Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02305 and BWI76_RS02310 are separated by 67 nucleotides BWI76_RS02310 and BWI76_RS02315 are separated by 35 nucleotides BWI76_RS02315 and BWI76_RS02320 overlap by 25 nucleotides BWI76_RS02320 and BWI76_RS02325 are separated by 94 nucleotides
BWI76_RS02305: BWI76_RS02305 - hypothetical protein, at 473,077 to 475,164
_RS02305
BWI76_RS02310: BWI76_RS02310 - transcriptional regulator, at 475,232 to 475,807
_RS02310
BWI76_RS02315: BWI76_RS02315 - protein-disulfide reductase DsbD, at 475,843 to 477,600
_RS02315
BWI76_RS02320: BWI76_RS02320 - divalent ion tolerance protein CutA, at 477,576 to 477,899
_RS02320
BWI76_RS02325: BWI76_RS02325 - anaerobic C4-dicarboxylate transporter, at 477,994 to 479,295
_RS02325
Position (kb) 475
476
477
478 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 474.890 kb on + strand, within BWI76_RS02305 at 474.996 kb on - strand at 475.014 kb on + strand at 475.121 kb on + strand at 475.127 kb on - strand at 475.146 kb on + strand at 475.172 kb on - strand at 475.172 kb on - strand at 475.251 kb on + strand at 475.251 kb on + strand at 475.328 kb on - strand, within BWI76_RS02310 at 475.346 kb on + strand, within BWI76_RS02310 at 475.540 kb on + strand, within BWI76_RS02310 at 475.548 kb on - strand, within BWI76_RS02310 at 475.592 kb on - strand, within BWI76_RS02310 at 475.688 kb on - strand, within BWI76_RS02310 at 475.699 kb on + strand, within BWI76_RS02310 at 475.854 kb on - strand at 475.906 kb on - strand at 475.973 kb on - strand at 476.037 kb on + strand, within BWI76_RS02315 at 476.045 kb on - strand, within BWI76_RS02315 at 476.078 kb on - strand, within BWI76_RS02315 at 476.263 kb on - strand, within BWI76_RS02315 at 476.270 kb on + strand, within BWI76_RS02315 at 476.492 kb on + strand, within BWI76_RS02315 at 476.586 kb on + strand, within BWI76_RS02315 at 476.686 kb on + strand, within BWI76_RS02315 at 476.694 kb on - strand, within BWI76_RS02315 at 476.786 kb on - strand, within BWI76_RS02315 at 476.878 kb on + strand, within BWI76_RS02315 at 477.006 kb on - strand, within BWI76_RS02315 at 477.252 kb on + strand, within BWI76_RS02315 at 477.353 kb on - strand, within BWI76_RS02315 at 477.423 kb on + strand, within BWI76_RS02315 at 477.453 kb on + strand at 477.461 kb on - strand at 477.573 kb on - strand at 477.681 kb on + strand, within BWI76_RS02320 at 477.793 kb on - strand, within BWI76_RS02320 at 477.837 kb on + strand, within BWI76_RS02320 at 477.844 kb on - strand, within BWI76_RS02320 at 477.860 kb on + strand, within BWI76_RS02320 at 477.868 kb on - strand at 477.881 kb on - strand at 477.929 kb on + strand at 477.972 kb on - strand at 478.046 kb on - strand at 478.060 kb on + strand at 478.098 kb on + strand at 478.201 kb on - strand, within BWI76_RS02325 at 478.211 kb on - strand, within BWI76_RS02325 at 478.336 kb on + strand, within BWI76_RS02325 at 478.346 kb on + strand, within BWI76_RS02325 at 478.472 kb on + strand, within BWI76_RS02325 at 478.480 kb on - strand, within BWI76_RS02325 at 478.538 kb on - strand, within BWI76_RS02325 at 478.543 kb on + strand, within BWI76_RS02325 at 478.548 kb on + strand, within BWI76_RS02325 at 478.556 kb on - strand, within BWI76_RS02325
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 474,890 + BWI76_RS02305 0.87 -0.2 474,996 - -3.4 475,014 + -0.5 475,121 + +1.1 475,127 - +1.1 475,146 + +2.4 475,172 - -0.1 475,172 - -2.0 475,251 + +1.2 475,251 + +1.4 475,328 - BWI76_RS02310 0.17 +1.1 475,346 + BWI76_RS02310 0.20 -1.5 475,540 + BWI76_RS02310 0.53 -0.6 475,548 - BWI76_RS02310 0.55 -2.5 475,592 - BWI76_RS02310 0.62 +0.5 475,688 - BWI76_RS02310 0.79 -1.0 475,699 + BWI76_RS02310 0.81 +1.3 475,854 - +1.7 475,906 - -1.0 475,973 - -0.8 476,037 + BWI76_RS02315 0.11 -2.5 476,045 - BWI76_RS02315 0.11 +0.3 476,078 - BWI76_RS02315 0.13 +0.3 476,263 - BWI76_RS02315 0.24 -2.5 476,270 + BWI76_RS02315 0.24 +0.8 476,492 + BWI76_RS02315 0.37 -0.7 476,586 + BWI76_RS02315 0.42 -3.1 476,686 + BWI76_RS02315 0.48 -0.4 476,694 - BWI76_RS02315 0.48 -1.5 476,786 - BWI76_RS02315 0.54 -2.7 476,878 + BWI76_RS02315 0.59 -0.8 477,006 - BWI76_RS02315 0.66 -0.1 477,252 + BWI76_RS02315 0.80 +0.4 477,353 - BWI76_RS02315 0.86 -0.8 477,423 + BWI76_RS02315 0.90 -3.9 477,453 + -3.0 477,461 - +0.5 477,573 - -0.6 477,681 + BWI76_RS02320 0.32 -2.3 477,793 - BWI76_RS02320 0.67 +0.0 477,837 + BWI76_RS02320 0.81 +0.4 477,844 - BWI76_RS02320 0.83 -1.2 477,860 + BWI76_RS02320 0.88 -0.5 477,868 - +1.1 477,881 - +0.5 477,929 + +0.1 477,972 - +1.4 478,046 - +0.6 478,060 + -0.2 478,098 + +0.8 478,201 - BWI76_RS02325 0.16 -0.0 478,211 - BWI76_RS02325 0.17 +0.7 478,336 + BWI76_RS02325 0.26 +0.9 478,346 + BWI76_RS02325 0.27 +0.7 478,472 + BWI76_RS02325 0.37 -0.2 478,480 - BWI76_RS02325 0.37 +0.3 478,538 - BWI76_RS02325 0.42 +0.3 478,543 + BWI76_RS02325 0.42 +1.8 478,548 + BWI76_RS02325 0.43 -1.9 478,556 - BWI76_RS02325 0.43 +1.1
Or see this region's nucleotide sequence