Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07615 and BWI76_RS07620 are separated by 10 nucleotides BWI76_RS07620 and BWI76_RS07625 overlap by 84 nucleotides BWI76_RS07625 and BWI76_RS07630 overlap by 4 nucleotides
BWI76_RS07615: BWI76_RS07615 - betaine-aldehyde dehydrogenase, at 1,613,138 to 1,614,610
_RS07615
BWI76_RS07620: BWI76_RS07620 - transcriptional regulator BetI, at 1,614,621 to 1,615,214
_RS07620
BWI76_RS07625: BWI76_RS07625 - hypothetical protein, at 1,615,131 to 1,615,346
_RS07625
BWI76_RS07630: BWI76_RS07630 - high-affinity choline transporter BetT, at 1,615,343 to 1,617,376
_RS07630
Position (kb)
1615
1616 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1614.458 kb on + strand, within BWI76_RS07615 at 1614.501 kb on + strand at 1614.501 kb on + strand at 1614.605 kb on + strand at 1614.702 kb on + strand, within BWI76_RS07620 at 1614.818 kb on + strand, within BWI76_RS07620 at 1615.001 kb on - strand, within BWI76_RS07620 at 1615.039 kb on + strand, within BWI76_RS07620 at 1615.467 kb on + strand at 1615.478 kb on + strand at 1615.552 kb on + strand, within BWI76_RS07630 at 1615.590 kb on + strand at 1615.730 kb on - strand, within BWI76_RS07630 at 1616.014 kb on + strand at 1616.151 kb on + strand, within BWI76_RS07630 at 1616.170 kb on + strand, within BWI76_RS07630 at 1616.186 kb on - strand, within BWI76_RS07630
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 1,614,458 + BWI76_RS07615 0.90 -2.1 1,614,501 + -1.3 1,614,501 + +0.4 1,614,605 + +0.0 1,614,702 + BWI76_RS07620 0.14 +0.3 1,614,818 + BWI76_RS07620 0.33 -3.0 1,615,001 - BWI76_RS07620 0.64 +1.1 1,615,039 + BWI76_RS07620 0.70 +1.3 1,615,467 + -0.2 1,615,478 + -2.1 1,615,552 + BWI76_RS07630 0.10 -0.6 1,615,590 + +1.0 1,615,730 - BWI76_RS07630 0.19 -0.3 1,616,014 + -1.3 1,616,151 + BWI76_RS07630 0.40 +0.7 1,616,170 + BWI76_RS07630 0.41 +1.0 1,616,186 - BWI76_RS07630 0.41 +0.2
Or see this region's nucleotide sequence