Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25985 and BWI76_RS25990 are separated by 157 nucleotides BWI76_RS25990 and BWI76_RS25995 are separated by 51 nucleotides BWI76_RS25995 and BWI76_RS26000 overlap by 11 nucleotides
BWI76_RS25980: BWI76_RS25980 - hypothetical protein, at 5,370,386 to 5,370,790
_RS25980
BWI76_RS25985: BWI76_RS25985 - hypothetical protein, at 5,370,750 to 5,370,937
_RS25985
BWI76_RS25990: BWI76_RS25990 - cAMP-activated global transcriptional regulator CRP, at 5,371,095 to 5,371,727
_RS25990
BWI76_RS25995: BWI76_RS25995 - membrane protein, at 5,371,779 to 5,373,857
_RS25995
BWI76_RS26000: BWI76_RS26000 - aspartate aminotransferase family protein, at 5,373,847 to 5,375,067
_RS26000
Position (kb) 5371
5372
5373
5374 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5370.968 kb on + strand at 5370.971 kb on - strand at 5370.992 kb on + strand at 5371.814 kb on - strand at 5371.890 kb on + strand at 5372.219 kb on + strand, within BWI76_RS25995 at 5372.276 kb on - strand, within BWI76_RS25995 at 5372.310 kb on + strand, within BWI76_RS25995 at 5372.400 kb on + strand, within BWI76_RS25995 at 5372.530 kb on - strand, within BWI76_RS25995 at 5372.598 kb on - strand, within BWI76_RS25995 at 5372.885 kb on + strand, within BWI76_RS25995 at 5372.890 kb on + strand, within BWI76_RS25995 at 5372.890 kb on + strand, within BWI76_RS25995 at 5372.921 kb on - strand, within BWI76_RS25995 at 5372.936 kb on + strand, within BWI76_RS25995 at 5372.944 kb on - strand, within BWI76_RS25995 at 5373.073 kb on - strand, within BWI76_RS25995 at 5373.086 kb on - strand, within BWI76_RS25995 at 5373.093 kb on - strand, within BWI76_RS25995 at 5373.105 kb on - strand, within BWI76_RS25995 at 5373.110 kb on - strand, within BWI76_RS25995 at 5373.159 kb on - strand, within BWI76_RS25995 at 5373.174 kb on - strand, within BWI76_RS25995 at 5373.183 kb on + strand, within BWI76_RS25995 at 5373.372 kb on - strand, within BWI76_RS25995 at 5373.545 kb on + strand, within BWI76_RS25995 at 5373.553 kb on - strand, within BWI76_RS25995 at 5373.569 kb on - strand, within BWI76_RS25995 at 5373.618 kb on - strand, within BWI76_RS25995 at 5373.930 kb on + strand at 5373.948 kb on - strand at 5374.067 kb on + strand, within BWI76_RS26000 at 5374.262 kb on + strand, within BWI76_RS26000 at 5374.302 kb on + strand, within BWI76_RS26000 at 5374.312 kb on + strand, within BWI76_RS26000 at 5374.312 kb on + strand, within BWI76_RS26000 at 5374.315 kb on - strand, within BWI76_RS26000 at 5374.396 kb on + strand, within BWI76_RS26000 at 5374.418 kb on - strand, within BWI76_RS26000 at 5374.475 kb on - strand, within BWI76_RS26000 at 5374.649 kb on + strand, within BWI76_RS26000 at 5374.823 kb on - strand, within BWI76_RS26000
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 5,370,968 + -1.0 5,370,971 - +0.1 5,370,992 + -0.1 5,371,814 - +0.7 5,371,890 + +0.6 5,372,219 + BWI76_RS25995 0.21 +0.2 5,372,276 - BWI76_RS25995 0.24 +1.2 5,372,310 + BWI76_RS25995 0.26 -1.0 5,372,400 + BWI76_RS25995 0.30 +0.8 5,372,530 - BWI76_RS25995 0.36 -0.1 5,372,598 - BWI76_RS25995 0.39 +2.3 5,372,885 + BWI76_RS25995 0.53 +0.9 5,372,890 + BWI76_RS25995 0.53 +1.7 5,372,890 + BWI76_RS25995 0.53 -0.9 5,372,921 - BWI76_RS25995 0.55 +3.2 5,372,936 + BWI76_RS25995 0.56 +1.0 5,372,944 - BWI76_RS25995 0.56 +1.9 5,373,073 - BWI76_RS25995 0.62 -1.8 5,373,086 - BWI76_RS25995 0.63 +0.4 5,373,093 - BWI76_RS25995 0.63 +0.5 5,373,105 - BWI76_RS25995 0.64 -0.3 5,373,110 - BWI76_RS25995 0.64 +0.7 5,373,159 - BWI76_RS25995 0.66 +1.0 5,373,174 - BWI76_RS25995 0.67 +1.1 5,373,183 + BWI76_RS25995 0.68 +0.2 5,373,372 - BWI76_RS25995 0.77 -0.6 5,373,545 + BWI76_RS25995 0.85 +0.9 5,373,553 - BWI76_RS25995 0.85 +1.6 5,373,569 - BWI76_RS25995 0.86 +0.4 5,373,618 - BWI76_RS25995 0.88 -1.2 5,373,930 + +1.1 5,373,948 - -4.2 5,374,067 + BWI76_RS26000 0.18 -4.5 5,374,262 + BWI76_RS26000 0.34 -4.0 5,374,302 + BWI76_RS26000 0.37 -3.5 5,374,312 + BWI76_RS26000 0.38 -2.2 5,374,312 + BWI76_RS26000 0.38 -3.0 5,374,315 - BWI76_RS26000 0.38 -3.9 5,374,396 + BWI76_RS26000 0.45 -4.0 5,374,418 - BWI76_RS26000 0.47 -1.4 5,374,475 - BWI76_RS26000 0.51 -3.5 5,374,649 + BWI76_RS26000 0.66 +0.1 5,374,823 - BWI76_RS26000 0.80 -2.9
Or see this region's nucleotide sequence