Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17510 and BWI76_RS17515 are separated by 50 nucleotides BWI76_RS17515 and BWI76_RS17520 are separated by 12 nucleotides BWI76_RS17520 and BWI76_RS17525 are separated by 48 nucleotides
BWI76_RS17510: BWI76_RS17510 - selenium metabolism hydrolase, at 3,631,161 to 3,632,372
_RS17510
BWI76_RS17515: BWI76_RS17515 - dihydropyrimidinase, at 3,632,423 to 3,633,829
_RS17515
BWI76_RS17520: BWI76_RS17520 - carbamate kinase, at 3,633,842 to 3,634,774
_RS17520
BWI76_RS17525: BWI76_RS17525 - XshC-Cox1-family protein, at 3,634,823 to 3,635,656
_RS17525
Position (kb)
3632
3633
3634 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3631.498 kb on + strand, within BWI76_RS17510 at 3631.631 kb on + strand, within BWI76_RS17510 at 3632.060 kb on + strand, within BWI76_RS17510 at 3632.070 kb on + strand, within BWI76_RS17510 at 3632.070 kb on + strand, within BWI76_RS17510 at 3632.078 kb on - strand, within BWI76_RS17510 at 3632.182 kb on + strand at 3632.354 kb on - strand at 3632.799 kb on + strand, within BWI76_RS17515 at 3632.876 kb on - strand, within BWI76_RS17515 at 3633.047 kb on - strand, within BWI76_RS17515 at 3633.173 kb on - strand at 3633.284 kb on + strand, within BWI76_RS17515 at 3633.354 kb on + strand, within BWI76_RS17515 at 3633.904 kb on + strand at 3633.904 kb on + strand at 3634.180 kb on - strand, within BWI76_RS17520 at 3634.396 kb on - strand, within BWI76_RS17520 at 3634.433 kb on + strand, within BWI76_RS17520 at 3634.472 kb on + strand, within BWI76_RS17520 at 3634.782 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,631,498 + BWI76_RS17510 0.28 -2.9 3,631,631 + BWI76_RS17510 0.39 +1.7 3,632,060 + BWI76_RS17510 0.74 -0.4 3,632,070 + BWI76_RS17510 0.75 -0.3 3,632,070 + BWI76_RS17510 0.75 -0.1 3,632,078 - BWI76_RS17510 0.76 +0.0 3,632,182 + -1.3 3,632,354 - +0.4 3,632,799 + BWI76_RS17515 0.27 -0.2 3,632,876 - BWI76_RS17515 0.32 +2.0 3,633,047 - BWI76_RS17515 0.44 -0.9 3,633,173 - +2.7 3,633,284 + BWI76_RS17515 0.61 +0.8 3,633,354 + BWI76_RS17515 0.66 +0.5 3,633,904 + +0.1 3,633,904 + -1.3 3,634,180 - BWI76_RS17520 0.36 -0.9 3,634,396 - BWI76_RS17520 0.59 +0.3 3,634,433 + BWI76_RS17520 0.63 +0.2 3,634,472 + BWI76_RS17520 0.68 +0.2 3,634,782 - +0.9
Or see this region's nucleotide sequence