Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10000

Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09995 and BWI76_RS10000 are separated by 28 nucleotidesBWI76_RS10000 and BWI76_RS10005 are separated by 154 nucleotides BWI76_RS09995: BWI76_RS09995 - DNA helicase IV, at 2,103,951 to 2,106,005 _RS09995 BWI76_RS10000: BWI76_RS10000 - methylglyoxal synthase, at 2,106,034 to 2,106,492 _RS10000 BWI76_RS10005: BWI76_RS10005 - CoA-binding protein, at 2,106,647 to 2,107,060 _RS10005 Position (kb) 2106 2107Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2105.437 kb on - strand, within BWI76_RS09995at 2105.444 kb on + strand, within BWI76_RS09995at 2105.444 kb on + strand, within BWI76_RS09995at 2105.534 kb on - strand, within BWI76_RS09995at 2105.879 kb on - strandat 2105.910 kb on + strandat 2105.910 kb on + strandat 2105.918 kb on - strandat 2105.918 kb on - strandat 2106.056 kb on - strandat 2106.128 kb on + strand, within BWI76_RS10000at 2106.128 kb on + strand, within BWI76_RS10000at 2106.197 kb on - strand, within BWI76_RS10000at 2106.197 kb on - strand, within BWI76_RS10000at 2106.269 kb on + strand, within BWI76_RS10000at 2106.277 kb on - strand, within BWI76_RS10000at 2106.323 kb on - strand, within BWI76_RS10000at 2106.323 kb on - strand, within BWI76_RS10000at 2106.323 kb on - strand, within BWI76_RS10000at 2106.447 kb on + strandat 2106.455 kb on - strandat 2106.524 kb on - strandat 2106.582 kb on + strandat 2106.584 kb on - strandat 2106.626 kb on - strandat 2106.708 kb on - strand, within BWI76_RS10005at 2106.708 kb on - strand, within BWI76_RS10005at 2106.720 kb on - strand, within BWI76_RS10005at 2106.772 kb on + strand, within BWI76_RS10005at 2106.801 kb on + strand, within BWI76_RS10005at 2106.804 kb on + strand, within BWI76_RS10005at 2106.817 kb on - strand, within BWI76_RS10005at 2106.882 kb on + strand, within BWI76_RS10005at 2106.905 kb on + strand, within BWI76_RS10005at 2106.949 kb on + strand, within BWI76_RS10005at 2106.985 kb on - strand, within BWI76_RS10005at 2106.998 kb on - strand, within BWI76_RS10005at 2107.007 kb on + strand, within BWI76_RS10005at 2107.042 kb on + strandat 2107.046 kb on + strandat 2107.061 kb on + strandat 2107.061 kb on + strandat 2107.090 kb on - strandat 2107.091 kb on - strandat 2107.091 kb on - strandat 2107.188 kb on - strandat 2107.196 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
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2,105,437 - BWI76_RS09995 0.72 +0.0
2,105,444 + BWI76_RS09995 0.73 -0.5
2,105,444 + BWI76_RS09995 0.73 -0.5
2,105,534 - BWI76_RS09995 0.77 -2.0
2,105,879 - -0.0
2,105,910 + +1.1
2,105,910 + -0.0
2,105,918 - +1.7
2,105,918 - -2.5
2,106,056 - -0.3
2,106,128 + BWI76_RS10000 0.20 -1.5
2,106,128 + BWI76_RS10000 0.20 -0.9
2,106,197 - BWI76_RS10000 0.36 -0.8
2,106,197 - BWI76_RS10000 0.36 -0.4
2,106,269 + BWI76_RS10000 0.51 -0.8
2,106,277 - BWI76_RS10000 0.53 -2.0
2,106,323 - BWI76_RS10000 0.63 +0.7
2,106,323 - BWI76_RS10000 0.63 -1.3
2,106,323 - BWI76_RS10000 0.63 +0.7
2,106,447 + -1.7
2,106,455 - -1.1
2,106,524 - +0.2
2,106,582 + +0.6
2,106,584 - -0.4
2,106,626 - -1.9
2,106,708 - BWI76_RS10005 0.15 +0.7
2,106,708 - BWI76_RS10005 0.15 +0.1
2,106,720 - BWI76_RS10005 0.18 +0.1
2,106,772 + BWI76_RS10005 0.30 -0.3
2,106,801 + BWI76_RS10005 0.37 -1.0
2,106,804 + BWI76_RS10005 0.38 -0.4
2,106,817 - BWI76_RS10005 0.41 -0.4
2,106,882 + BWI76_RS10005 0.57 -1.1
2,106,905 + BWI76_RS10005 0.62 -0.3
2,106,949 + BWI76_RS10005 0.73 -1.3
2,106,985 - BWI76_RS10005 0.82 +0.1
2,106,998 - BWI76_RS10005 0.85 +3.1
2,107,007 + BWI76_RS10005 0.87 +0.2
2,107,042 + +0.9
2,107,046 + -0.9
2,107,061 + +0.7
2,107,061 + -0.3
2,107,090 - -0.4
2,107,091 - -0.9
2,107,091 - -0.9
2,107,188 - +3.5
2,107,196 - +1.3

Or see this region's nucleotide sequence