Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01740 and BWI76_RS01745 are separated by 9 nucleotides BWI76_RS01745 and BWI76_RS01750 are separated by 70 nucleotides BWI76_RS01750 and BWI76_RS01755 are separated by 348 nucleotides
BWI76_RS01740: BWI76_RS01740 - PTS enzyme IIAB, mannose-specific, at 356,176 to 356,583
_RS01740
BWI76_RS01745: BWI76_RS01745 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) <small>(from data)</small>, at 356,593 to 357,396
_RS01745
BWI76_RS01750: BWI76_RS01750 - Cro/Cl family transcriptional regulator, at 357,467 to 358,414
_RS01750
BWI76_RS01755: BWI76_RS01755 - 23S rRNA pseudouridine synthase F, at 358,763 to 359,635
_RS01755
Position (kb) 357
358
359 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 356.500 kb on - strand, within BWI76_RS01740 at 356.509 kb on + strand, within BWI76_RS01740 at 356.514 kb on + strand, within BWI76_RS01740 at 356.517 kb on - strand, within BWI76_RS01740 at 356.536 kb on - strand, within BWI76_RS01740 at 356.545 kb on + strand at 356.545 kb on + strand at 356.575 kb on - strand at 356.767 kb on + strand, within BWI76_RS01745 at 356.791 kb on - strand, within BWI76_RS01745 at 356.798 kb on - strand, within BWI76_RS01745 at 356.857 kb on - strand, within BWI76_RS01745 at 356.883 kb on + strand, within BWI76_RS01745 at 356.883 kb on + strand, within BWI76_RS01745 at 356.883 kb on + strand, within BWI76_RS01745 at 356.891 kb on - strand, within BWI76_RS01745 at 356.901 kb on - strand, within BWI76_RS01745 at 356.913 kb on + strand, within BWI76_RS01745 at 356.923 kb on + strand, within BWI76_RS01745 at 356.931 kb on - strand, within BWI76_RS01745 at 356.948 kb on - strand, within BWI76_RS01745 at 356.973 kb on + strand, within BWI76_RS01745 at 356.986 kb on + strand, within BWI76_RS01745 at 356.986 kb on + strand, within BWI76_RS01745 at 356.986 kb on + strand, within BWI76_RS01745 at 356.986 kb on - strand, within BWI76_RS01745 at 356.988 kb on + strand, within BWI76_RS01745 at 356.988 kb on + strand, within BWI76_RS01745 at 356.988 kb on + strand, within BWI76_RS01745 at 356.988 kb on + strand, within BWI76_RS01745 at 356.994 kb on - strand, within BWI76_RS01745 at 356.996 kb on - strand at 356.996 kb on - strand, within BWI76_RS01745 at 356.996 kb on - strand, within BWI76_RS01745 at 356.996 kb on - strand, within BWI76_RS01745 at 356.996 kb on - strand, within BWI76_RS01745 at 356.996 kb on - strand, within BWI76_RS01745 at 357.019 kb on - strand, within BWI76_RS01745 at 357.032 kb on + strand, within BWI76_RS01745 at 357.072 kb on + strand, within BWI76_RS01745 at 357.073 kb on + strand, within BWI76_RS01745 at 357.080 kb on - strand, within BWI76_RS01745 at 357.104 kb on - strand, within BWI76_RS01745 at 357.104 kb on - strand, within BWI76_RS01745 at 357.127 kb on + strand, within BWI76_RS01745 at 357.127 kb on - strand, within BWI76_RS01745 at 357.132 kb on + strand, within BWI76_RS01745 at 357.135 kb on - strand, within BWI76_RS01745 at 357.137 kb on + strand, within BWI76_RS01745 at 357.139 kb on - strand, within BWI76_RS01745 at 357.140 kb on - strand, within BWI76_RS01745 at 357.145 kb on - strand, within BWI76_RS01745 at 357.148 kb on - strand, within BWI76_RS01745 at 357.153 kb on - strand at 357.160 kb on - strand, within BWI76_RS01745 at 357.169 kb on - strand, within BWI76_RS01745 at 357.171 kb on - strand at 357.182 kb on - strand, within BWI76_RS01745 at 357.199 kb on - strand, within BWI76_RS01745 at 357.208 kb on + strand, within BWI76_RS01745 at 357.209 kb on - strand at 357.222 kb on - strand, within BWI76_RS01745 at 357.235 kb on - strand, within BWI76_RS01745 at 357.245 kb on + strand, within BWI76_RS01745 at 357.245 kb on + strand, within BWI76_RS01745 at 357.258 kb on - strand, within BWI76_RS01745 at 357.292 kb on - strand, within BWI76_RS01745 at 357.313 kb on + strand, within BWI76_RS01745 at 357.313 kb on - strand, within BWI76_RS01745 at 357.321 kb on - strand at 357.416 kb on + strand at 357.424 kb on - strand at 357.434 kb on + strand at 357.434 kb on + strand at 357.434 kb on + strand at 357.434 kb on + strand at 357.434 kb on + strand at 357.434 kb on + strand at 357.434 kb on + strand at 357.437 kb on - strand at 357.439 kb on - strand at 357.442 kb on - strand at 357.442 kb on - strand at 357.442 kb on - strand at 357.442 kb on - strand at 357.442 kb on - strand at 357.442 kb on - strand at 357.474 kb on + strand at 357.481 kb on + strand at 357.481 kb on + strand at 357.481 kb on + strand at 357.481 kb on + strand at 357.481 kb on + strand at 357.481 kb on + strand at 357.481 kb on + strand at 357.481 kb on + strand at 357.481 kb on + strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.513 kb on - strand at 357.513 kb on - strand at 357.518 kb on - strand at 357.525 kb on + strand at 357.527 kb on + strand at 357.527 kb on + strand at 357.527 kb on + strand at 357.533 kb on - strand at 357.533 kb on - strand at 357.533 kb on - strand at 357.534 kb on + strand at 357.534 kb on + strand at 357.535 kb on - strand at 357.535 kb on - strand at 357.535 kb on - strand at 357.542 kb on - strand at 357.551 kb on + strand at 357.551 kb on + strand at 357.576 kb on - strand, within BWI76_RS01750 at 357.577 kb on + strand, within BWI76_RS01750 at 357.577 kb on + strand, within BWI76_RS01750 at 357.577 kb on + strand, within BWI76_RS01750 at 357.577 kb on + strand, within BWI76_RS01750 at 357.577 kb on + strand, within BWI76_RS01750 at 357.579 kb on + strand, within BWI76_RS01750 at 