Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21305 and BWI76_RS21310 are separated by 11 nucleotides BWI76_RS21310 and BWI76_RS21315 are separated by 11 nucleotides BWI76_RS21315 and BWI76_RS21320 overlap by 4 nucleotides BWI76_RS21320 and BWI76_RS21325 overlap by 57 nucleotides BWI76_RS21325 and BWI76_RS21330 are separated by 50 nucleotides
BWI76_RS21305: BWI76_RS21305 - pyridoxine 5'-phosphate synthase, at 4,461,706 to 4,462,437
_RS21305
BWI76_RS21310: BWI76_RS21310 - DNA repair protein RecO, at 4,462,449 to 4,463,186
_RS21310
BWI76_RS21315: BWI76_RS21315 - GTPase Era, at 4,463,198 to 4,464,103
_RS21315
BWI76_RS21320: BWI76_RS21320 - ribonuclease 3, at 4,464,100 to 4,464,780
_RS21320
BWI76_RS21325: BWI76_RS21325 - hypothetical protein, at 4,464,724 to 4,464,954
_RS21325
BWI76_RS21330: BWI76_RS21330 - S26 family signal peptidase, at 4,465,005 to 4,465,979
_RS21330
Position (kb) 4463
4464
4465 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4462.497 kb on - strand at 4463.024 kb on - strand, within BWI76_RS21310 at 4464.783 kb on - strand, within BWI76_RS21325 at 4464.922 kb on + strand, within BWI76_RS21325 at 4464.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 4,462,497 - +0.4 4,463,024 - BWI76_RS21310 0.78 -0.0 4,464,783 - BWI76_RS21325 0.26 -0.7 4,464,922 + BWI76_RS21325 0.86 -2.4 4,464,992 - -3.4
Or see this region's nucleotide sequence