Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27205

Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27195 and BWI76_RS27200 are separated by 49 nucleotidesBWI76_RS27200 and BWI76_RS27205 are separated by 105 nucleotidesBWI76_RS27205 and BWI76_RS27210 are separated by 80 nucleotides BWI76_RS27195: BWI76_RS27195 - alpha-hydroxy-acid oxidizing enzyme, at 5,645,418 to 5,646,648 _RS27195 BWI76_RS27200: BWI76_RS27200 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 5,646,698 to 5,647,171 _RS27200 BWI76_RS27205: BWI76_RS27205 - serine O-acetyltransferase, at 5,647,277 to 5,648,098 _RS27205 BWI76_RS27210: BWI76_RS27210 - glycerol-3-phosphate dehydrogenase, at 5,648,179 to 5,649,195 _RS27210 Position (kb) 5647 5648 5649Strain fitness (log2 ratio) -3 -2 -1 0 1at 5646.406 kb on + strand, within BWI76_RS27195at 5646.651 kb on - strandat 5646.652 kb on - strandat 5646.866 kb on + strand, within BWI76_RS27200at 5646.924 kb on - strand, within BWI76_RS27200at 5646.966 kb on + strand, within BWI76_RS27200at 5647.005 kb on + strand, within BWI76_RS27200at 5647.322 kb on + strandat 5648.103 kb on - strandat 5648.103 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
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5,646,406 + BWI76_RS27195 0.80 +0.7
5,646,651 - -3.3
5,646,652 - +1.2
5,646,866 + BWI76_RS27200 0.35 +0.4
5,646,924 - BWI76_RS27200 0.48 -0.9
5,646,966 + BWI76_RS27200 0.57 -2.1
5,647,005 + BWI76_RS27200 0.65 -2.1
5,647,322 + -1.4
5,648,103 - +0.6
5,648,103 - +0.2

Or see this region's nucleotide sequence