Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10675 and BWI76_RS10680 are separated by 32 nucleotides BWI76_RS10680 and BWI76_RS10685 are separated by 103 nucleotides
BWI76_RS10675: BWI76_RS10675 - phosphoenolpyruvate--protein phosphotransferase, at 2,254,138 to 2,256,639
_RS10675
BWI76_RS10680: BWI76_RS10680 - tartrate dehydrogenase, at 2,256,672 to 2,257,790
_RS10680
BWI76_RS10685: BWI76_RS10685 - LysR family transcriptional regulator, at 2,257,894 to 2,258,850
_RS10685
Position (kb)
2256
2257
2258 Strain fitness (log2 ratio)
-1
0
1 at 2255.908 kb on - strand, within BWI76_RS10675 at 2256.226 kb on - strand, within BWI76_RS10675 at 2256.245 kb on + strand, within BWI76_RS10675 at 2256.245 kb on + strand, within BWI76_RS10675 at 2256.253 kb on - strand, within BWI76_RS10675 at 2256.299 kb on + strand, within BWI76_RS10675 at 2256.634 kb on - strand at 2257.058 kb on + strand, within BWI76_RS10680 at 2257.097 kb on + strand, within BWI76_RS10680 at 2257.667 kb on + strand, within BWI76_RS10680 at 2257.928 kb on + strand at 2257.928 kb on + strand at 2258.359 kb on - strand, within BWI76_RS10685 at 2258.458 kb on + strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.537 kb on + strand, within BWI76_RS10685
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,255,908 - BWI76_RS10675 0.71 +1.4 2,256,226 - BWI76_RS10675 0.83 -0.5 2,256,245 + BWI76_RS10675 0.84 +0.4 2,256,245 + BWI76_RS10675 0.84 -0.1 2,256,253 - BWI76_RS10675 0.85 +0.1 2,256,299 + BWI76_RS10675 0.86 +0.2 2,256,634 - +0.3 2,257,058 + BWI76_RS10680 0.34 +1.1 2,257,097 + BWI76_RS10680 0.38 +1.0 2,257,667 + BWI76_RS10680 0.89 +0.5 2,257,928 + +0.3 2,257,928 + -0.6 2,258,359 - BWI76_RS10685 0.49 +0.3 2,258,458 + BWI76_RS10685 0.59 +0.3 2,258,466 - BWI76_RS10685 0.60 -0.4 2,258,466 - BWI76_RS10685 0.60 +0.2 2,258,466 - BWI76_RS10685 0.60 +0.6 2,258,537 + BWI76_RS10685 0.67 +1.4
Or see this region's nucleotide sequence