Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21540 and BWI76_RS21545 are separated by 65 nucleotides BWI76_RS21545 and BWI76_RS21550 overlap by 36 nucleotides BWI76_RS21550 and BWI76_RS21555 are separated by 13 nucleotides
BWI76_RS21540: BWI76_RS21540 - membrane protein, at 4,510,017 to 4,511,306
_RS21540
BWI76_RS21545: BWI76_RS21545 - nucleotide exchange factor GrpE, at 4,511,372 to 4,511,962
_RS21545
BWI76_RS21550: BWI76_RS21550 - inorganic polyphosphate kinase, at 4,511,927 to 4,512,073
_RS21550
BWI76_RS21555: BWI76_RS21555 - NAD(+) kinase, at 4,512,087 to 4,512,965
_RS21555
Position (kb) 4511
4512 Strain fitness (log2 ratio) -2
-1
0
1 at 4510.602 kb on + strand, within BWI76_RS21540 at 4510.833 kb on - strand, within BWI76_RS21540 at 4510.961 kb on + strand, within BWI76_RS21540 at 4510.969 kb on - strand, within BWI76_RS21540 at 4511.210 kb on + strand at 4511.218 kb on - strand at 4511.232 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 4,510,602 + BWI76_RS21540 0.45 +1.0 4,510,833 - BWI76_RS21540 0.63 -2.0 4,510,961 + BWI76_RS21540 0.73 +1.0 4,510,969 - BWI76_RS21540 0.74 +0.5 4,511,210 + -0.4 4,511,218 - -0.3 4,511,232 + +0.7
Or see this region's nucleotide sequence