within BWI76_RS20840 at 4356.719 kb on + strand, within BWI76_RS20840 at 4356.742 kb on - strand, within BWI76_RS20840 at 4356.930 kb on + strand, within BWI76_RS20840 at 4357.582 kb on - strand, within BWI76_RS20845 at 4357.656 kb on - strand, within BWI76_RS20845 at 4357.656 kb on - strand, within BWI76_RS20845 at 4357.807 kb on + strand at 4357.834 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 4,355,134 - BWI76_RS20835 0.53 +1.1 4,355,239 - BWI76_RS20835 0.58 +0.8 4,355,378 - BWI76_RS20835 0.65 +0.0 4,355,450 + BWI76_RS20835 0.69 -1.7 4,355,603 + BWI76_RS20835 0.76 -1.1 4,355,803 - BWI76_RS20835 0.86 +1.2 4,355,803 - BWI76_RS20835 0.86 +0.8 4,355,858 - BWI76_RS20835 0.89 +0.3 4,356,071 - +0.3 4,356,080 - +1.1 4,356,279 + BWI76_RS20840 0.22 -0.4 4,356,306 + BWI76_RS20840 0.24 +1.1 4,356,337 - BWI76_RS20840 0.27 +1.1 4,356,674 + BWI76_RS20840 0.59 +0.4 4,356,719 + BWI76_RS20840 0.63 +1.2 4,356,742 - BWI76_RS20840 0.65 +2.6 4,356,930 + BWI76_RS20840 0.83 +0.3 4,357,582 - BWI76_RS20845 0.67 -0.3 4,357,656 - BWI76_RS20845 0.79 -1.4 4,357,656 - BWI76_RS20845 0.79 -1.4 4,357,807 + +3.4 4,357,834 - +0.6
Or see this region's nucleotide sequence