Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01525 and BWI76_RS01530 are separated by 318 nucleotides BWI76_RS01530 and BWI76_RS01535 overlap by 4 nucleotides BWI76_RS01535 and BWI76_RS01540 are separated by 1 nucleotides BWI76_RS01540 and BWI76_RS01545 overlap by 17 nucleotides
BWI76_RS01525: BWI76_RS01525 - sensor domain-containing diguanylate cyclase, at 310,703 to 312,235
_RS01525
BWI76_RS01530: BWI76_RS01530 - 2-iminoacetate synthase ThiH, at 312,554 to 313,687
_RS01530
BWI76_RS01535: BWI76_RS01535 - thiazole synthase, at 313,684 to 314,454
_RS01535
BWI76_RS01540: BWI76_RS01540 - thiamine biosynthesis protein ThiS, at 314,456 to 314,656
_RS01540
BWI76_RS01545: BWI76_RS01545 - thiamine biosynthesis protein ThiS, at 314,640 to 315,395
_RS01545
Position (kb) 312
313
314 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 311.588 kb on + strand, within BWI76_RS01525 at 311.657 kb on - strand, within BWI76_RS01525 at 311.679 kb on - strand, within BWI76_RS01525 at 311.712 kb on + strand, within BWI76_RS01525 at 311.720 kb on - strand, within BWI76_RS01525 at 311.722 kb on + strand, within BWI76_RS01525 at 311.742 kb on - strand, within BWI76_RS01525 at 311.763 kb on + strand, within BWI76_RS01525 at 311.781 kb on - strand, within BWI76_RS01525 at 311.840 kb on + strand, within BWI76_RS01525 at 312.239 kb on - strand at 312.259 kb on + strand at 312.265 kb on - strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.337 kb on - strand at 312.392 kb on + strand at 312.399 kb on + strand at 312.406 kb on - strand at 312.416 kb on - strand at 312.435 kb on + strand at 312.443 kb on - strand at 312.443 kb on - strand at 312.452 kb on + strand at 312.460 kb on - strand at 312.462 kb on + strand at 312.488 kb on + strand at 312.562 kb on + strand at 312.570 kb on - strand at 312.570 kb on - strand at 312.570 kb on - strand at 312.577 kb on + strand at 312.587 kb on + strand at 312.596 kb on - strand at 312.643 kb on + strand at 312.666 kb on + strand at 312.674 kb on - strand, within BWI76_RS01530 at 312.674 kb on - strand, within BWI76_RS01530 at 312.941 kb on + strand, within BWI76_RS01530 at 313.038 kb on + strand, within BWI76_RS01530 at 313.466 kb on + strand, within BWI76_RS01530 at 313.636 kb on + strand at 313.636 kb on + strand at 313.716 kb on + strand at 313.846 kb on + strand at 313.849 kb on - strand, within BWI76_RS01535 at 313.908 kb on - strand, within BWI76_RS01535 at 314.057 kb on - strand, within BWI76_RS01535 at 314.068 kb on - strand, within BWI76_RS01535 at 314.080 kb on + strand, within BWI76_RS01535 at 314.178 kb on + strand, within BWI76_RS01535 at 314.257 kb on - strand, within BWI76_RS01535 at 314.337 kb on + strand, within BWI76_RS01535 at 314.624 kb on + strand, within BWI76_RS01540 at 314.624 kb on + strand, within BWI76_RS01540
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 311,588 + BWI76_RS01525 0.58 -0.1 311,657 - BWI76_RS01525 0.62 +1.1 311,679 - BWI76_RS01525 0.64 +0.2 311,712 + BWI76_RS01525 0.66 +2.1 311,720 - BWI76_RS01525 0.66 -1.4 311,722 + BWI76_RS01525 0.66 -0.0 311,742 - BWI76_RS01525 0.68 -0.4 311,763 + BWI76_RS01525 0.69 +0.5 311,781 - BWI76_RS01525 0.70 -1.1 311,840 + BWI76_RS01525 0.74 +0.5 312,239 - -2.6 312,259 + -0.3 312,265 - -2.1 312,282 + +1.1 312,282 + +2.1 312,282 + -3.3 312,282 + +0.2 312,282 + -0.5 312,282 + -0.1 312,282 + -1.9 312,282 + +0.7 312,282 + +1.2 312,290 - +0.4 312,290 - -0.6 312,290 - -1.7 312,290 - -0.5 312,290 - -0.2 312,290 - -0.5 312,290 - +0.5 312,337 - -0.8 312,392 + -1.3 312,399 + -0.9 312,406 - -0.3 312,416 - -0.5 312,435 + +1.4 312,443 - +1.2 312,443 - -0.3 312,452 + +1.3 312,460 - +0.1 312,462 + +0.4 312,488 + -0.6 312,562 + +0.1 312,570 - +0.7 312,570 - +0.2 312,570 - +0.7 312,577 + +1.3 312,587 + -2.4 312,596 - +1.1 312,643 + -1.8 312,666 + +1.1 312,674 - BWI76_RS01530 0.11 +0.2 312,674 - BWI76_RS01530 0.11 -1.4 312,941 + BWI76_RS01530 0.34 -2.7 313,038 + BWI76_RS01530 0.43 -0.3 313,466 + BWI76_RS01530 0.80 -2.5 313,636 + +0.2 313,636 + +0.1 313,716 + +0.7 313,846 + +0.1 313,849 - BWI76_RS01535 0.21 +0.7 313,908 - BWI76_RS01535 0.29 -0.5 314,057 - BWI76_RS01535 0.48 -1.7 314,068 - BWI76_RS01535 0.50 +0.8 314,080 + BWI76_RS01535 0.51 -0.5 314,178 + BWI76_RS01535 0.64 +0.1 314,257 - BWI76_RS01535 0.74 +1.9 314,337 + BWI76_RS01535 0.85 -0.8 314,624 + BWI76_RS01540 0.84 -1.1 314,624 + BWI76_RS01540 0.84 -0.6
Or see this region's nucleotide sequence