Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02305 and BWI76_RS02310 are separated by 67 nucleotides BWI76_RS02310 and BWI76_RS02315 are separated by 35 nucleotides
BWI76_RS02305: BWI76_RS02305 - hypothetical protein, at 473,077 to 475,164
_RS02305
BWI76_RS02310: BWI76_RS02310 - transcriptional regulator, at 475,232 to 475,807
_RS02310
BWI76_RS02315: BWI76_RS02315 - protein-disulfide reductase DsbD, at 475,843 to 477,600
_RS02315
Position (kb) 475
476 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 474.351 kb on + strand, within BWI76_RS02305 at 474.390 kb on + strand, within BWI76_RS02305 at 474.390 kb on + strand, within BWI76_RS02305 at 474.395 kb on - strand, within BWI76_RS02305 at 474.400 kb on - strand, within BWI76_RS02305 at 474.523 kb on + strand, within BWI76_RS02305 at 474.664 kb on + strand, within BWI76_RS02305 at 474.672 kb on - strand, within BWI76_RS02305 at 474.890 kb on + strand, within BWI76_RS02305 at 474.996 kb on - strand at 475.014 kb on + strand at 475.121 kb on + strand at 475.127 kb on - strand at 475.146 kb on + strand at 475.172 kb on - strand at 475.172 kb on - strand at 475.251 kb on + strand at 475.251 kb on + strand at 475.328 kb on - strand, within BWI76_RS02310 at 475.346 kb on + strand, within BWI76_RS02310 at 475.540 kb on + strand, within BWI76_RS02310 at 475.548 kb on - strand, within BWI76_RS02310 at 475.592 kb on - strand, within BWI76_RS02310 at 475.688 kb on - strand, within BWI76_RS02310 at 475.699 kb on + strand, within BWI76_RS02310 at 475.854 kb on - strand at 475.906 kb on - strand at 475.973 kb on - strand at 476.037 kb on + strand, within BWI76_RS02315 at 476.045 kb on - strand, within BWI76_RS02315 at 476.078 kb on - strand, within BWI76_RS02315 at 476.263 kb on - strand, within BWI76_RS02315 at 476.270 kb on + strand, within BWI76_RS02315 at 476.492 kb on + strand, within BWI76_RS02315 at 476.586 kb on + strand, within BWI76_RS02315 at 476.686 kb on + strand, within BWI76_RS02315 at 476.694 kb on - strand, within BWI76_RS02315 at 476.786 kb on - strand, within BWI76_RS02315
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 474,351 + BWI76_RS02305 0.61 -2.2 474,390 + BWI76_RS02305 0.63 -1.0 474,390 + BWI76_RS02305 0.63 -1.0 474,395 - BWI76_RS02305 0.63 +1.0 474,400 - BWI76_RS02305 0.63 -1.2 474,523 + BWI76_RS02305 0.69 -1.1 474,664 + BWI76_RS02305 0.76 -1.1 474,672 - BWI76_RS02305 0.76 +0.5 474,890 + BWI76_RS02305 0.87 -0.1 474,996 - -1.4 475,014 + -1.6 475,121 + +1.0 475,127 - +0.6 475,146 + +2.9 475,172 - +0.1 475,172 - -0.7 475,251 + +1.7 475,251 + -0.1 475,328 - BWI76_RS02310 0.17 +1.0 475,346 + BWI76_RS02310 0.20 -1.6 475,540 + BWI76_RS02310 0.53 -1.3 475,548 - BWI76_RS02310 0.55 +0.3 475,592 - BWI76_RS02310 0.62 -0.8 475,688 - BWI76_RS02310 0.79 -1.1 475,699 + BWI76_RS02310 0.81 +0.7 475,854 - +0.8 475,906 - +0.1 475,973 - -0.8 476,037 + BWI76_RS02315 0.11 -2.6 476,045 - BWI76_RS02315 0.11 -0.5 476,078 - BWI76_RS02315 0.13 -1.8 476,263 - BWI76_RS02315 0.24 +0.6 476,270 + BWI76_RS02315 0.24 +1.1 476,492 + BWI76_RS02315 0.37 -0.2 476,586 + BWI76_RS02315 0.42 -1.9 476,686 + BWI76_RS02315 0.48 -0.7 476,694 - BWI76_RS02315 0.48 -1.6 476,786 - BWI76_RS02315 0.54 -0.2
Or see this region's nucleotide sequence