Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12580 and BWI76_RS12585 overlap by 4 nucleotides BWI76_RS12585 and BWI76_RS12590 are separated by 264 nucleotides BWI76_RS12590 and BWI76_RS12595 are separated by 99 nucleotides BWI76_RS12595 and BWI76_RS12600 are separated by 81 nucleotides
BWI76_RS12580: BWI76_RS12580 - oxidoreductase, at 2,626,711 to 2,627,430
_RS12580
BWI76_RS12585: BWI76_RS12585 - transcriptional regulator, at 2,627,427 to 2,627,861
_RS12585
BWI76_RS12590: BWI76_RS12590 - helix-turn-helix-type transcriptional regulator, at 2,628,126 to 2,628,854
_RS12590
BWI76_RS12595: BWI76_RS12595 - hypothetical protein, at 2,628,954 to 2,629,298
_RS12595
BWI76_RS12600: BWI76_RS12600 - MFS transporter, at 2,629,380 to 2,630,501
_RS12600
Position (kb)
2628
2629 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2627.336 kb on - strand, within BWI76_RS12580 at 2627.404 kb on + strand at 2627.610 kb on + strand, within BWI76_RS12585 at 2627.726 kb on + strand, within BWI76_RS12585 at 2627.801 kb on + strand, within BWI76_RS12585 at 2627.815 kb on + strand, within BWI76_RS12585 at 2627.868 kb on + strand at 2627.868 kb on + strand at 2627.868 kb on + strand at 2628.058 kb on - strand at 2628.400 kb on - strand, within BWI76_RS12590 at 2628.416 kb on - strand, within BWI76_RS12590 at 2628.484 kb on - strand, within BWI76_RS12590 at 2628.771 kb on - strand, within BWI76_RS12590 at 2628.771 kb on - strand, within BWI76_RS12590 at 2629.141 kb on - strand, within BWI76_RS12595 at 2629.198 kb on + strand, within BWI76_RS12595 at 2629.230 kb on - strand, within BWI76_RS12595 at 2629.282 kb on - strand at 2629.372 kb on + strand at 2629.380 kb on - strand at 2629.819 kb on - strand, within BWI76_RS12600
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,627,336 - BWI76_RS12580 0.87 +0.1 2,627,404 + +0.8 2,627,610 + BWI76_RS12585 0.42 +1.3 2,627,726 + BWI76_RS12585 0.69 +0.9 2,627,801 + BWI76_RS12585 0.86 +1.1 2,627,815 + BWI76_RS12585 0.89 +0.4 2,627,868 + +0.6 2,627,868 + +0.7 2,627,868 + +0.9 2,628,058 - -1.4 2,628,400 - BWI76_RS12590 0.38 -3.1 2,628,416 - BWI76_RS12590 0.40 -1.6 2,628,484 - BWI76_RS12590 0.49 -1.0 2,628,771 - BWI76_RS12590 0.88 -1.7 2,628,771 - BWI76_RS12590 0.88 -0.2 2,629,141 - BWI76_RS12595 0.54 +1.4 2,629,198 + BWI76_RS12595 0.71 +0.9 2,629,230 - BWI76_RS12595 0.80 -0.0 2,629,282 - +1.8 2,629,372 + -0.7 2,629,380 - +1.1 2,629,819 - BWI76_RS12600 0.39 -1.4
Or see this region's nucleotide sequence