Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35460 and BPHYT_RS35465 are separated by 1 nucleotides BPHYT_RS35465 and BPHYT_RS35470 are separated by 27 nucleotides BPHYT_RS35470 and BPHYT_RS35475 overlap by 4 nucleotides BPHYT_RS35475 and BPHYT_RS35480 overlap by 4 nucleotides
BPHYT_RS35460: BPHYT_RS35460 - acyl-CoA dehydrogenase, at 3,556,125 to 3,557,333
_RS35460
BPHYT_RS35465: BPHYT_RS35465 - enoyl-CoA hydratase, at 3,557,335 to 3,558,186
_RS35465
BPHYT_RS35470: BPHYT_RS35470 - MarR family transcriptional regulator, at 3,558,214 to 3,558,777
_RS35470
BPHYT_RS35475: BPHYT_RS35475 - 3-hydroxyacyl-CoA dehydrogenase, at 3,558,774 to 3,559,547
_RS35475
BPHYT_RS35480: BPHYT_RS35480 - salicylyl-CoA 5-hydroxylase, at 3,559,544 to 3,561,916
_RS35480
Position (kb)
3558
3559 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3557.250 kb on - strand at 3557.737 kb on - strand, within BPHYT_RS35465 at 3557.737 kb on - strand, within BPHYT_RS35465 at 3557.996 kb on + strand, within BPHYT_RS35465 at 3557.997 kb on - strand, within BPHYT_RS35465
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 3,557,250 - -2.8 3,557,737 - BPHYT_RS35465 0.47 +0.7 3,557,737 - BPHYT_RS35465 0.47 +0.6 3,557,996 + BPHYT_RS35465 0.78 +0.7 3,557,997 - BPHYT_RS35465 0.78 +0.2
Or see this region's nucleotide sequence