Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20240

Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20235 and BWI76_RS20240 are separated by 76 nucleotidesBWI76_RS20240 and BWI76_RS20245 are separated by 46 nucleotidesBWI76_RS20245 and BWI76_RS20250 overlap by 8 nucleotides BWI76_RS20235: BWI76_RS20235 - acetate kinase, at 4,240,377 to 4,241,579 _RS20235 BWI76_RS20240: BWI76_RS20240 - phosphate acetyltransferase, at 4,241,656 to 4,243,800 _RS20240 BWI76_RS20245: BWI76_RS20245 - transketolase subunit B, at 4,243,847 to 4,244,800 _RS20245 BWI76_RS20250: BWI76_RS20250 - hypothetical protein, at 4,244,793 to 4,245,623 _RS20250 Position (kb) 4241 4242 4243 4244Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4240.747 kb on + strand, within BWI76_RS20235at 4241.607 kb on + strandat 4241.964 kb on - strand, within BWI76_RS20240at 4242.998 kb on + strand, within BWI76_RS20240at 4243.710 kb on + strandat 4243.750 kb on + strandat 4243.928 kb on - strandat 4243.942 kb on + strandat 4243.976 kb on + strand, within BWI76_RS20245at 4244.137 kb on - strand, within BWI76_RS20245at 4244.455 kb on + strand, within BWI76_RS20245at 4244.507 kb on - strand, within BWI76_RS20245at 4244.668 kb on + strand, within BWI76_RS20245at 4244.742 kb on + strandat 4244.791 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
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4,240,747 + BWI76_RS20235 0.31 +0.8
4,241,607 + -4.1
4,241,964 - BWI76_RS20240 0.14 +1.6
4,242,998 + BWI76_RS20240 0.63 +2.0
4,243,710 + -0.4
4,243,750 + -1.4
4,243,928 - -0.5
4,243,942 + +1.5
4,243,976 + BWI76_RS20245 0.14 -1.8
4,244,137 - BWI76_RS20245 0.30 -0.1
4,244,455 + BWI76_RS20245 0.64 +0.9
4,244,507 - BWI76_RS20245 0.69 -2.7
4,244,668 + BWI76_RS20245 0.86 +0.2
4,244,742 + -2.4
4,244,791 + +1.1

Or see this region's nucleotide sequence