Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02145 and BWI76_RS02150 overlap by 17 nucleotides BWI76_RS02150 and BWI76_RS02155 are separated by 10 nucleotides BWI76_RS02155 and BWI76_RS02160 overlap by 22 nucleotides
BWI76_RS02145: BWI76_RS02145 - allose kinase, at 446,104 to 447,030
_RS02145
BWI76_RS02150: BWI76_RS02150 - allulose-6-phosphate 3-epimerase, at 447,014 to 447,709
_RS02150
BWI76_RS02155: BWI76_RS02155 - D-allose transporter subunit, at 447,720 to 448,700
_RS02155
BWI76_RS02160: BWI76_RS02160 - D-allose transporter ATP-binding protein, at 448,679 to 450,211
_RS02160
Position (kb) 447
448 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 446.026 kb on + strand at 446.026 kb on + strand at 446.027 kb on + strand at 446.056 kb on + strand at 446.058 kb on - strand at 446.062 kb on + strand at 446.077 kb on - strand at 446.077 kb on - strand at 446.082 kb on + strand at 446.088 kb on + strand at 446.091 kb on - strand at 446.095 kb on + strand at 446.101 kb on + strand at 446.102 kb on - strand at 446.103 kb on - strand at 446.109 kb on - strand at 446.111 kb on + strand at 446.117 kb on + strand at 446.117 kb on - strand at 446.117 kb on - strand at 446.127 kb on + strand at 446.129 kb on - strand at 446.135 kb on - strand at 446.135 kb on - strand at 446.148 kb on + strand at 446.155 kb on + strand at 446.158 kb on - strand at 446.158 kb on - strand at 446.158 kb on - strand at 446.167 kb on + strand at 446.167 kb on + strand at 446.167 kb on + strand at 446.167 kb on + strand at 446.167 kb on + strand at 446.167 kb on + strand at 446.173 kb on - strand at 446.173 kb on - strand at 446.175 kb on - strand at 446.175 kb on - strand at 446.175 kb on - strand at 446.197 kb on + strand, within BWI76_RS02145 at 446.197 kb on - strand, within BWI76_RS02145 at 446.197 kb on - strand, within BWI76_RS02145 at 446.212 kb on - strand, within BWI76_RS02145 at 446.222 kb on - strand, within BWI76_RS02145 at 446.229 kb on + strand, within BWI76_RS02145 at 446.234 kb on + strand, within BWI76_RS02145 at 446.234 kb on + strand, within BWI76_RS02145 at 446.237 kb on - strand, within BWI76_RS02145 at 446.241 kb on - strand, within BWI76_RS02145 at 446.242 kb on - strand, within BWI76_RS02145 at 446.262 kb on - strand, within BWI76_RS02145 at 446.286 kb on - strand, within BWI76_RS02145 at 446.288 kb on - strand, within BWI76_RS02145 at 446.306 kb on - strand, within BWI76_RS02145 at 446.322 kb on + strand, within BWI76_RS02145 at 446.322 kb on + strand, within BWI76_RS02145 at 446.340 kb on - strand, within BWI76_RS02145 at 446.425 kb on + strand, within BWI76_RS02145 at 446.446 kb on + strand, within BWI76_RS02145 at 446.452 kb on - strand, within BWI76_RS02145 at 446.506 kb on - strand, within BWI76_RS02145 at 446.596 kb on + strand, within BWI76_RS02145 at 446.604 kb on - strand, within BWI76_RS02145 at 446.604 kb on - strand, within BWI76_RS02145 at 446.628 kb on + strand, within BWI76_RS02145 at 446.645 kb on - strand, within BWI76_RS02145 at 446.707 kb on + strand, within BWI76_RS02145 at 446.715 kb on - strand, within BWI76_RS02145 at 446.715 kb on - strand, within BWI76_RS02145 at 446.717 kb on + strand, within BWI76_RS02145 at 446.717 kb on + strand, within BWI76_RS02145 at 446.717 kb on + strand, within BWI76_RS02145 at 446.725 kb on - strand, within BWI76_RS02145 at 446.725 kb on - strand, within BWI76_RS02145 at 446.725 kb on - strand, within BWI76_RS02145 at 446.730 kb on + strand, within BWI76_RS02145 at 446.913 kb on + strand, within BWI76_RS02145 at 446.916 kb on - strand, within BWI76_RS02145 at 446.943 kb on + strand at 446.985 kb on - strand at 446.985 kb on - strand