Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01685 and BWI76_RS01690 are separated by 185 nucleotides BWI76_RS01690 and BWI76_RS01695 are separated by 261 nucleotides
BWI76_RS01685: BWI76_RS01685 - methionine synthase, at 343,151 to 346,834
_RS01685
BWI76_RS01690: BWI76_RS01690 - membrane protein, at 347,020 to 348,651
_RS01690
BWI76_RS01695: BWI76_RS01695 - IS110 family transposase, at 348,913 to 349,893
_RS01695
Position (kb) 347
348
349 Strain fitness (log2 ratio) -1
0
1
2 at 346.086 kb on - strand, within BWI76_RS01685 at 346.116 kb on - strand, within BWI76_RS01685 at 346.329 kb on + strand, within BWI76_RS01685 at 346.413 kb on - strand, within BWI76_RS01685 at 346.575 kb on + strand at 346.610 kb on - strand at 346.756 kb on - strand at 346.872 kb on + strand at 346.909 kb on + strand at 346.909 kb on + strand at 346.910 kb on - strand at 346.983 kb on - strand at 347.029 kb on + strand at 347.043 kb on - strand at 347.097 kb on - strand at 347.097 kb on - strand at 347.121 kb on - strand at 347.263 kb on + strand, within BWI76_RS01690 at 347.321 kb on + strand, within BWI76_RS01690 at 347.478 kb on - strand, within BWI76_RS01690 at 347.495 kb on - strand, within BWI76_RS01690 at 347.657 kb on - strand, within BWI76_RS01690 at 347.813 kb on - strand, within BWI76_RS01690 at 347.927 kb on - strand, within BWI76_RS01690 at 347.994 kb on + strand, within BWI76_RS01690 at 347.994 kb on + strand, within BWI76_RS01690 at 348.590 kb on + strand at 348.598 kb on - strand at 348.607 kb on - strand at 348.669 kb on + strand at 348.971 kb on + strand at 349.010 kb on + strand at 349.027 kb on + strand, within BWI76_RS01695 at 349.073 kb on + strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.093 kb on + strand, within BWI76_RS01695 at 349.097 kb on + strand, within BWI76_RS01695 at 349.102 kb on + strand, within BWI76_RS01695 at 349.492 kb on - strand, within BWI76_RS01695 at 349.569 kb on + strand, within BWI76_RS01695 at 349.607 kb on + strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 346,086 - BWI76_RS01685 0.80 +1.0 346,116 - BWI76_RS01685 0.80 +0.7 346,329 + BWI76_RS01685 0.86 -0.4 346,413 - BWI76_RS01685 0.89 +0.3 346,575 + -1.4 346,610 - +1.0 346,756 - -0.2 346,872 + -1.7 346,909 + -1.2 346,909 + -0.2 346,910 - +0.9 346,983 - +0.5 347,029 + -0.9 347,043 - +1.1 347,097 - +1.2 347,097 - +0.4 347,121 - +1.1 347,263 + BWI76_RS01690 0.15 -0.0 347,321 + BWI76_RS01690 0.18 +0.2 347,478 - BWI76_RS01690 0.28 +1.1 347,495 - BWI76_RS01690 0.29 +0.2 347,657 - BWI76_RS01690 0.39 +0.4 347,813 - BWI76_RS01690 0.49 -1.5 347,927 - BWI76_RS01690 0.56 -0.4 347,994 + BWI76_RS01690 0.60 +2.0 347,994 + BWI76_RS01690 0.60 +1.5 348,590 + -0.7 348,598 - -1.4 348,607 - +1.1 348,669 + -1.5 348,971 + +0.9 349,010 + +0.1 349,027 + BWI76_RS01695 0.12 +0.6 349,073 + BWI76_RS01695 0.16 -0.3 349,081 - BWI76_RS01695 0.17 +1.0 349,081 - BWI76_RS01695 0.17 +1.5 349,081 - BWI76_RS01695 0.17 +0.1 349,081 - BWI76_RS01695 0.17 +1.9 349,093 + BWI76_RS01695 0.18 +1.2 349,097 + BWI76_RS01695 0.19 +0.4 349,102 + BWI76_RS01695 0.19 -0.7 349,492 - BWI76_RS01695 0.59 -0.9 349,569 + BWI76_RS01695 0.67 -0.2 349,607 + BWI76_RS01695 0.71 +0.2 349,633 - BWI76_RS01695 0.73 +1.2 349,633 - BWI76_RS01695 0.73 +1.4
Or see this region's nucleotide sequence