Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20050

Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20045 and BWI76_RS20050 are separated by 105 nucleotidesBWI76_RS20050 and BWI76_RS20055 overlap by 24 nucleotides BWI76_RS20045: BWI76_RS20045 - hypothetical protein, at 4,198,990 to 4,200,510 _RS20045 BWI76_RS20050: BWI76_RS20050 - hypothetical protein, at 4,200,616 to 4,201,239 _RS20050 BWI76_RS20055: BWI76_RS20055 - 2-succinylbenzoate-CoA ligase, at 4,201,216 to 4,202,607 _RS20055 Position (kb) 4200 4201 4202Strain fitness (log2 ratio) -3 -2 -1 0 1at 4199.825 kb on + strand, within BWI76_RS20045at 4200.145 kb on - strand, within BWI76_RS20045at 4200.169 kb on - strand, within BWI76_RS20045at 4200.844 kb on + strand, within BWI76_RS20050at 4201.569 kb on - strand, within BWI76_RS20055at 4201.754 kb on - strand, within BWI76_RS20055at 4202.020 kb on - strand, within BWI76_RS20055

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
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4,199,825 + BWI76_RS20045 0.55 +1.1
4,200,145 - BWI76_RS20045 0.76 -2.8
4,200,169 - BWI76_RS20045 0.78 -0.3
4,200,844 + BWI76_RS20050 0.37 -2.1
4,201,569 - BWI76_RS20055 0.25 -1.3
4,201,754 - BWI76_RS20055 0.39 -0.2
4,202,020 - BWI76_RS20055 0.58 -0.8

Or see this region's nucleotide sequence