Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19225 and BWI76_RS19230 are separated by 0 nucleotides BWI76_RS19230 and BWI76_RS19235 overlap by 4 nucleotides BWI76_RS19235 and BWI76_RS19240 are separated by 64 nucleotides BWI76_RS19240 and BWI76_RS19245 are separated by 27 nucleotides
BWI76_RS19225: BWI76_RS19225 - AsnC family transcriptional regulator, at 4,001,216 to 4,001,671
_RS19225
BWI76_RS19230: BWI76_RS19230 - polar amino acid ABC transporter inner membrane subunit, at 4,001,672 to 4,002,397
_RS19230
BWI76_RS19235: BWI76_RS19235 - polar amino acid ABC transporter inner membrane subunit, at 4,002,394 to 4,003,086
_RS19235
BWI76_RS19240: BWI76_RS19240 - ABC transporter substrate-binding protein, at 4,003,151 to 4,003,924
_RS19240
BWI76_RS19245: BWI76_RS19245 - dimethylargininase, at 4,003,952 to 4,004,713
_RS19245
Position (kb)
4002
4003
4004 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4001.401 kb on + strand, within BWI76_RS19225 at 4001.570 kb on + strand, within BWI76_RS19225 at 4001.596 kb on - strand, within BWI76_RS19225 at 4001.845 kb on - strand at 4001.930 kb on - strand, within BWI76_RS19230 at 4002.240 kb on + strand, within BWI76_RS19230 at 4002.248 kb on - strand, within BWI76_RS19230 at 4002.408 kb on - strand at 4002.436 kb on - strand at 4002.533 kb on + strand, within BWI76_RS19235 at 4002.556 kb on + strand, within BWI76_RS19235 at 4002.588 kb on + strand, within BWI76_RS19235 at 4002.643 kb on + strand, within BWI76_RS19235 at 4002.836 kb on - strand, within BWI76_RS19235 at 4003.014 kb on + strand, within BWI76_RS19235 at 4003.060 kb on + strand at 4003.221 kb on + strand at 4003.403 kb on - strand, within BWI76_RS19240 at 4003.893 kb on - strand at 4003.973 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 4,001,401 + BWI76_RS19225 0.41 +1.6 4,001,570 + BWI76_RS19225 0.78 -0.4 4,001,596 - BWI76_RS19225 0.83 -0.4 4,001,845 - +1.1 4,001,930 - BWI76_RS19230 0.36 +0.8 4,002,240 + BWI76_RS19230 0.78 +2.1 4,002,248 - BWI76_RS19230 0.79 +0.0 4,002,408 - -0.5 4,002,436 - +0.1 4,002,533 + BWI76_RS19235 0.20 +1.2 4,002,556 + BWI76_RS19235 0.23 -3.0 4,002,588 + BWI76_RS19235 0.28 +0.2 4,002,643 + BWI76_RS19235 0.36 +3.4 4,002,836 - BWI76_RS19235 0.64 +2.1 4,003,014 + BWI76_RS19235 0.89 +0.3 4,003,060 + -1.4 4,003,221 + +1.7 4,003,403 - BWI76_RS19240 0.33 +1.2 4,003,893 - +1.5 4,003,973 + +0.9
Or see this region's nucleotide sequence