Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11795 and BWI76_RS11800 are separated by 230 nucleotides BWI76_RS11800 and BWI76_RS11805 are separated by 52 nucleotides BWI76_RS11805 and BWI76_RS11810 are separated by 313 nucleotides
BWI76_RS11795: BWI76_RS11795 - TonB-dependent siderophore receptor, at 2,480,311 to 2,482,491
_RS11795
BWI76_RS11800: BWI76_RS11800 - intracellular septation protein A, at 2,482,722 to 2,483,261
_RS11800
BWI76_RS11805: BWI76_RS11805 - hypothetical protein, at 2,483,314 to 2,484,057
_RS11805
BWI76_RS11810: BWI76_RS11810 - outer membrane protein OmpW, at 2,484,371 to 2,485,009
_RS11810
Position (kb)
2483
2484
2485 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2482.339 kb on - strand at 2483.339 kb on - strand at 2483.407 kb on - strand, within BWI76_RS11805 at 2483.509 kb on + strand, within BWI76_RS11805 at 2483.919 kb on + strand, within BWI76_RS11805 at 2483.952 kb on - strand, within BWI76_RS11805 at 2484.066 kb on - strand at 2484.120 kb on + strand at 2484.126 kb on - strand at 2484.197 kb on + strand at 2484.323 kb on + strand at 2484.521 kb on - strand, within BWI76_RS11810 at 2484.588 kb on + strand, within BWI76_RS11810 at 2484.801 kb on + strand, within BWI76_RS11810 at 2485.030 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,482,339 - -1.8 2,483,339 - -0.2 2,483,407 - BWI76_RS11805 0.12 +0.6 2,483,509 + BWI76_RS11805 0.26 +0.7 2,483,919 + BWI76_RS11805 0.81 +2.1 2,483,952 - BWI76_RS11805 0.86 -0.4 2,484,066 - -0.2 2,484,120 + +1.0 2,484,126 - +0.6 2,484,197 + +0.2 2,484,323 + +0.4 2,484,521 - BWI76_RS11810 0.23 +2.2 2,484,588 + BWI76_RS11810 0.34 +0.4 2,484,801 + BWI76_RS11810 0.67 +1.2 2,485,030 + +1.4
Or see this region's nucleotide sequence