Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16740 and BWI76_RS16745 are separated by 106 nucleotides BWI76_RS16745 and BWI76_RS16750 are separated by 10 nucleotides
BWI76_RS16740: BWI76_RS16740 - transcriptional regulator, at 3,467,493 to 3,468,365
_RS16740
BWI76_RS16745: BWI76_RS16745 - alpha-acetolactate decarboxylase, at 3,468,472 to 3,469,251
_RS16745
BWI76_RS16750: BWI76_RS16750 - acetolactate synthase, at 3,469,262 to 3,470,941
_RS16750
Position (kb)
3468
3469
3470 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3467.542 kb on - strand at 3467.583 kb on - strand, within BWI76_RS16740 at 3467.764 kb on - strand, within BWI76_RS16740 at 3468.038 kb on + strand, within BWI76_RS16740 at 3468.038 kb on + strand, within BWI76_RS16740 at 3468.205 kb on - strand at 3468.518 kb on - strand at 3468.524 kb on - strand at 3468.549 kb on - strand at 3468.559 kb on + strand, within BWI76_RS16745 at 3468.576 kb on - strand, within BWI76_RS16745 at 3468.576 kb on - strand, within BWI76_RS16745 at 3468.585 kb on + strand, within BWI76_RS16745 at 3468.585 kb on + strand, within BWI76_RS16745 at 3468.800 kb on + strand, within BWI76_RS16745 at 3469.030 kb on - strand, within BWI76_RS16745 at 3469.079 kb on + strand, within BWI76_RS16745 at 3469.307 kb on - strand at 3469.523 kb on - strand, within BWI76_RS16750 at 3469.701 kb on - strand, within BWI76_RS16750 at 3469.998 kb on - strand, within BWI76_RS16750
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,467,542 - -0.4 3,467,583 - BWI76_RS16740 0.10 +0.8 3,467,764 - BWI76_RS16740 0.31 -1.2 3,468,038 + BWI76_RS16740 0.62 +0.6 3,468,038 + BWI76_RS16740 0.62 +0.0 3,468,205 - -0.4 3,468,518 - +1.0 3,468,524 - +1.2 3,468,549 - -0.6 3,468,559 + BWI76_RS16745 0.11 +1.4 3,468,576 - BWI76_RS16745 0.13 +0.4 3,468,576 - BWI76_RS16745 0.13 +0.4 3,468,585 + BWI76_RS16745 0.14 +2.6 3,468,585 + BWI76_RS16745 0.14 +0.7 3,468,800 + BWI76_RS16745 0.42 +0.5 3,469,030 - BWI76_RS16745 0.72 -1.8 3,469,079 + BWI76_RS16745 0.78 +0.9 3,469,307 - -1.8 3,469,523 - BWI76_RS16750 0.16 +1.4 3,469,701 - BWI76_RS16750 0.26 -0.4 3,469,998 - BWI76_RS16750 0.44 +1.3
Or see this region's nucleotide sequence