Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03085 and BWI76_RS03090 are separated by 526 nucleotides BWI76_RS03090 and BWI76_RS03095 are separated by 48 nucleotides
BWI76_RS03085: BWI76_RS03085 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) <small>(from data)</small>, at 632,739 to 634,652
_RS03085
BWI76_RS03090: BWI76_RS03090 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) <small>(from data)</small>, at 635,179 to 637,119
_RS03090
BWI76_RS03095: BWI76_RS03095 - Inositol 2-dehydrogenase (EC 1.1.1.18) <small>(from data)</small>, at 637,168 to 638,181
_RS03095
Position (kb) 635
636
637
638 Strain fitness (log2 ratio) -2
-1
0
1
2 at 634.214 kb on - strand, within BWI76_RS03085 at 634.301 kb on - strand, within BWI76_RS03085 at 634.422 kb on + strand, within BWI76_RS03085 at 634.430 kb on - strand, within BWI76_RS03085 at 634.430 kb on - strand, within BWI76_RS03085 at 634.562 kb on + strand at 634.573 kb on + strand at 634.979 kb on - strand at 634.997 kb on + strand at 634.997 kb on + strand at 635.118 kb on + strand at 635.226 kb on + strand at 635.325 kb on - strand at 635.661 kb on + strand, within BWI76_RS03090 at 635.684 kb on - strand, within BWI76_RS03090 at 635.746 kb on + strand, within BWI76_RS03090 at 635.840 kb on - strand, within BWI76_RS03090 at 635.940 kb on + strand at 635.974 kb on + strand, within BWI76_RS03090 at 635.982 kb on - strand, within BWI76_RS03090 at 636.049 kb on - strand, within BWI76_RS03090 at 636.326 kb on + strand, within BWI76_RS03090 at 636.425 kb on + strand, within BWI76_RS03090 at 636.610 kb on - strand, within BWI76_RS03090 at 636.627 kb on - strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.703 kb on + strand, within BWI76_RS03090 at 636.709 kb on - strand, within BWI76_RS03090 at 636.709 kb on - strand, within BWI76_RS03090 at 636.709 kb on - strand, within BWI76_RS03090 at 636.848 kb on + strand, within BWI76_RS03090 at 636.985 kb on - strand at 637.410 kb on - strand, within BWI76_RS03095 at 637.439 kb on + strand, within BWI76_RS03095 at 637.923 kb on + strand, within BWI76_RS03095
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 634,214 - BWI76_RS03085 0.77 +1.1 634,301 - BWI76_RS03085 0.82 -0.1 634,422 + BWI76_RS03085 0.88 -0.5 634,430 - BWI76_RS03085 0.88 +0.1 634,430 - BWI76_RS03085 0.88 -0.3 634,562 + -2.4 634,573 + -1.3 634,979 - +0.4 634,997 + -0.7 634,997 + +1.2 635,118 + +0.8 635,226 + +0.8 635,325 - -1.2 635,661 + BWI76_RS03090 0.25 -0.5 635,684 - BWI76_RS03090 0.26 +1.1 635,746 + BWI76_RS03090 0.29 +0.5 635,840 - BWI76_RS03090 0.34 +0.4 635,940 + +0.1 635,974 + BWI76_RS03090 0.41 +0.8 635,982 - BWI76_RS03090 0.41 -0.5 636,049 - BWI76_RS03090 0.45 +1.1 636,326 + BWI76_RS03090 0.59 +0.5 636,425 + BWI76_RS03090 0.64 -0.1 636,610 - BWI76_RS03090 0.74 -0.0 636,627 - BWI76_RS03090 0.75 -1.6 636,701 + BWI76_RS03090 0.78 +1.2 636,701 + BWI76_RS03090 0.78 -0.0 636,701 + BWI76_RS03090 0.78 -0.3 636,701 + BWI76_RS03090 0.78 +2.2 636,701 + BWI76_RS03090 0.78 -0.1 636,703 + BWI76_RS03090 0.79 -0.8 636,709 - BWI76_RS03090 0.79 -1.0 636,709 - BWI76_RS03090 0.79 -0.3 636,709 - BWI76_RS03090 0.79 -0.4 636,848 + BWI76_RS03090 0.86 -0.4 636,985 - +0.1 637,410 - BWI76_RS03095 0.24 +0.1 637,439 + BWI76_RS03095 0.27 -0.6 637,923 + BWI76_RS03095 0.74 -0.8
Or see this region's nucleotide sequence