Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20515 and BWI76_RS20520 overlap by 4 nucleotides BWI76_RS20520 and BWI76_RS20525 are separated by 176 nucleotides
BWI76_RS20515: BWI76_RS20515 - ion channel protein, at 4,296,297 to 4,297,532
_RS20515
BWI76_RS20520: BWI76_RS20520 - indolepyruvate decarboxylase, at 4,297,529 to 4,299,190
_RS20520
BWI76_RS20525: BWI76_RS20525 - glyceraldehyde 3-phosphate reductase, at 4,299,367 to 4,300,359
_RS20525
Position (kb)
4297
4298
4299
4300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4296.637 kb on + strand at 4296.645 kb on - strand, within BWI76_RS20515 at 4296.779 kb on - strand, within BWI76_RS20515 at 4296.955 kb on - strand, within BWI76_RS20515 at 4297.144 kb on - strand, within BWI76_RS20515 at 4297.176 kb on - strand, within BWI76_RS20515 at 4297.311 kb on - strand, within BWI76_RS20515 at 4297.557 kb on - strand at 4297.561 kb on + strand at 4297.626 kb on - strand at 4297.842 kb on - strand, within BWI76_RS20520 at 4297.905 kb on + strand, within BWI76_RS20520 at 4298.009 kb on - strand, within BWI76_RS20520 at 4298.265 kb on - strand, within BWI76_RS20520 at 4298.302 kb on + strand, within BWI76_RS20520 at 4298.302 kb on + strand, within BWI76_RS20520 at 4298.629 kb on + strand, within BWI76_RS20520 at 4298.672 kb on - strand, within BWI76_RS20520 at 4298.885 kb on - strand, within BWI76_RS20520 at 4298.905 kb on - strand, within BWI76_RS20520 at 4298.914 kb on - strand, within BWI76_RS20520 at 4299.045 kb on - strand at 4299.245 kb on + strand at 4299.253 kb on - strand at 4299.253 kb on - strand at 4299.253 kb on - strand at 4299.386 kb on - strand at 4299.756 kb on - strand, within BWI76_RS20525 at 4299.825 kb on + strand, within BWI76_RS20525 at 4299.979 kb on - strand, within BWI76_RS20525 at 4300.019 kb on + strand, within BWI76_RS20525
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 4,296,637 + +3.1 4,296,645 - BWI76_RS20515 0.28 +0.7 4,296,779 - BWI76_RS20515 0.39 -0.9 4,296,955 - BWI76_RS20515 0.53 -0.1 4,297,144 - BWI76_RS20515 0.69 +0.1 4,297,176 - BWI76_RS20515 0.71 +0.5 4,297,311 - BWI76_RS20515 0.82 -1.7 4,297,557 - +0.6 4,297,561 + +0.1 4,297,626 - +0.4 4,297,842 - BWI76_RS20520 0.19 +0.3 4,297,905 + BWI76_RS20520 0.23 -0.1 4,298,009 - BWI76_RS20520 0.29 -0.3 4,298,265 - BWI76_RS20520 0.44 +0.4 4,298,302 + BWI76_RS20520 0.47 +0.6 4,298,302 + BWI76_RS20520 0.47 +1.1 4,298,629 + BWI76_RS20520 0.66 -1.5 4,298,672 - BWI76_RS20520 0.69 +0.3 4,298,885 - BWI76_RS20520 0.82 +1.1 4,298,905 - BWI76_RS20520 0.83 +0.1 4,298,914 - BWI76_RS20520 0.83 +0.8 4,299,045 - -1.2 4,299,245 + -1.3 4,299,253 - +0.0 4,299,253 - +0.2 4,299,253 - +1.1 4,299,386 - +1.6 4,299,756 - BWI76_RS20525 0.39 -1.0 4,299,825 + BWI76_RS20525 0.46 -3.7 4,299,979 - BWI76_RS20525 0.62 +1.5 4,300,019 + BWI76_RS20525 0.66 +1.1
Or see this region's nucleotide sequence