Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27100 and BWI76_RS27105 overlap by 4 nucleotides BWI76_RS27105 and BWI76_RS27110 overlap by 8 nucleotides BWI76_RS27110 and BWI76_RS27115 overlap by 7 nucleotides BWI76_RS27115 and BWI76_RS27120 are separated by 159 nucleotides
BWI76_RS27100: BWI76_RS27100 - carbohydrate kinase, at 5,623,536 to 5,625,041
_RS27100
BWI76_RS27105: BWI76_RS27105 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 5,625,038 to 5,625,700
_RS27105
BWI76_RS27110: BWI76_RS27110 - xylulose 5-phosphate 3-epimerase, at 5,625,693 to 5,626,553
_RS27110
BWI76_RS27115: BWI76_RS27115 - L-ribulose-5-phosphate 4-epimerase, at 5,626,547 to 5,627,245
_RS27115
BWI76_RS27120: BWI76_RS27120 - AraC family transcriptional regulator, at 5,627,405 to 5,628,208
_RS27120
Position (kb) 5625
5626
5627 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5624.872 kb on + strand, within BWI76_RS27100 at 5624.879 kb on - strand, within BWI76_RS27100 at 5624.944 kb on + strand at 5625.000 kb on + strand at 5625.000 kb on + strand at 5625.013 kb on - strand at 5625.019 kb on + strand at 5625.079 kb on + strand at 5625.099 kb on + strand at 5625.349 kb on - strand, within BWI76_RS27105 at 5625.416 kb on - strand, within BWI76_RS27105 at 5625.458 kb on + strand, within BWI76_RS27105 at 5625.475 kb on + strand, within BWI76_RS27105 at 5625.525 kb on + strand, within BWI76_RS27105 at 5625.549 kb on + strand, within BWI76_RS27105 at 5625.557 kb on - strand, within BWI76_RS27105 at 5625.582 kb on - strand, within BWI76_RS27105 at 5625.590 kb on + strand, within BWI76_RS27105 at 5625.619 kb on + strand, within BWI76_RS27105 at 5625.743 kb on + strand at 5625.756 kb on - strand at 5625.852 kb on - strand, within BWI76_RS27110 at 5625.869 kb on - strand, within BWI76_RS27110 at 5625.947 kb on + strand, within BWI76_RS27110 at 5625.991 kb on - strand, within BWI76_RS27110 at 5626.103 kb on - strand, within BWI76_RS27110 at 5626.118 kb on + strand at 5626.258 kb on - strand at 5626.334 kb on + strand, within BWI76_RS27110 at 5626.351 kb on + strand, within BWI76_RS27110 at 5626.351 kb on + strand, within BWI76_RS27110 at 5626.508 kb on + strand at 5626.614 kb on + strand at 5626.614 kb on + strand at 5626.686 kb on + strand, within BWI76_RS27115 at 5626.686 kb on + strand, within BWI76_RS27115 at 5626.686 kb on + strand, within BWI76_RS27115 at 5626.693 kb on + strand, within BWI76_RS27115 at 5626.775 kb on - strand, within BWI76_RS27115 at 5626.891 kb on - strand, within BWI76_RS27115 at 5626.952 kb on - strand, within BWI76_RS27115 at 5626.980 kb on - strand, within BWI76_RS27115 at 5626.994 kb on + strand, within BWI76_RS27115 at 5627.016 kb on + strand, within BWI76_RS27115 at 5627.024 kb on - strand, within BWI76_RS27115 at 5627.024 kb on - strand, within BWI76_RS27115 at 5627.042 kb on + strand, within BWI76_RS27115 at 5627.064 kb on + strand, within BWI76_RS27115 at 5627.178 kb on + strand at 5627.179 kb on - strand at 5627.281 kb on + strand at 5627.292 kb on - strand at 5627.340 kb on + strand at 5627.348 kb on - strand at 5627.378 kb on - strand at 5627.410 kb on - strand at 5627.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x remove 5,624,872 + BWI76_RS27100 0.89 -0.4 5,624,879 - BWI76_RS27100 0.89 +1.3 5,624,944 + -0.4 5,625,000 + -3.2 5,625,000 + -4.8 5,625,013 - +0.2 5,625,019 + +0.1 5,625,079 + +0.2 5,625,099 + +2.0 5,625,349 - BWI76_RS27105 0.47 +0.1 5,625,416 - BWI76_RS27105 0.57 +0.2 5,625,458 + BWI76_RS27105 0.63 +1.5 5,625,475 + BWI76_RS27105 0.66 +1.1 5,625,525 + BWI76_RS27105 0.73 -0.8 5,625,549 + BWI76_RS27105 0.77 +0.8 5,625,557 - BWI76_RS27105 0.78 +0.4 5,625,582 - BWI76_RS27105 0.82 +1.2 5,625,590 + BWI76_RS27105 0.83 +1.3 5,625,619 + BWI76_RS27105 0.88 -1.6 5,625,743 + +0.4 5,625,756 - -1.0 5,625,852 - BWI76_RS27110 0.18 -0.2 5,625,869 - BWI76_RS27110 0.20 +1.2 5,625,947 + BWI76_RS27110 0.30 -2.1 5,625,991 - BWI76_RS27110 0.35 -0.1 5,626,103 - BWI76_RS27110 0.48 +0.8 5,626,118 + -0.8 5,626,258 - +2.1 5,626,334 + BWI76_RS27110 0.74 -3.6 5,626,351 + BWI76_RS27110 0.76 -0.1 5,626,351 + BWI76_RS27110 0.76 +0.8 5,626,508 + -2.8 5,626,614 + +1.2 5,626,614 + -0.9 5,626,686 + BWI76_RS27115 0.20 -0.9 5,626,686 + BWI76_RS27115 0.20 -0.2 5,626,686 + BWI76_RS27115 0.20 +0.2 5,626,693 + BWI76_RS27115 0.21 +0.1 5,626,775 - BWI76_RS27115 0.33 +0.3 5,626,891 - BWI76_RS27115 0.49 +0.7 5,626,952 - BWI76_RS27115 0.58 -1.6 5,626,980 - BWI76_RS27115 0.62 -0.6 5,626,994 + BWI76_RS27115 0.64 +0.5 5,627,016 + BWI76_RS27115 0.67 +0.3 5,627,024 - BWI76_RS27115 0.68 -0.1 5,627,024 - BWI76_RS27115 0.68 +0.7 5,627,042 + BWI76_RS27115 0.71 +0.4 5,627,064 + BWI76_RS27115 0.74 +0.5 5,627,178 + +0.9 5,627,179 - -0.1 5,627,281 + -0.7 5,627,292 - +1.2 5,627,340 + +1.2 5,627,348 - -0.2 5,627,378 - -0.3 5,627,410 - -0.7 5,627,449 - -1.6
Or see this region's nucleotide sequence