Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00860 and BWI76_RS00865 overlap by 1 nucleotides BWI76_RS00865 and BWI76_RS00870 overlap by 18 nucleotides BWI76_RS00870 and BWI76_RS00875 are separated by 196 nucleotides BWI76_RS00875 and BWI76_RS00880 are separated by 15 nucleotides
BWI76_RS00860: BWI76_RS00860 - hypothetical protein, at 178,191 to 178,430
_RS00860
BWI76_RS00865: BWI76_RS00865 - DNA-binding transcriptional regulator OxyR, at 178,430 to 179,347
_RS00865
BWI76_RS00870: BWI76_RS00870 - NAD(P)(+) transhydrogenase, at 179,330 to 180,730
_RS00870
BWI76_RS00875: BWI76_RS00875 - DNA-binding transcriptional regulator FabR, at 180,927 to 181,562
_RS00875
BWI76_RS00880: BWI76_RS00880 - membrane protein, at 181,578 to 181,937
_RS00880
Position (kb)
179
180
181 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 178.551 kb on - strand, within BWI76_RS00865 at 178.618 kb on - strand, within BWI76_RS00865 at 178.761 kb on - strand, within BWI76_RS00865 at 178.943 kb on + strand, within BWI76_RS00865 at 179.153 kb on + strand, within BWI76_RS00865 at 179.208 kb on - strand, within BWI76_RS00865 at 179.279 kb on - strand at 180.794 kb on - strand at 180.883 kb on - strand at 180.885 kb on + strand at 180.915 kb on + strand at 180.923 kb on - strand at 181.021 kb on + strand, within BWI76_RS00875 at 181.021 kb on + strand, within BWI76_RS00875 at 181.021 kb on + strand, within BWI76_RS00875 at 181.109 kb on + strand, within BWI76_RS00875 at 181.129 kb on + strand, within BWI76_RS00875 at 181.294 kb on + strand, within BWI76_RS00875 at 181.383 kb on + strand, within BWI76_RS00875 at 181.388 kb on + strand, within BWI76_RS00875 at 181.396 kb on - strand, within BWI76_RS00875 at 181.444 kb on + strand, within BWI76_RS00875 at 181.586 kb on - strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.595 kb on + strand at 181.595 kb on + strand at 181.598 kb on - strand at 181.603 kb on - strand at 181.659 kb on + strand, within BWI76_RS00880 at 181.661 kb on + strand, within BWI76_RS00880 at 181.661 kb on + strand, within BWI76_RS00880
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 178,551 - BWI76_RS00865 0.13 +2.8 178,618 - BWI76_RS00865 0.20 +0.5 178,761 - BWI76_RS00865 0.36 +2.7 178,943 + BWI76_RS00865 0.56 +1.8 179,153 + BWI76_RS00865 0.79 +1.8 179,208 - BWI76_RS00865 0.85 +0.9 179,279 - -1.3 180,794 - +1.1 180,883 - +1.1 180,885 + -0.6 180,915 + -0.9 180,923 - -3.2 181,021 + BWI76_RS00875 0.15 -3.9 181,021 + BWI76_RS00875 0.15 -0.9 181,021 + BWI76_RS00875 0.15 +1.1 181,109 + BWI76_RS00875 0.29 +1.1 181,129 + BWI76_RS00875 0.32 -3.7 181,294 + BWI76_RS00875 0.58 -2.5 181,383 + BWI76_RS00875 0.72 -1.8 181,388 + BWI76_RS00875 0.72 -2.8 181,396 - BWI76_RS00875 0.74 +1.1 181,444 + BWI76_RS00875 0.81 -2.9 181,586 - +0.1 181,590 + +1.5 181,590 + +1.1 181,590 + -0.8 181,590 + +0.6 181,590 + -1.3 181,595 + +0.7 181,595 + +0.7 181,598 - +0.1 181,603 - +0.3 181,659 + BWI76_RS00880 0.23 -0.2 181,661 + BWI76_RS00880 0.23 +0.9 181,661 + BWI76_RS00880 0.23 -0.5
Or see this region's nucleotide sequence