357.585 kb on - strand, within BWI76_RS01750 at 357.585 kb on - strand, within BWI76_RS01750 at 357.587 kb on - strand, within BWI76_RS01750 at 357.594 kb on + strand, within BWI76_RS01750 at 357.599 kb on + strand, within BWI76_RS01750 at 357.602 kb on - strand, within BWI76_RS01750 at 357.602 kb on - strand, within BWI76_RS01750 at 357.608 kb on - strand, within BWI76_RS01750 at 357.610 kb on + strand, within BWI76_RS01750 at 357.618 kb on - strand, within BWI76_RS01750 at 357.618 kb on - strand, within BWI76_RS01750 at 357.618 kb on - strand, within BWI76_RS01750 at 357.618 kb on - strand, within BWI76_RS01750 at 357.644 kb on + strand, within BWI76_RS01750 at 357.645 kb on - strand, within BWI76_RS01750 at 357.648 kb on + strand, within BWI76_RS01750 at 357.656 kb on - strand, within BWI76_RS01750 at 357.656 kb on - strand, within BWI76_RS01750 at 357.656 kb on - strand, within BWI76_RS01750 at 357.663 kb on + strand, within BWI76_RS01750 at 357.704 kb on + strand, within BWI76_RS01750 at 357.705 kb on - strand, within BWI76_RS01750 at 357.712 kb on - strand, within BWI76_RS01750 at 357.719 kb on + strand, within BWI76_RS01750 at 357.724 kb on + strand, within BWI76_RS01750 at 357.724 kb on + strand, within BWI76_RS01750 at 357.724 kb on + strand, within BWI76_RS01750 at 357.730 kb on - strand, within BWI76_RS01750 at 357.732 kb on - strand, within BWI76_RS01750 at 357.739 kb on - strand, within BWI76_RS01750 at 357.788 kb on - strand, within BWI76_RS01750 at 357.796 kb on + strand, within BWI76_RS01750 at 357.797 kb on + strand, within BWI76_RS01750 at 357.935 kb on + strand, within BWI76_RS01750 at 358.035 kb on + strand, within BWI76_RS01750 at 358.134 kb on + strand, within BWI76_RS01750 at 358.164 kb on - strand, within BWI76_RS01750 at 358.184 kb on - strand, within BWI76_RS01750 at 358.216 kb on + strand, within BWI76_RS01750 at 358.255 kb on - strand, within BWI76_RS01750 at 358.281 kb on - strand, within BWI76_RS01750 at 358.347 kb on + strand at 358.347 kb on + strand at 358.347 kb on + strand at 358.355 kb on - strand at 358.355 kb on - strand at 358.365 kb on - strand at 358.396 kb on - strand at 358.438 kb on - strand at 358.547 kb on + strand at 358.568 kb on - strand at 358.578 kb on + strand at 358.586 kb on - strand at 358.647 kb on - strand at 358.654 kb on + strand at 358.688 kb on + strand at 358.723 kb on + strand at 358.737 kb on - strand at 358.825 kb on + strand at 358.825 kb on + strand at 358.833 kb on - strand at 358.833 kb on - strand at 358.884 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.967 kb on - strand, within BWI76_RS01755 at 358.978 kb on + strand, within BWI76_RS01755 at 359.091 kb on + strand, within BWI76_RS01755 at 359.191 kb on + strand, within BWI76_RS01755 at 359.252 kb on - strand, within BWI76_RS01755
