Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04590 and BWI76_RS04595 are separated by 337 nucleotides BWI76_RS04595 and BWI76_RS04600 are separated by 128 nucleotides BWI76_RS04600 and BWI76_RS04605 overlap by 14 nucleotides
BWI76_RS04590: BWI76_RS04590 - ribulokinase, at 954,731 to 956,440
_RS04590
BWI76_RS04595: BWI76_RS04595 - DNA-binding transcriptional regulator AraC, at 956,778 to 957,623
_RS04595
BWI76_RS04600: BWI76_RS04600 - inner membrane protein, at 957,752 to 958,519
_RS04600
BWI76_RS04605: BWI76_RS04605 - thiamine ABC transporter ATP-binding protein, at 958,506 to 959,207
_RS04605
Position (kb) 956
957
958 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 955.911 kb on - strand, within BWI76_RS04590 at 956.018 kb on - strand, within BWI76_RS04590 at 956.029 kb on + strand, within BWI76_RS04590 at 956.139 kb on + strand, within BWI76_RS04590 at 956.223 kb on - strand, within BWI76_RS04590 at 956.310 kb on + strand at 956.352 kb on - strand at 956.484 kb on + strand at 956.487 kb on - strand at 956.539 kb on - strand at 956.749 kb on + strand at 956.908 kb on + strand, within BWI76_RS04595 at 956.916 kb on - strand, within BWI76_RS04595 at 956.921 kb on - strand, within BWI76_RS04595 at 956.966 kb on + strand, within BWI76_RS04595 at 957.063 kb on + strand, within BWI76_RS04595 at 957.197 kb on - strand, within BWI76_RS04595 at 957.217 kb on + strand, within BWI76_RS04595 at 957.225 kb on - strand, within BWI76_RS04595 at 957.302 kb on + strand, within BWI76_RS04595 at 957.487 kb on - strand, within BWI76_RS04595 at 957.491 kb on + strand, within BWI76_RS04595 at 957.710 kb on - strand at 957.711 kb on + strand at 957.882 kb on + strand, within BWI76_RS04600 at 957.910 kb on + strand, within BWI76_RS04600 at 958.400 kb on + strand, within BWI76_RS04600
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 955,911 - BWI76_RS04590 0.69 +0.3 956,018 - BWI76_RS04590 0.75 -0.9 956,029 + BWI76_RS04590 0.76 +0.5 956,139 + BWI76_RS04590 0.82 +0.1 956,223 - BWI76_RS04590 0.87 +0.6 956,310 + -1.7 956,352 - -2.7 956,484 + +0.0 956,487 - +1.7 956,539 - -1.7 956,749 + -0.5 956,908 + BWI76_RS04595 0.15 +0.4 956,916 - BWI76_RS04595 0.16 -0.1 956,921 - BWI76_RS04595 0.17 +0.9 956,966 + BWI76_RS04595 0.22 +0.5 957,063 + BWI76_RS04595 0.34 +1.3 957,197 - BWI76_RS04595 0.50 +1.4 957,217 + BWI76_RS04595 0.52 +2.6 957,225 - BWI76_RS04595 0.53 +0.4 957,302 + BWI76_RS04595 0.62 +0.1 957,487 - BWI76_RS04595 0.84 +3.3 957,491 + BWI76_RS04595 0.84 +1.0 957,710 - +0.0 957,711 + -2.3 957,882 + BWI76_RS04600 0.17 +0.4 957,910 + BWI76_RS04600 0.21 +0.7 958,400 + BWI76_RS04600 0.84 +0.1
Or see this region's nucleotide sequence