Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01275 and BWI76_RS01280 are separated by 232 nucleotides BWI76_RS01280 and BWI76_RS01285 are separated by 165 nucleotides BWI76_RS01285 and BWI76_RS01290 are separated by 3 nucleotides
BWI76_RS01275: BWI76_RS01275 - LysR family transcriptional regulator, at 260,795 to 261,748
_RS01275
BWI76_RS01280: BWI76_RS01280 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 261,981 to 264,242
_RS01280
BWI76_RS01285: BWI76_RS01285 - amino acid ABC transporter substrate-binding protein, at 264,408 to 265,097
_RS01285
BWI76_RS01290: BWI76_RS01290 - putative dienelactone hydrolase, at 265,101 to 265,913
_RS01290
Position (kb) 261
262
263
264
265 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 260.996 kb on + strand, within BWI76_RS01275 at 261.005 kb on - strand, within BWI76_RS01275 at 261.109 kb on - strand, within BWI76_RS01275 at 261.114 kb on - strand, within BWI76_RS01275 at 261.435 kb on - strand, within BWI76_RS01275 at 261.625 kb on + strand, within BWI76_RS01275 at 261.633 kb on - strand, within BWI76_RS01275 at 261.663 kb on + strand at 261.699 kb on + strand at 261.904 kb on - strand at 262.023 kb on - strand at 262.089 kb on - strand at 262.183 kb on + strand at 262.201 kb on + strand at 262.226 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.517 kb on - strand, within BWI76_RS01280 at 262.692 kb on - strand, within BWI76_RS01280 at 262.692 kb on - strand, within BWI76_RS01280 at 262.901 kb on + strand, within BWI76_RS01280 at 262.909 kb on - strand, within BWI76_RS01280 at 263.014 kb on + strand, within BWI76_RS01280 at 263.072 kb on - strand, within BWI76_RS01280 at 263.247 kb on - strand, within BWI76_RS01280 at 263.290 kb on + strand, within BWI76_RS01280 at 263.298 kb on - strand, within BWI76_RS01280 at 263.345 kb on + strand, within BWI76_RS01280 at 263.406 kb on + strand, within BWI76_RS01280 at 263.459 kb on - strand, within BWI76_RS01280 at 263.513 kb on - strand, within BWI76_RS01280 at 263.518 kb on - strand, within BWI76_RS01280 at 263.525 kb on - strand, within BWI76_RS01280 at 263.535 kb on - strand, within BWI76_RS01280 at 263.901 kb on + strand, within BWI76_RS01280 at 263.906 kb on + strand, within BWI76_RS01280 at 264.050 kb on + strand at 264.087 kb on - strand at 264.104 kb on + strand at 264.142 kb on + strand at 264.217 kb on - strand at 264.217 kb on - strand at 264.217 kb on - strand at 264.319 kb on - strand at 264.367 kb on - strand at 264.448 kb on + strand at 264.505 kb on + strand, within BWI76_RS01285 at 264.568 kb on - strand, within BWI76_RS01285 at 264.662 kb on - strand, within BWI76_RS01285 at 264.761 kb on - strand, within BWI76_RS01285 at 264.958 kb on + strand, within BWI76_RS01285 at 264.992 kb on + strand, within BWI76_RS01285 at 265.007 kb on - strand, within BWI76_RS01285 at 265.060 kb on - strand at 265.099 kb on - strand at 265.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 260,996 + BWI76_RS01275 0.21 -0.4 261,005 - BWI76_RS01275 0.22 -2.5 261,109 - BWI76_RS01275 0.33 -3.2 261,114 - BWI76_RS01275 0.33 +0.2 261,435 - BWI76_RS01275 0.67 -2.8 261,625 + BWI76_RS01275 0.87 -4.2 261,633 - BWI76_RS01275 0.88 -1.7 261,663 + -2.7 261,699 + -3.9 261,904 - -1.6 262,023 - +0.3 262,089 - -1.3 262,183 + -3.0 262,201 + -2.7 262,226 - BWI76_RS01280 0.11 -1.5 262,504 - BWI76_RS01280 0.23 -3.3 262,504 - BWI76_RS01280 0.23 +0.1 262,504 - BWI76_RS01280 0.23 -1.2 262,504 - BWI76_RS01280 0.23 -2.0 262,504 - BWI76_RS01280 0.23 -1.1 262,504 - BWI76_RS01280 0.23 -3.1 262,517 - BWI76_RS01280 0.24 -1.6 262,692 - BWI76_RS01280 0.31 -3.4 262,692 - BWI76_RS01280 0.31 -0.4 262,901 + BWI76_RS01280 0.41 +0.8 262,909 - BWI76_RS01280 0.41 -0.6 263,014 + BWI76_RS01280 0.46 -1.0 263,072 - BWI76_RS01280 0.48 -1.6 263,247 - BWI76_RS01280 0.56 -0.4 263,290 + BWI76_RS01280 0.58 -3.4 263,298 - BWI76_RS01280 0.58 -1.1 263,345 + BWI76_RS01280 0.60 -2.7 263,406 + BWI76_RS01280 0.63 -0.9 263,459 - BWI76_RS01280 0.65 +0.2 263,513 - BWI76_RS01280 0.68 -3.0 263,518 - BWI76_RS01280 0.68 -0.8 263,525 - BWI76_RS01280 0.68 -0.4 263,535 - BWI76_RS01280 0.69 -0.7 263,901 + BWI76_RS01280 0.85 -1.5 263,906 + BWI76_RS01280 0.85 -1.4 264,050 + -0.6 264,087 - -3.4 264,104 + -2.6 264,142 + -2.2 264,217 - -1.9 264,217 - -1.1 264,217 - +0.2 264,319 - -1.0 264,367 - +1.2 264,448 + -0.3 264,505 + BWI76_RS01285 0.14 +2.5 264,568 - BWI76_RS01285 0.23 +1.1 264,662 - BWI76_RS01285 0.37 -0.4 264,761 - BWI76_RS01285 0.51 +1.8 264,958 + BWI76_RS01285 0.80 -0.7 264,992 + BWI76_RS01285 0.85 +1.5 265,007 - BWI76_RS01285 0.87 +0.5 265,060 - +1.2 265,099 - -1.8 265,132 - +1.6
Or see this region's nucleotide sequence