Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04100 and BWI76_RS04105 are separated by 217 nucleotides BWI76_RS04105 and BWI76_RS04110 are separated by 123 nucleotides BWI76_RS04110 and BWI76_RS04115 are separated by 120 nucleotides
BWI76_RS04100: BWI76_RS04100 - peptide chain release factor 3, at 843,057 to 844,646
_RS04100
BWI76_RS04105: BWI76_RS04105 - molecular chaperone OsmY, at 844,864 to 845,475
_RS04105
BWI76_RS04110: BWI76_RS04110 - DUF1328 domain-containing protein, at 845,599 to 845,760
_RS04110
BWI76_RS04115: BWI76_RS04115 - patatin family protein, at 845,881 to 846,954
_RS04115
Position (kb) 845
846 Strain fitness (log2 ratio) -2
-1
0
1
2 at 844.899 kb on - strand at 844.923 kb on - strand at 844.951 kb on + strand, within BWI76_RS04105 at 844.951 kb on + strand, within BWI76_RS04105 at 844.951 kb on + strand, within BWI76_RS04105 at 844.959 kb on - strand, within BWI76_RS04105 at 845.063 kb on - strand, within BWI76_RS04105 at 845.063 kb on - strand, within BWI76_RS04105 at 845.097 kb on + strand, within BWI76_RS04105 at 845.133 kb on - strand, within BWI76_RS04105 at 845.141 kb on + strand, within BWI76_RS04105 at 845.259 kb on + strand, within BWI76_RS04105 at 845.368 kb on - strand, within BWI76_RS04105 at 845.385 kb on - strand, within BWI76_RS04105 at 845.409 kb on + strand, within BWI76_RS04105 at 845.436 kb on - strand at 845.527 kb on + strand at 845.527 kb on + strand at 845.527 kb on + strand at 845.527 kb on - strand at 845.535 kb on - strand at 845.535 kb on - strand at 845.540 kb on - strand at 845.582 kb on + strand at 845.589 kb on + strand at 845.590 kb on - strand at 845.647 kb on + strand, within BWI76_RS04110 at 845.655 kb on - strand, within BWI76_RS04110 at 845.657 kb on + strand, within BWI76_RS04110 at 845.667 kb on + strand, within BWI76_RS04110 at 845.687 kb on - strand, within BWI76_RS04110 at 845.711 kb on + strand, within BWI76_RS04110 at 845.763 kb on - strand at 845.907 kb on + strand at 845.912 kb on + strand at 845.951 kb on + strand at 845.959 kb on - strand at 845.959 kb on - strand at 845.964 kb on - strand at 845.971 kb on + strand at 845.980 kb on - strand at 846.420 kb on - strand, within BWI76_RS04115 at 846.514 kb on + strand, within BWI76_RS04115 at 846.519 kb on + strand, within BWI76_RS04115 at 846.521 kb on + strand, within BWI76_RS04115 at 846.527 kb on - strand, within BWI76_RS04115 at 846.633 kb on - strand, within BWI76_RS04115 at 846.711 kb on - strand, within BWI76_RS04115 at 846.711 kb on - strand, within BWI76_RS04115 at 846.746 kb on - strand, within BWI76_RS04115
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 844,899 - +2.4 844,923 - +1.0 844,951 + BWI76_RS04105 0.14 +0.8 844,951 + BWI76_RS04105 0.14 -0.0 844,951 + BWI76_RS04105 0.14 -0.5 844,959 - BWI76_RS04105 0.16 -0.4 845,063 - BWI76_RS04105 0.33 -0.2 845,063 - BWI76_RS04105 0.33 -1.1 845,097 + BWI76_RS04105 0.38 +1.1 845,133 - BWI76_RS04105 0.44 +0.0 845,141 + BWI76_RS04105 0.45 +1.1 845,259 + BWI76_RS04105 0.65 +1.0 845,368 - BWI76_RS04105 0.82 -0.6 845,385 - BWI76_RS04105 0.85 -0.5 845,409 + BWI76_RS04105 0.89 -0.5 845,436 - +1.6 845,527 + -0.5 845,527 + -1.0 845,527 + -0.5 845,527 - -1.0 845,535 - -1.8 845,535 - -0.5 845,540 - +0.7 845,582 + -0.5 845,589 + -0.6 845,590 - -0.2 845,647 + BWI76_RS04110 0.30 +0.4 845,655 - BWI76_RS04110 0.35 -0.1 845,657 + BWI76_RS04110 0.36 +1.4 845,667 + BWI76_RS04110 0.42 +1.4 845,687 - BWI76_RS04110 0.54 +1.0 845,711 + BWI76_RS04110 0.69 +1.0 845,763 - +0.0 845,907 + -0.1 845,912 + +0.1 845,951 + -0.0 845,959 - +0.1 845,959 - +0.1 845,964 - +0.1 845,971 + -0.5 845,980 - -0.6 846,420 - BWI76_RS04115 0.50 -1.5 846,514 + BWI76_RS04115 0.59 +0.7 846,519 + BWI76_RS04115 0.59 +1.5 846,521 + BWI76_RS04115 0.60 +0.5 846,527 - BWI76_RS04115 0.60 -1.0 846,633 - BWI76_RS04115 0.70 -0.6 846,711 - BWI76_RS04115 0.77 +0.5 846,711 - BWI76_RS04115 0.77 -2.3 846,746 - BWI76_RS04115 0.81 -1.0
Or see this region's nucleotide sequence