Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26215

Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26210 and BWI76_RS26215 overlap by 4 nucleotidesBWI76_RS26215 and BWI76_RS26220 are separated by 247 nucleotides BWI76_RS26210: BWI76_RS26210 - glycogen debranching enzyme, at 5,422,264 to 5,424,240 _RS26210 BWI76_RS26215: BWI76_RS26215 - glycogen-branching enzyme, at 5,424,237 to 5,426,423 _RS26215 BWI76_RS26220: BWI76_RS26220 - aspartate-semialdehyde dehydrogenase, at 5,426,671 to 5,427,777 _RS26220 Position (kb) 5424 5425 5426 5427Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5423.454 kb on - strand, within BWI76_RS26210at 5423.763 kb on - strand, within BWI76_RS26210at 5423.770 kb on - strand, within BWI76_RS26210at 5423.802 kb on - strand, within BWI76_RS26210at 5423.915 kb on + strand, within BWI76_RS26210at 5423.978 kb on - strand, within BWI76_RS26210at 5424.138 kb on + strandat 5424.183 kb on - strandat 5424.345 kb on - strandat 5424.504 kb on + strand, within BWI76_RS26215at 5424.604 kb on - strand, within BWI76_RS26215at 5424.652 kb on + strand, within BWI76_RS26215at 5424.675 kb on + strand, within BWI76_RS26215at 5424.786 kb on + strand, within BWI76_RS26215at 5425.243 kb on + strand, within BWI76_RS26215at 5425.243 kb on + strand, within BWI76_RS26215at 5425.251 kb on - strand, within BWI76_RS26215at 5425.317 kb on + strand, within BWI76_RS26215at 5425.356 kb on - strand, within BWI76_RS26215at 5425.578 kb on + strand, within BWI76_RS26215at 5425.943 kb on + strand, within BWI76_RS26215at 5426.125 kb on - strand, within BWI76_RS26215at 5426.334 kb on + strandat 5426.413 kb on + strandat 5426.489 kb on - strandat 5426.501 kb on - strandat 5426.513 kb on - strandat 5426.535 kb on - strandat 5426.572 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
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5,423,454 - BWI76_RS26210 0.60 +1.4
5,423,763 - BWI76_RS26210 0.76 +0.8
5,423,770 - BWI76_RS26210 0.76 -1.8
5,423,802 - BWI76_RS26210 0.78 -1.5
5,423,915 + BWI76_RS26210 0.84 -0.8
5,423,978 - BWI76_RS26210 0.87 -1.3
5,424,138 + +1.8
5,424,183 - -0.8
5,424,345 - +3.5
5,424,504 + BWI76_RS26215 0.12 +0.6
5,424,604 - BWI76_RS26215 0.17 -1.5
5,424,652 + BWI76_RS26215 0.19 -0.5
5,424,675 + BWI76_RS26215 0.20 -1.9
5,424,786 + BWI76_RS26215 0.25 -0.5
5,425,243 + BWI76_RS26215 0.46 +0.5
5,425,243 + BWI76_RS26215 0.46 +0.1
5,425,251 - BWI76_RS26215 0.46 +1.3
5,425,317 + BWI76_RS26215 0.49 -0.9
5,425,356 - BWI76_RS26215 0.51 -1.3
5,425,578 + BWI76_RS26215 0.61 +1.1
5,425,943 + BWI76_RS26215 0.78 +0.4
5,426,125 - BWI76_RS26215 0.86 -0.3
5,426,334 + -0.0
5,426,413 + +1.0
5,426,489 - -3.3
5,426,501 - -0.3
5,426,513 - +1.1
5,426,535 - -0.2
5,426,572 + +0.6

Or see this region's nucleotide sequence