Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01655 and BWI76_RS01660 are separated by 259 nucleotides BWI76_RS01660 and BWI76_RS01665 are separated by 31 nucleotides
BWI76_RS01655: BWI76_RS01655 - homoserine O-succinyltransferase, at 335,729 to 336,658
_RS01655
BWI76_RS01660: BWI76_RS01660 - malate synthase A, at 336,918 to 338,519
_RS01660
BWI76_RS01665: BWI76_RS01665 - Isocitrate lyase (EC 4.1.3.1) <small>(from data)</small>, at 338,551 to 339,855
_RS01665
Position (kb) 336
337
338
339 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 335.918 kb on - strand, within BWI76_RS01655 at 336.033 kb on + strand, within BWI76_RS01655 at 336.115 kb on - strand, within BWI76_RS01655 at 336.115 kb on - strand, within BWI76_RS01655 at 336.135 kb on + strand, within BWI76_RS01655 at 336.143 kb on - strand, within BWI76_RS01655 at 336.447 kb on + strand, within BWI76_RS01655 at 336.447 kb on + strand, within BWI76_RS01655 at 336.455 kb on - strand, within BWI76_RS01655 at 336.621 kb on - strand at 336.660 kb on + strand at 336.771 kb on + strand at 336.909 kb on - strand at 336.970 kb on - strand at 337.020 kb on + strand at 337.284 kb on - strand, within BWI76_RS01660 at 337.316 kb on + strand, within BWI76_RS01660 at 337.333 kb on - strand, within BWI76_RS01660 at 337.362 kb on - strand, within BWI76_RS01660 at 337.365 kb on - strand, within BWI76_RS01660 at 337.629 kb on - strand, within BWI76_RS01660 at 337.650 kb on - strand, within BWI76_RS01660 at 337.717 kb on + strand, within BWI76_RS01660 at 337.826 kb on + strand, within BWI76_RS01660 at 337.870 kb on - strand, within BWI76_RS01660 at 338.149 kb on - strand, within BWI76_RS01660 at 338.334 kb on + strand, within BWI76_RS01660 at 338.609 kb on + strand at 339.333 kb on - strand, within BWI76_RS01665 at 339.344 kb on + strand, within BWI76_RS01665 at 339.487 kb on + strand, within BWI76_RS01665 at 339.493 kb on + strand, within BWI76_RS01665 at 339.493 kb on + strand, within BWI76_RS01665
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 335,918 - BWI76_RS01655 0.20 -3.8 336,033 + BWI76_RS01655 0.33 +1.1 336,115 - BWI76_RS01655 0.42 -3.6 336,115 - BWI76_RS01655 0.42 -4.8 336,135 + BWI76_RS01655 0.44 -2.3 336,143 - BWI76_RS01655 0.45 -2.7 336,447 + BWI76_RS01655 0.77 -2.8 336,447 + BWI76_RS01655 0.77 -4.5 336,455 - BWI76_RS01655 0.78 -2.5 336,621 - +1.0 336,660 + +0.1 336,771 + +0.4 336,909 - +1.1 336,970 - +2.2 337,020 + -1.2 337,284 - BWI76_RS01660 0.23 -0.1 337,316 + BWI76_RS01660 0.25 +1.7 337,333 - BWI76_RS01660 0.26 +2.2 337,362 - BWI76_RS01660 0.28 -0.9 337,365 - BWI76_RS01660 0.28 +0.4 337,629 - BWI76_RS01660 0.44 +0.1 337,650 - BWI76_RS01660 0.46 -0.1 337,717 + BWI76_RS01660 0.50 -0.1 337,826 + BWI76_RS01660 0.57 -0.3 337,870 - BWI76_RS01660 0.59 +1.6 338,149 - BWI76_RS01660 0.77 +0.3 338,334 + BWI76_RS01660 0.88 -0.0 338,609 + +0.5 339,333 - BWI76_RS01665 0.60 +1.6 339,344 + BWI76_RS01665 0.61 +0.9 339,487 + BWI76_RS01665 0.72 -0.3 339,493 + BWI76_RS01665 0.72 +0.9 339,493 + BWI76_RS01665 0.72 -1.1
Or see this region's nucleotide sequence