Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14705 and BWI76_RS14710 are separated by 919 nucleotides BWI76_RS14710 and BWI76_RS14715 are separated by 49 nucleotides BWI76_RS14715 and BWI76_RS14720 overlap by 20 nucleotides
BWI76_RS14705: BWI76_RS14705 - SefA, at 3,087,495 to 3,088,040
_RS14705
BWI76_RS14710: BWI76_RS14710 - FAD-dependent oxidoreductase, at 3,088,960 to 3,090,282
_RS14710
BWI76_RS14715: BWI76_RS14715 - type I methionyl aminopeptidase, at 3,090,332 to 3,091,111
_RS14715
BWI76_RS14720: BWI76_RS14720 - hypothetical protein, at 3,091,092 to 3,091,310
_RS14720
Position (kb)
3088
3089
3090
3091 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3087.971 kb on - strand, within BWI76_RS14705 at 3087.972 kb on + strand, within BWI76_RS14705 at 3087.990 kb on + strand at 3087.997 kb on + strand at 3087.997 kb on + strand at 3087.997 kb on + strand at 3087.997 kb on - strand at 3087.997 kb on - strand at 3087.997 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.007 kb on - strand at 3088.016 kb on - strand at 3088.099 kb on - strand at 3088.110 kb on - strand at 3088.271 kb on - strand at 3088.284 kb on + strand at 3088.521 kb on + strand at 3088.529 kb on - strand at 3088.539 kb on - strand at 3088.574 kb on + strand at 3088.585 kb on + strand at 3088.590 kb on + strand at 3088.591 kb on + strand at 3088.623 kb on - strand at 3088.666 kb on + strand at 3088.666 kb on + strand at 3088.703 kb on - strand at 3088.773 kb on - strand at 3088.800 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.847 kb on + strand at 3088.855 kb on - strand at 3088.855 kb on - strand at 3088.855 kb on - strand at 3088.859 kb on + strand at 3088.859 kb on + strand at 3088.864 kb on + strand at 3088.864 kb on + strand at 3088.867 kb on - strand at 3088.867 kb on - strand at 3088.872 kb on - strand at 3088.914 kb on + strand at 3088.919 kb on + strand at 3089.023 kb on - strand at 3089.052 kb on + strand at 3089.060 kb on - strand at 3089.120 kb on + strand, within BWI76_RS14710 at 3089.141 kb on - strand, within BWI76_RS14710 at 3089.227 kb on - strand, within BWI76_RS14710 at 3089.405 kb on + strand, within BWI76_RS14710 at 3089.424 kb on + strand, within BWI76_RS14710 at 3089.836 kb on - strand, within BWI76_RS14710 at 3090.044 kb on - strand, within BWI76_RS14710 at 3090.516 kb on - strand, within BWI76_RS14715 at 3090.652 kb on - strand, within BWI76_RS14715 at 3090.693 kb on + strand, within BWI76_RS14715 at 3091.003 kb on - strand, within BWI76_RS14715 at 3091.020 kb on - strand, within BWI76_RS14715
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,087,971 - BWI76_RS14705 0.87 -1.6 3,087,972 + BWI76_RS14705 0.87 +1.2 3,087,990 + +1.2 3,087,997 + -0.4 3,087,997 + -0.2 3,087,997 + +1.2 3,087,997 - -1.8 3,087,997 - +0.2 3,087,997 - +0.8 3,088,005 - +1.2 3,088,005 - -3.8 3,088,005 - -2.7 3,088,005 - +0.2 3,088,005 - -0.6 3,088,005 - -0.4 3,088,007 - -0.4 3,088,016 - +1.2 3,088,099 - +0.6 3,088,110 - -1.5 3,088,271 - +1.2 3,088,284 + -1.8 3,088,521 + -0.8 3,088,529 - -0.4 3,088,539 - -2.9 3,088,574 + -1.2 3,088,585 + +0.5 3,088,590 + -0.8 3,088,591 + +0.2 3,088,623 - +0.2 3,088,666 + -0.3 3,088,666 + -1.4 3,088,703 - +1.0 3,088,773 - -2.3 3,088,800 + +0.3 3,088,825 + -0.5 3,088,825 + +0.6 3,088,825 + -0.6 3,088,825 + +0.6 3,088,825 + +0.3 3,088,825 + -0.0 3,088,825 + +1.2 3,088,825 + -0.4 3,088,825 + +1.4 3,088,825 + -1.2 3,088,825 + -2.8 3,088,833 - -1.4 3,088,833 - -0.6 3,088,833 - -0.2 3,088,833 - -1.1 3,088,833 - +1.2 3,088,833 - +0.0 3,088,833 - -0.4 3,088,833 - +0.9 3,088,833 - +0.0 3,088,833 - +0.8 3,088,847 + +0.6 3,088,855 - -1.1 3,088,855 - -0.3 3,088,855 - -1.9 3,088,859 + +2.6 3,088,859 + -0.7 3,088,864 + -0.3 3,088,864 + +1.1 3,088,867 - +0.3 3,088,867 - +0.0 3,088,872 - -0.3 3,088,914 + -1.9 3,088,919 + +1.6 3,089,023 - -1.6 3,089,052 + +0.4 3,089,060 - -1.8 3,089,120 + BWI76_RS14710 0.12 -0.3 3,089,141 - BWI76_RS14710 0.14 -0.3 3,089,227 - BWI76_RS14710 0.20 +0.0 3,089,405 + BWI76_RS14710 0.34 -1.4 3,089,424 + BWI76_RS14710 0.35 +0.5 3,089,836 - BWI76_RS14710 0.66 -0.3 3,090,044 - BWI76_RS14710 0.82 +2.1 3,090,516 - BWI76_RS14715 0.24 -0.2 3,090,652 - BWI76_RS14715 0.41 -0.8 3,090,693 + BWI76_RS14715 0.46 -0.8 3,091,003 - BWI76_RS14715 0.86 -1.1 3,091,020 - BWI76_RS14715 0.88 -0.7
Or see this region's nucleotide sequence