Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12440 and BWI76_RS12445 overlap by 10 nucleotides BWI76_RS12445 and BWI76_RS12450 are separated by 167 nucleotides BWI76_RS12450 and BWI76_RS12455 are separated by 51 nucleotides
BWI76_RS12440: BWI76_RS12440 - MFS transporter, at 2,600,137 to 2,601,528
_RS12440
BWI76_RS12445: BWI76_RS12445 - phage shock protein operon transcriptional activator, at 2,601,519 to 2,602,493
_RS12445
BWI76_RS12450: BWI76_RS12450 - phage shock protein PspA, at 2,602,661 to 2,603,329
_RS12450
BWI76_RS12455: BWI76_RS12455 - envelope stress response membrane protein PspB, at 2,603,381 to 2,603,605
_RS12455
Position (kb)
2601
2602
2603 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2600.564 kb on + strand, within BWI76_RS12440 at 2600.739 kb on - strand, within BWI76_RS12440 at 2600.821 kb on + strand, within BWI76_RS12440 at 2600.980 kb on - strand, within BWI76_RS12440 at 2601.066 kb on - strand, within BWI76_RS12440 at 2601.192 kb on + strand, within BWI76_RS12440 at 2601.304 kb on - strand, within BWI76_RS12440 at 2601.352 kb on - strand, within BWI76_RS12440 at 2601.411 kb on + strand at 2601.442 kb on - strand at 2601.473 kb on + strand at 2601.655 kb on - strand, within BWI76_RS12445 at 2601.837 kb on + strand, within BWI76_RS12445 at 2601.993 kb on + strand, within BWI76_RS12445 at 2602.265 kb on + strand, within BWI76_RS12445 at 2602.423 kb on + strand at 2602.826 kb on + strand, within BWI76_RS12450 at 2603.123 kb on + strand, within BWI76_RS12450
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,600,564 + BWI76_RS12440 0.31 -2.5 2,600,739 - BWI76_RS12440 0.43 -2.9 2,600,821 + BWI76_RS12440 0.49 -2.2 2,600,980 - BWI76_RS12440 0.61 +2.4 2,601,066 - BWI76_RS12440 0.67 +1.9 2,601,192 + BWI76_RS12440 0.76 +0.1 2,601,304 - BWI76_RS12440 0.84 -0.6 2,601,352 - BWI76_RS12440 0.87 -0.0 2,601,411 + -1.3 2,601,442 - +0.4 2,601,473 + -0.6 2,601,655 - BWI76_RS12445 0.14 -0.8 2,601,837 + BWI76_RS12445 0.33 -1.0 2,601,993 + BWI76_RS12445 0.49 -0.0 2,602,265 + BWI76_RS12445 0.77 -1.1 2,602,423 + +1.0 2,602,826 + BWI76_RS12450 0.25 -0.5 2,603,123 + BWI76_RS12450 0.69 +0.6
Or see this region's nucleotide sequence