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 356,500 - BWI76_RS01740 0.79 +0.2 356,509 + BWI76_RS01740 0.82 +0.3 356,514 + BWI76_RS01740 0.83 +1.2 356,517 - BWI76_RS01740 0.84 +0.8 356,536 - BWI76_RS01740 0.88 +0.9 356,545 + -1.4 356,545 + +0.8 356,575 - +0.7 356,767 + BWI76_RS01745 0.22 -0.3 356,791 - BWI76_RS01745 0.25 -1.8 356,798 - BWI76_RS01745 0.25 -0.8 356,857 - BWI76_RS01745 0.33 +1.4 356,883 + BWI76_RS01745 0.36 +0.1 356,883 + BWI76_RS01745 0.36 +1.8 356,883 + BWI76_RS01745 0.36 -0.5 356,891 - BWI76_RS01745 0.37 -3.3 356,901 - BWI76_RS01745 0.38 -0.3 356,913 + BWI76_RS01745 0.40 +0.6 356,923 + BWI76_RS01745 0.41 +0.4 356,931 - BWI76_RS01745 0.42 -0.5 356,948 - BWI76_RS01745 0.44 -1.4 356,973 + BWI76_RS01745 0.47 -0.4 356,986 + BWI76_RS01745 0.49 -0.8 356,986 + BWI76_RS01745 0.49 +1.8 356,986 + BWI76_RS01745 0.49 -2.9 356,986 - BWI76_RS01745 0.49 -1.9 356,988 + BWI76_RS01745 0.49 +0.5 356,988 + BWI76_RS01745 0.49 -2.6 356,988 + BWI76_RS01745 0.49 +0.1 356,988 + BWI76_RS01745 0.49 +0.2 356,994 - BWI76_RS01745 0.50 -0.8 356,996 - +1.2 356,996 - BWI76_RS01745 0.50 +0.6 356,996 - BWI76_RS01745 0.50 +0.3 356,996 - BWI76_RS01745 0.50 +0.1 356,996 - BWI76_RS01745 0.50 +0.1 356,996 - BWI76_RS01745 0.50 +0.2 357,019 - BWI76_RS01745 0.53 -2.2 357,032 + BWI76_RS01745 0.55 +1.2 357,072 + BWI76_RS01745 0.60 +1.0 357,073 + BWI76_RS01745 0.60 -0.4 357,080 - BWI76_RS01745 0.61 +0.6 357,104 - BWI76_RS01745 0.64 +1.8 357,104 - BWI76_RS01745 0.64 +1.7 357,127 + BWI76_RS01745 0.66 -0.4 357,127 - BWI76_RS01745 0.66 -0.3 357,132 + BWI76_RS01745 0.67 +0.4 357,135 - BWI76_RS01745 0.67 +1.2 357,137 + BWI76_RS01745 0.68 -1.8 357,139 - BWI76_RS01745 0.68 -0.4 357,140 - BWI76_RS01745 0.68 +0.2 357,145 - BWI76_RS01745 0.69 +1.2 357,148 - BWI76_RS01745 0.69 -3.0 357,153 - +0.6 357,160 - BWI76_RS01745 0.71 -1.6 357,169 - BWI76_RS01745 0.72 -1.8 357,171 - +0.2 357,182 - BWI76_RS01745 0.73 -1.3 357,199 - BWI76_RS01745 0.75 +0.2 357,208 + BWI76_RS01745 0.76 -3.9 357,209 - +0.7 357,222 - BWI76_RS01745 0.78 -1.6 357,235 - BWI76_RS01745 0.80 -0.2 357,245 + BWI76_RS01745 0.81 -0.5 357,245 + BWI76_RS01745 0.81 +0.2 357,258 - BWI76_RS01745 0.83 -2.2 357,292 - BWI76_RS01745 0.87 -0.4 357,313 + BWI76_RS01745 0.90 -0.4 357,313 - BWI76_RS01745 0.90 +0.6 357,321 - -0.6 357,416 + +0.5 357,424 - -0.1 357,434 + -1.4 357,434 + -0.4 357,434 + -2.0 357,434 + -0.6 357,434 + -3.3 357,434 + -2.2 357,434 + -3.3 357,437 - +0.9 357,439 - -2.3 357,442 - -0.2 357,442 - +0.1 357,442 - -2.7 357,442 - +0.6 357,442 - +2.3 357,442 - +1.1 357,474 + -0.3 357,481 + -2.9 357,481 + +2.3 357,481 + -1.6 357,481 + +1.2 357,481 + -0.8 357,481 + -1.3 357,481 + +0.2 357,481 + +0.3 357,481 + -1.0 