Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19115 and BWI76_RS19120 are separated by 27 nucleotides BWI76_RS19120 and BWI76_RS19125 are separated by 90 nucleotides BWI76_RS19125 and BWI76_RS19130 are separated by 114 nucleotides
BWI76_RS19115: BWI76_RS19115 - outer membrane assembly protein AsmA, at 3,973,669 to 3,975,516
_RS19115
BWI76_RS19120: BWI76_RS19120 - dCTP deaminase, at 3,975,544 to 3,976,125
_RS19120
BWI76_RS19125: BWI76_RS19125 - uridine kinase, at 3,976,216 to 3,976,857
_RS19125
BWI76_RS19130: BWI76_RS19130 - 3-methyladenine DNA glycosylase 2, at 3,976,972 to 3,977,820
_RS19130
Position (kb)
3976
3977 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3975.669 kb on + strand, within BWI76_RS19120 at 3976.696 kb on + strand, within BWI76_RS19125 at 3976.970 kb on - strand at 3977.038 kb on - strand at 3977.207 kb on - strand, within BWI76_RS19130 at 3977.207 kb on - strand, within BWI76_RS19130 at 3977.303 kb on - strand, within BWI76_RS19130 at 3977.330 kb on + strand, within BWI76_RS19130 at 3977.414 kb on - strand, within BWI76_RS19130 at 3977.460 kb on - strand, within BWI76_RS19130 at 3977.521 kb on - strand, within BWI76_RS19130 at 3977.624 kb on - strand, within BWI76_RS19130 at 3977.809 kb on - strand at 3977.854 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,975,669 + BWI76_RS19120 0.21 +0.5 3,976,696 + BWI76_RS19125 0.75 -0.5 3,976,970 - +0.4 3,977,038 - +0.2 3,977,207 - BWI76_RS19130 0.28 -2.4 3,977,207 - BWI76_RS19130 0.28 +0.8 3,977,303 - BWI76_RS19130 0.39 +1.4 3,977,330 + BWI76_RS19130 0.42 -2.3 3,977,414 - BWI76_RS19130 0.52 -1.3 3,977,460 - BWI76_RS19130 0.57 -2.9 3,977,521 - BWI76_RS19130 0.65 -1.9 3,977,624 - BWI76_RS19130 0.77 -0.8 3,977,809 - +0.5 3,977,854 + +0.2
Or see this region's nucleotide sequence