Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08225 and BWI76_RS08230 are separated by 99 nucleotides BWI76_RS08230 and BWI76_RS08235 are separated by 92 nucleotides BWI76_RS08235 and BWI76_RS08240 are separated by 46 nucleotides BWI76_RS08240 and BWI76_RS08245 are separated by 52 nucleotides
BWI76_RS08225: BWI76_RS08225 - glutamine--tRNA ligase, at 1,741,165 to 1,742,832
_RS08225
BWI76_RS08230: BWI76_RS08230 - hypothetical protein, at 1,742,932 to 1,743,176
_RS08230
BWI76_RS08235: BWI76_RS08235 - chitoporin, at 1,743,269 to 1,744,672
_RS08235
BWI76_RS08240: BWI76_RS08240 - hypothetical protein, at 1,744,719 to 1,745,051
_RS08240
BWI76_RS08245: BWI76_RS08245 - MFS transporter, at 1,745,104 to 1,746,399
_RS08245
Position (kb)
1743
1744
1745 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1743.053 kb on + strand, within BWI76_RS08230 at 1743.061 kb on - strand, within BWI76_RS08230 at 1743.061 kb on - strand, within BWI76_RS08230 at 1743.083 kb on - strand, within BWI76_RS08230 at 1743.225 kb on + strand at 1743.250 kb on + strand at 1743.381 kb on + strand at 1743.540 kb on + strand, within BWI76_RS08235 at 1743.554 kb on - strand, within BWI76_RS08235 at 1743.597 kb on + strand, within BWI76_RS08235 at 1743.609 kb on - strand, within BWI76_RS08235 at 1743.667 kb on + strand, within BWI76_RS08235 at 1743.667 kb on + strand, within BWI76_RS08235 at 1743.675 kb on - strand, within BWI76_RS08235 at 1743.675 kb on - strand, within BWI76_RS08235 at 1743.716 kb on + strand, within BWI76_RS08235 at 1743.782 kb on - strand, within BWI76_RS08235 at 1743.886 kb on + strand, within BWI76_RS08235 at 1744.000 kb on + strand, within BWI76_RS08235 at 1744.000 kb on + strand, within BWI76_RS08235 at 1744.008 kb on - strand, within BWI76_RS08235 at 1744.008 kb on - strand, within BWI76_RS08235 at 1745.045 kb on + strand at 1745.390 kb on - strand, within BWI76_RS08245 at 1745.401 kb on - strand, within BWI76_RS08245 at 1745.440 kb on + strand, within BWI76_RS08245 at 1745.456 kb on + strand, within BWI76_RS08245 at 1745.570 kb on + strand, within BWI76_RS08245
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 1,743,053 + BWI76_RS08230 0.49 +0.4 1,743,061 - BWI76_RS08230 0.53 -1.2 1,743,061 - BWI76_RS08230 0.53 -1.8 1,743,083 - BWI76_RS08230 0.62 +1.0 1,743,225 + +0.3 1,743,250 + +2.2 1,743,381 + -1.0 1,743,540 + BWI76_RS08235 0.19 -0.7 1,743,554 - BWI76_RS08235 0.20 -1.6 1,743,597 + BWI76_RS08235 0.23 -1.8 1,743,609 - BWI76_RS08235 0.24 +0.3 1,743,667 + BWI76_RS08235 0.28 -0.8 1,743,667 + BWI76_RS08235 0.28 +0.0 1,743,675 - BWI76_RS08235 0.29 -0.6 1,743,675 - BWI76_RS08235 0.29 -1.7 1,743,716 + BWI76_RS08235 0.32 -1.0 1,743,782 - BWI76_RS08235 0.37 -2.2 1,743,886 + BWI76_RS08235 0.44 -1.3 1,744,000 + BWI76_RS08235 0.52 +1.6 1,744,000 + BWI76_RS08235 0.52 +0.0 1,744,008 - BWI76_RS08235 0.53 -0.3 1,744,008 - BWI76_RS08235 0.53 +1.6 1,745,045 + +0.6 1,745,390 - BWI76_RS08245 0.22 -3.6 1,745,401 - BWI76_RS08245 0.23 -0.8 1,745,440 + BWI76_RS08245 0.26 -1.6 1,745,456 + BWI76_RS08245 0.27 -0.9 1,745,570 + BWI76_RS08245 0.36 -0.0
Or see this region's nucleotide sequence