at 446.998 kb on - strand at 447.029 kb on - strand at 447.123 kb on - strand, within BWI76_RS02150 at 447.481 kb on - strand, within BWI76_RS02150 at 447.487 kb on + strand, within BWI76_RS02150 at 447.492 kb on + strand, within BWI76_RS02150 at 447.500 kb on - strand, within BWI76_RS02150 at 447.500 kb on - strand, within BWI76_RS02150 at 447.581 kb on + strand, within BWI76_RS02150 at 447.676 kb on + strand at 447.676 kb on + strand at 447.676 kb on + strand at 447.684 kb on - strand at 447.686 kb on - strand at 447.686 kb on - strand at 447.694 kb on - strand at 447.694 kb on - strand at 447.792 kb on + strand at 448.074 kb on - strand, within BWI76_RS02155 at 448.221 kb on + strand, within BWI76_RS02155 at 448.229 kb on - strand, within BWI76_RS02155 at 448.412 kb on + strand, within BWI76_RS02155 at 448.412 kb on + strand, within BWI76_RS02155 at 448.564 kb on - strand, within BWI76_RS02155 at 448.638 kb on - strand at 448.638 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 446,026 + -0.1 446,026 + -2.5 446,027 + -0.0 446,056 + -2.4 446,058 - -0.8 446,062 + +0.5 446,077 - -1.2 446,077 - +0.3 446,082 + +2.2 446,088 + +1.7 446,091 - -0.2 446,095 + +0.2 446,101 + +1.8 446,102 - -0.6 446,103 - +0.3 446,109 - +1.0 446,111 + +0.2 446,117 + -1.5 446,117 - -0.0 446,117 - -0.4 446,127 + -0.1 446,129 - -0.2 446,135 - +0.1 446,135 - +0.5 446,148 + -0.7 446,155 + -1.2 446,158 - -0.0 446,158 - -1.4 446,158 - -0.0 446,167 + +1.0 446,167 + +0.7 446,167 + +2.8 446,167 + -1.1 446,167 + -2.1 446,167 + +1.0 446,173 - +1.0 446,173 - +1.0 446,175 - +0.3 446,175 - -0.5 446,175 - -0.3 446,197 + BWI76_RS02145 0.10 -0.2 446,197 - BWI76_RS02145 0.10 -1.6 446,197 - BWI76_RS02145 0.10 -0.6 446,212 - BWI76_RS02145 0.12 -0.6 446,222 - BWI76_RS02145 0.13 +1.1 446,229 + BWI76_RS02145 0.13 -1.8 446,234 + BWI76_RS02145 0.14 +1.0 446,234 + BWI76_RS02145 0.14 +0.3 446,237 - BWI76_RS02145 0.14 -1.9 446,241 - BWI76_RS02145 0.15 -0.9 446,242 - BWI76_RS02145 0.15 +0.1 446,262 - BWI76_RS02145 0.17 +1.2 446,286 - BWI76_RS02145 0.20 +0.3 446,288 - BWI76_RS02145 0.20 +2.2 446,306 - BWI76_RS02145 0.22 -0.6 446,322 + BWI76_RS02145 0.24 -2.6 446,322 + BWI76_RS02145 0.24 -2.4 446,340 - BWI76_RS02145 0.25 +2.0 446,425 + BWI76_RS02145 0.35 +0.3 446,446 + BWI76_RS02145 0.37 +0.5 446,452 - BWI76_RS02145 0.38 +0.5 446,506 - BWI76_RS02145 0.43 +1.0 446,596 + BWI76_RS02145 0.53 +0.7 446,604 - BWI76_RS02145 0.54 +1.0 446,604 - BWI76_RS02145 0.54 -0.0 446,628 + BWI76_RS02145 0.57 +0.8 446,645 - BWI76_RS02145 0.58 -0.6 446,707 + BWI76_RS02145 0.65 +1.4 446,715 - BWI76_RS02145 0.66 +0.9 446,715 - BWI76_RS02145 0.66 -0.3 446,717 + BWI76_RS02145 0.66 +1.0 446,717 + BWI76_RS02145 0.66 +0.6 446,717 + BWI76_RS02145 0.66 +0.8 446,725 - BWI76_RS02145 0.67 +0.6 446,725 - BWI76_RS02145 0.67 -1.0 446,725 - BWI76_RS02145 0.67 -3.5 446,730 + BWI76_RS02145 0.68 +0.7 446,913 + BWI76_RS02145 0.87 -1.0 446,916 - BWI76_RS02145 0.88 +0.3 446,943 + -0.2 446,985 - -1.8 446,985 - -0.8 446,998 - +1.6 447,029 - +1.8 447,123 - BWI76_RS02150 0.16 +0.7 447,481 - BWI76_RS02150 0.67 +1.0 447,487 + BWI76_RS02150 0.68 -2.6 447,492 + BWI76_RS02150 0.69 +0.8 447,500 - BWI76_RS02150 0.70 +2.4 447,500 - BWI76_RS02150 0.70 -2.3 447,581 + BWI76_RS02150 0.81 -0.5 447,676 + -1.0 447,676 + +0.8 447,676 + -1.5 447,684 - +1.0 447,686 - -0.8 447,686 - +0.1 447,694 - +0.7 447,694 - -0.5 447,792 + +0.9 448,074 - BWI76_RS02155 0.36 +0.3 448,221 + BWI76_RS02155 0.51 -0.4 448,229 - BWI76_RS02155 0.52 -1.6 448,412 + BWI76_RS02155 0.71 -1.4 448,412 + BWI76_RS02155 0.71 +0.3 448,564 - BWI76_RS02155 0.86 -1.1 448,638 - -0.1 448,638 - -0.1
Or see this region's nucleotide sequence