Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00255

Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00250 and BWI76_RS00255 are separated by 7 nucleotidesBWI76_RS00255 and BWI76_RS00260 overlap by 4 nucleotides BWI76_RS00250: BWI76_RS00250 - asparagine synthetase A, at 50,095 to 51,087 _RS00250 BWI76_RS00255: BWI76_RS00255 - hypothetical protein, at 51,095 to 52,543 _RS00255 BWI76_RS00260: BWI76_RS00260 - ATPase RavA, at 52,540 to 54,039 _RS00260 Position (kb) 51 52 53Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 50.111 kb on + strandat 50.158 kb on - strandat 50.227 kb on + strand, within BWI76_RS00250at 50.237 kb on - strand, within BWI76_RS00250at 50.300 kb on - strand, within BWI76_RS00250at 50.335 kb on - strand, within BWI76_RS00250at 50.346 kb on + strand, within BWI76_RS00250at 50.367 kb on + strand, within BWI76_RS00250at 50.387 kb on - strand, within BWI76_RS00250at 50.398 kb on + strand, within BWI76_RS00250at 50.440 kb on - strand, within BWI76_RS00250at 50.445 kb on - strand, within BWI76_RS00250at 50.450 kb on - strand, within BWI76_RS00250at 50.454 kb on - strand, within BWI76_RS00250at 50.604 kb on - strand, within BWI76_RS00250at 50.839 kb on - strand, within BWI76_RS00250at 50.936 kb on - strand, within BWI76_RS00250at 50.975 kb on + strand, within BWI76_RS00250at 50.975 kb on + strand, within BWI76_RS00250at 51.015 kb on + strandat 51.301 kb on + strand, within BWI76_RS00255at 51.353 kb on - strand, within BWI76_RS00255at 51.516 kb on + strand, within BWI76_RS00255at 51.543 kb on - strand, within BWI76_RS00255at 51.672 kb on + strand, within BWI76_RS00255at 51.786 kb on - strand, within BWI76_RS00255at 51.893 kb on - strand, within BWI76_RS00255at 51.912 kb on + strand, within BWI76_RS00255at 52.043 kb on - strand, within BWI76_RS00255at 52.043 kb on - strand, within BWI76_RS00255at 52.054 kb on + strand, within BWI76_RS00255at 52.064 kb on + strand, within BWI76_RS00255at 52.114 kb on + strand, within BWI76_RS00255at 52.183 kb on + strand, within BWI76_RS00255at 52.191 kb on - strand, within BWI76_RS00255at 52.191 kb on - strand, within BWI76_RS00255at 52.199 kb on - strand, within BWI76_RS00255at 52.389 kb on - strand, within BWI76_RS00255at 52.504 kb on - strandat 52.599 kb on + strandat 52.778 kb on + strand, within BWI76_RS00260at 52.782 kb on + strand, within BWI76_RS00260at 52.782 kb on + strand, within BWI76_RS00260at 52.867 kb on - strand, within BWI76_RS00260at 53.097 kb on - strand, within BWI76_RS00260at 53.099 kb on - strand, within BWI76_RS00260at 53.149 kb on - strand, within BWI76_RS00260at 53.149 kb on - strand, within BWI76_RS00260at 53.149 kb on - strandat 53.257 kb on - strand, within BWI76_RS00260at 53.362 kb on + strand, within BWI76_RS00260at 53.417 kb on - strand, within BWI76_RS00260at 53.417 kb on - strand, within BWI76_RS00260at 53.441 kb on + strand, within BWI76_RS00260at 53.457 kb on - strand, within BWI76_RS00260

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
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50,111 + +0.0
50,158 - -0.0
50,227 + BWI76_RS00250 0.13 +0.9
50,237 - BWI76_RS00250 0.14 -0.6
50,300 - BWI76_RS00250 0.21 +0.4
50,335 - BWI76_RS00250 0.24 +1.0
50,346 + BWI76_RS00250 0.25 +1.1
50,367 + BWI76_RS00250 0.27 +2.5
50,387 - BWI76_RS00250 0.29 +1.1
50,398 + BWI76_RS00250 0.31 +0.0
50,440 - BWI76_RS00250 0.35 +0.6
50,445 - BWI76_RS00250 0.35 -2.3
50,450 - BWI76_RS00250 0.36 +0.0
50,454 - BWI76_RS00250 0.36 +0.1
50,604 - BWI76_RS00250 0.51 -0.6
50,839 - BWI76_RS00250 0.75 -0.0
50,936 - BWI76_RS00250 0.85 +1.5
50,975 + BWI76_RS00250 0.89 +1.1
50,975 + BWI76_RS00250 0.89 -0.6
51,015 + +0.0
51,301 + BWI76_RS00255 0.14 -0.7
51,353 - BWI76_RS00255 0.18 +1.4
51,516 + BWI76_RS00255 0.29 +0.2
51,543 - BWI76_RS00255 0.31 -0.4
51,672 + BWI76_RS00255 0.40 +0.1
51,786 - BWI76_RS00255 0.48 +2.0
51,893 - BWI76_RS00255 0.55 +0.1
51,912 + BWI76_RS00255 0.56 +2.4
52,043 - BWI76_RS00255 0.65 +1.0
52,043 - BWI76_RS00255 0.65 +0.3
52,054 + BWI76_RS00255 0.66 +0.3
52,064 + BWI76_RS00255 0.67 -3.1
52,114 + BWI76_RS00255 0.70 +1.3
52,183 + BWI76_RS00255 0.75 +1.0
52,191 - BWI76_RS00255 0.76 +0.2
52,191 - BWI76_RS00255 0.76 +0.7
52,199 - BWI76_RS00255 0.76 -0.2
52,389 - BWI76_RS00255 0.89 +0.7
52,504 - +1.1
52,599 + +0.1
52,778 + BWI76_RS00260 0.16 +0.8
52,782 + BWI76_RS00260 0.16 +1.1
52,782 + BWI76_RS00260 0.16 -1.9
52,867 - BWI76_RS00260 0.22 +0.7
53,097 - BWI76_RS00260 0.37 -0.5
53,099 - BWI76_RS00260 0.37 +3.6
53,149 - BWI76_RS00260 0.41 -0.1
53,149 - BWI76_RS00260 0.41 +1.4
53,149 - +0.1
53,257 - BWI76_RS00260 0.48 +1.2
53,362 + BWI76_RS00260 0.55 +0.2
53,417 - BWI76_RS00260 0.58 +0.4
53,417 - BWI76_RS00260 0.58 +0.1
53,441 + BWI76_RS00260 0.60 -0.2
53,457 - BWI76_RS00260 0.61 +0.2

Or see this region's nucleotide sequence