Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12490 and BWI76_RS12495 are separated by 41 nucleotides BWI76_RS12495 and BWI76_RS12500 are separated by 101 nucleotides BWI76_RS12500 and BWI76_RS12505 overlap by 10 nucleotides BWI76_RS12505 and BWI76_RS12510 are separated by 188 nucleotides
BWI76_RS12490: BWI76_RS12490 - AbrB family transcriptional regulator, at 2,609,021 to 2,610,076
_RS12490
BWI76_RS12495: BWI76_RS12495 - 2-Cys peroxiredoxin, at 2,610,118 to 2,610,627
_RS12495
BWI76_RS12500: BWI76_RS12500 - dipeptide epimerase, at 2,610,729 to 2,611,694
_RS12500
BWI76_RS12505: BWI76_RS12505 - murein peptide amidase A, at 2,611,685 to 2,612,398
_RS12505
BWI76_RS12510: BWI76_RS12510 - peptide ABC transporter substrate-binding protein, at 2,612,587 to 2,614,203
_RS12510
Position (kb)
2610
2611
2612 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2609.745 kb on - strand, within BWI76_RS12490 at 2609.988 kb on - strand at 2610.154 kb on + strand at 2610.527 kb on + strand, within BWI76_RS12495 at 2611.041 kb on - strand, within BWI76_RS12500 at 2611.055 kb on + strand, within BWI76_RS12500 at 2611.079 kb on + strand, within BWI76_RS12500 at 2611.497 kb on + strand, within BWI76_RS12500 at 2611.695 kb on - strand at 2612.394 kb on + strand at 2612.401 kb on - strand at 2612.484 kb on + strand at 2612.484 kb on + strand at 2612.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,609,745 - BWI76_RS12490 0.69 +0.5 2,609,988 - -0.6 2,610,154 + +0.8 2,610,527 + BWI76_RS12495 0.80 -1.0 2,611,041 - BWI76_RS12500 0.32 -0.5 2,611,055 + BWI76_RS12500 0.34 +3.4 2,611,079 + BWI76_RS12500 0.36 +1.8 2,611,497 + BWI76_RS12500 0.80 +0.2 2,611,695 - +0.5 2,612,394 + -0.3 2,612,401 - +1.3 2,612,484 + -0.9 2,612,484 + -0.6 2,612,592 - -0.8
Or see this region's nucleotide sequence