Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08580 and BWI76_RS08585 are separated by 35 nucleotides BWI76_RS08585 and BWI76_RS08590 overlap by 4 nucleotides BWI76_RS08590 and BWI76_RS08595 are separated by 118 nucleotides BWI76_RS08595 and BWI76_RS08600 are separated by 315 nucleotides
BWI76_RS08580: BWI76_RS08580 - quinolinate synthetase, at 1,810,337 to 1,811,380
_RS08580
BWI76_RS08585: BWI76_RS08585 - nicotinamide riboside transporter PnuC, at 1,811,416 to 1,812,135
_RS08585
BWI76_RS08590: BWI76_RS08590 - zinc transporter ZitB, at 1,812,132 to 1,813,076
_RS08590
BWI76_RS08595: BWI76_RS08595 - hypothetical protein, at 1,813,195 to 1,813,566
_RS08595
BWI76_RS08600: BWI76_RS08600 - 3-deoxy-7-phosphoheptulonate synthase, at 1,813,882 to 1,814,934
_RS08600
Position (kb) 1812
1813
1814 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1811.226 kb on + strand, within BWI76_RS08580 at 1811.471 kb on + strand at 1811.481 kb on + strand at 1811.667 kb on - strand, within BWI76_RS08585 at 1811.805 kb on - strand, within BWI76_RS08585 at 1811.846 kb on + strand, within BWI76_RS08585 at 1812.441 kb on - strand, within BWI76_RS08590 at 1812.443 kb on - strand, within BWI76_RS08590 at 1812.462 kb on - strand, within BWI76_RS08590 at 1812.488 kb on - strand, within BWI76_RS08590 at 1812.512 kb on + strand, within BWI76_RS08590 at 1812.678 kb on - strand, within BWI76_RS08590 at 1812.988 kb on - strand at 1813.082 kb on + strand at 1813.168 kb on - strand at 1813.170 kb on + strand at 1813.178 kb on - strand at 1813.208 kb on - strand at 1813.326 kb on - strand, within BWI76_RS08595 at 1813.337 kb on + strand, within BWI76_RS08595 at 1813.395 kb on - strand, within BWI76_RS08595 at 1813.422 kb on + strand, within BWI76_RS08595 at 1813.454 kb on + strand, within BWI76_RS08595 at 1813.518 kb on + strand, within BWI76_RS08595 at 1813.524 kb on + strand, within BWI76_RS08595 at 1813.634 kb on - strand at 1813.641 kb on + strand at 1813.641 kb on + strand at 1813.721 kb on - strand at 1814.066 kb on - strand, within BWI76_RS08600
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 1,811,226 + BWI76_RS08580 0.85 -3.4 1,811,471 + -0.3 1,811,481 + +0.6 1,811,667 - BWI76_RS08585 0.35 +2.6 1,811,805 - BWI76_RS08585 0.54 +0.6 1,811,846 + BWI76_RS08585 0.60 -1.0 1,812,441 - BWI76_RS08590 0.33 -2.3 1,812,443 - BWI76_RS08590 0.33 +0.7 1,812,462 - BWI76_RS08590 0.35 +0.5 1,812,488 - BWI76_RS08590 0.38 +0.7 1,812,512 + BWI76_RS08590 0.40 +1.3 1,812,678 - BWI76_RS08590 0.58 +1.0 1,812,988 - -0.8 1,813,082 + -1.5 1,813,168 - +0.7 1,813,170 + +1.9 1,813,178 - -0.1 1,813,208 - -0.5 1,813,326 - BWI76_RS08595 0.35 -0.0 1,813,337 + BWI76_RS08595 0.38 +0.5 1,813,395 - BWI76_RS08595 0.54 +0.5 1,813,422 + BWI76_RS08595 0.61 +0.7 1,813,454 + BWI76_RS08595 0.70 -1.2 1,813,518 + BWI76_RS08595 0.87 +0.0 1,813,524 + BWI76_RS08595 0.88 -0.7 1,813,634 - -0.1 1,813,641 + +0.3 1,813,641 + -0.5 1,813,721 - -0.1 1,814,066 - BWI76_RS08600 0.17 +1.3
Or see this region's nucleotide sequence