357,489 - -0.4 357,489 - +1.3 357,489 - +0.3 357,489 - -0.5 357,489 - +0.0 357,489 - +1.0 357,489 - +1.7 357,489 - -1.2 357,489 - -1.6 357,489 - +0.4 357,513 - -0.8 357,513 - +1.2 357,518 - +0.5 357,525 + -0.1 357,527 + +0.5 357,527 + +0.1 357,527 + +0.5 357,533 - -1.2 357,533 - +1.2 357,533 - -0.6 357,534 + -0.4 357,534 + -1.7 357,535 - -0.1 357,535 - -2.5 357,535 - -0.1 357,542 - -1.8 357,551 + +1.5 357,551 + -0.4 357,576 - BWI76_RS01750 0.11 +2.2 357,577 + BWI76_RS01750 0.12 -1.0 357,577 + BWI76_RS01750 0.12 +1.1 357,577 + BWI76_RS01750 0.12 +0.2 357,577 + BWI76_RS01750 0.12 +1.5 357,577 + BWI76_RS01750 0.12 +1.4 357,579 + BWI76_RS01750 0.12 -0.3 357,585 - BWI76_RS01750 0.12 +0.8 357,585 - BWI76_RS01750 0.12 +0.9 357,587 - BWI76_RS01750 0.13 +0.2 357,594 + BWI76_RS01750 0.13 -0.8 357,599 + BWI76_RS01750 0.14 +1.4 357,602 - BWI76_RS01750 0.14 -1.2 357,602 - BWI76_RS01750 0.14 -0.8 357,608 - BWI76_RS01750 0.15 +1.7 357,610 + BWI76_RS01750 0.15 -1.3 357,618 - BWI76_RS01750 0.16 -0.7 357,618 - BWI76_RS01750 0.16 +2.8 357,618 - BWI76_RS01750 0.16 +0.8 357,618 - BWI76_RS01750 0.16 +0.2 357,644 + BWI76_RS01750 0.19 +0.2 357,645 - BWI76_RS01750 0.19 +0.6 357,648 + BWI76_RS01750 0.19 +0.1 357,656 - BWI76_RS01750 0.20 +0.8 357,656 - BWI76_RS01750 0.20 +0.7 357,656 - BWI76_RS01750 0.20 -0.8 357,663 + BWI76_RS01750 0.21 -1.5 357,704 + BWI76_RS01750 0.25 +1.4 357,705 - BWI76_RS01750 0.25 -0.5 357,712 - BWI76_RS01750 0.26 +0.5 357,719 + BWI76_RS01750 0.27 +0.2 357,724 + BWI76_RS01750 0.27 +0.3 357,724 + BWI76_RS01750 0.27 +1.2 357,724 + BWI76_RS01750 0.27 +0.9 357,730 - BWI76_RS01750 0.28 -0.4 357,732 - BWI76_RS01750 0.28 -0.6 357,739 - BWI76_RS01750 0.29 +1.1 357,788 - BWI76_RS01750 0.34 -2.9 357,796 + BWI76_RS01750 0.35 +0.2 357,797 + BWI76_RS01750 0.35 +0.3 357,935 + BWI76_RS01750 0.49 +1.0 358,035 + BWI76_RS01750 0.60 +1.0 358,134 + BWI76_RS01750 0.70 -0.9 358,164 - BWI76_RS01750 0.74 +1.1 358,184 - BWI76_RS01750 0.76 -3.1 358,216 + BWI76_RS01750 0.79 +0.9 358,255 - BWI76_RS01750 0.83 +1.4 358,281 - BWI76_RS01750 0.86 -0.1 358,347 + -0.1 358,347 + -0.8 358,347 + +1.6 358,355 - +1.2 358,355 - -1.2 358,365 - -2.9 358,396 - +0.1 358,438 - +1.2 358,547 + -1.2 358,568 - -0.2 358,578 + +0.2 358,586 - +3.8 358,647 - +1.3 358,654 + -1.0 358,688 + +1.2 358,723 + +1.3 358,737 - +1.2 358,825 + -0.6 358,825 + -1.8 358,833 - +1.2 358,833 - -0.9 358,884 + BWI76_RS01755 0.14 +0.2 358,897 + BWI76_RS01755 0.15 +1.6 358,897 + BWI76_RS01755 0.15 -1.1 358,897 + BWI76_RS01755 0.15 +0.2 358,897 + BWI76_RS01755 0.15 -0.6 358,967 - BWI76_RS01755 0.23 +1.3 358,978 + BWI76_RS01755 0.25 -2.9 359,091 + BWI76_RS01755 0.38 -1.1 359,191 + BWI76_RS01755 0.49 -0.8 359,252 - BWI76_RS01755 0.56 +1.4
Or see this region's nucleotide sequence