Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22420 and BWI76_RS22425 are separated by 177 nucleotides BWI76_RS22425 and BWI76_RS22430 overlap by 4 nucleotides BWI76_RS22430 and BWI76_RS22435 overlap by 4 nucleotides
BWI76_RS22420: BWI76_RS22420 - LacI family transcriptional regulator, at 4,675,019 to 4,675,981
_RS22420
BWI76_RS22425: BWI76_RS22425 - metal ABC transporter substrate-binding protein, at 4,676,159 to 4,677,076
_RS22425
BWI76_RS22430: BWI76_RS22430 - manganese/iron transporter ATP-binding protein, at 4,677,073 to 4,677,894
_RS22430
BWI76_RS22435: BWI76_RS22435 - iron ABC transporter permease AfeC, at 4,677,891 to 4,678,739
_RS22435
Position (kb) 4676
4677
4678 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 4675.258 kb on + strand, within BWI76_RS22420 at 4676.162 kb on - strand at 4676.162 kb on - strand at 4676.183 kb on + strand at 4676.191 kb on - strand at 4676.220 kb on - strand at 4676.463 kb on - strand, within BWI76_RS22425 at 4676.521 kb on + strand, within BWI76_RS22425 at 4676.646 kb on - strand, within BWI76_RS22425 at 4676.724 kb on - strand, within BWI76_RS22425 at 4676.764 kb on - strand, within BWI76_RS22425 at 4676.816 kb on - strand, within BWI76_RS22425 at 4677.107 kb on + strand at 4677.363 kb on + strand, within BWI76_RS22430 at 4677.648 kb on + strand, within BWI76_RS22430 at 4677.811 kb on + strand, within BWI76_RS22430 at 4677.819 kb on - strand at 4678.036 kb on + strand, within BWI76_RS22435
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x remove 4,675,258 + BWI76_RS22420 0.25 -3.1 4,676,162 - -0.2 4,676,162 - -2.8 4,676,183 + +0.6 4,676,191 - -0.2 4,676,220 - -2.6 4,676,463 - BWI76_RS22425 0.33 -0.4 4,676,521 + BWI76_RS22425 0.39 -0.4 4,676,646 - BWI76_RS22425 0.53 +0.5 4,676,724 - BWI76_RS22425 0.62 +1.9 4,676,764 - BWI76_RS22425 0.66 +0.4 4,676,816 - BWI76_RS22425 0.72 +1.1 4,677,107 + -2.4 4,677,363 + BWI76_RS22430 0.35 -1.3 4,677,648 + BWI76_RS22430 0.70 +0.6 4,677,811 + BWI76_RS22430 0.90 +2.5 4,677,819 - -4.8 4,678,036 + BWI76_RS22435 0.17 +0.7
Or see this region's nucleotide sequence