Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26220 and BWI76_RS26225 are separated by 358 nucleotides BWI76_RS26225 and BWI76_RS26230 overlap by 4 nucleotides
BWI76_RS26220: BWI76_RS26220 - aspartate-semialdehyde dehydrogenase, at 5,426,671 to 5,427,777
_RS26220
BWI76_RS26225: BWI76_RS26225 - DNA processing protein DprA, at 5,428,136 to 5,429,341
_RS26225
BWI76_RS26230: BWI76_RS26230 - ATP-dependent DNA helicase, at 5,429,338 to 5,431,437
_RS26230
Position (kb)
5428
5429
5430 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5427.801 kb on - strand at 5427.826 kb on - strand at 5427.836 kb on - strand at 5428.144 kb on + strand at 5428.152 kb on - strand at 5428.152 kb on - strand at 5428.184 kb on + strand at 5428.200 kb on - strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.236 kb on - strand at 5428.388 kb on + strand, within BWI76_RS26225 at 5428.407 kb on + strand, within BWI76_RS26225 at 5428.530 kb on - strand, within BWI76_RS26225 at 5428.569 kb on - strand, within BWI76_RS26225 at 5428.714 kb on - strand, within BWI76_RS26225 at 5428.822 kb on + strand, within BWI76_RS26225 at 5428.822 kb on + strand, within BWI76_RS26225 at 5428.944 kb on - strand, within BWI76_RS26225 at 5429.056 kb on + strand, within BWI76_RS26225 at 5429.056 kb on + strand, within BWI76_RS26225 at 5429.062 kb on + strand, within BWI76_RS26225 at 5429.098 kb on + strand, within BWI76_RS26225 at 5429.179 kb on + strand, within BWI76_RS26225 at 5429.276 kb on + strand at 5429.284 kb on - strand at 5429.479 kb on - strand at 5429.644 kb on - strand, within BWI76_RS26230 at 5430.001 kb on - strand, within BWI76_RS26230 at 5430.017 kb on + strand, within BWI76_RS26230 at 5430.031 kb on - strand, within BWI76_RS26230 at 5430.063 kb on + strand, within BWI76_RS26230 at 5430.071 kb on + strand, within BWI76_RS26230 at 5430.071 kb on + strand, within BWI76_RS26230 at 5430.079 kb on - strand, within BWI76_RS26230 at 5430.079 kb on - strand, within BWI76_RS26230 at 5430.159 kb on - strand, within BWI76_RS26230 at 5430.247 kb on + strand, within BWI76_RS26230 at 5430.249 kb on + strand, within BWI76_RS26230 at 5430.255 kb on - strand, within BWI76_RS26230 at 5430.287 kb on - strand, within BWI76_RS26230 at 5430.315 kb on + strand, within BWI76_RS26230
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 5,427,801 - -0.1 5,427,826 - +1.1 5,427,836 - +0.0 5,428,144 + -1.2 5,428,152 - -0.1 5,428,152 - -0.1 5,428,184 + +1.1 5,428,200 - +0.1 5,428,206 + -1.5 5,428,206 + +0.1 5,428,206 + -1.1 5,428,206 + -2.1 5,428,206 + +0.5 5,428,206 + +0.9 5,428,206 + +0.1 5,428,206 + -1.7 5,428,214 - -1.4 5,428,214 - +0.5 5,428,214 - -1.9 5,428,214 - -0.5 5,428,214 - -1.9 5,428,214 - +0.5 5,428,228 + -0.9 5,428,228 + +0.5 5,428,228 + -0.2 5,428,228 + +0.1 5,428,228 + +0.5 5,428,228 + +1.3 5,428,236 - -0.5 5,428,388 + BWI76_RS26225 0.21 -0.6 5,428,407 + BWI76_RS26225 0.22 +0.0 5,428,530 - BWI76_RS26225 0.33 +0.3 5,428,569 - BWI76_RS26225 0.36 +0.7 5,428,714 - BWI76_RS26225 0.48 +0.1 5,428,822 + BWI76_RS26225 0.57 +2.6 5,428,822 + BWI76_RS26225 0.57 +0.6 5,428,944 - BWI76_RS26225 0.67 -1.3 5,429,056 + BWI76_RS26225 0.76 +1.1 5,429,056 + BWI76_RS26225 0.76 -0.0 5,429,062 + BWI76_RS26225 0.77 +1.9 5,429,098 + BWI76_RS26225 0.80 +0.4 5,429,179 + BWI76_RS26225 0.86 -0.0 5,429,276 + +0.5 5,429,284 - -0.5 5,429,479 - +0.5 5,429,644 - BWI76_RS26230 0.15 +0.7 5,430,001 - BWI76_RS26230 0.32 -0.6 5,430,017 + BWI76_RS26230 0.32 -0.2 5,430,031 - BWI76_RS26230 0.33 +0.5 5,430,063 + BWI76_RS26230 0.35 +2.2 5,430,071 + BWI76_RS26230 0.35 +2.9 5,430,071 + BWI76_RS26230 0.35 +0.2 5,430,079 - BWI76_RS26230 0.35 -0.9 5,430,079 - BWI76_RS26230 0.35 -0.1 5,430,159 - BWI76_RS26230 0.39 -0.4 5,430,247 + BWI76_RS26230 0.43 -0.8 5,430,249 + BWI76_RS26230 0.43 +1.5 5,430,255 - BWI76_RS26230 0.44 +0.6 5,430,287 - BWI76_RS26230 0.45 -0.2 5,430,315 + BWI76_RS26230 0.47 +0.8
Or see this region's nucleotide sequence