Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08745

Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08740 and BWI76_RS08745 are separated by 100 nucleotidesBWI76_RS08745 and BWI76_RS08750 are separated by 104 nucleotidesBWI76_RS08750 and BWI76_RS08755 are separated by 69 nucleotides BWI76_RS08740: BWI76_RS08740 - Histidine ammonia-lyase (EC 4.3.1.3) (from data), at 1,841,384 to 1,842,907 _RS08740 BWI76_RS08745: BWI76_RS08745 - proline-specific permease, at 1,843,008 to 1,844,387 _RS08745 BWI76_RS08750: BWI76_RS08750 - kinase inhibitor, at 1,844,492 to 1,844,968 _RS08750 BWI76_RS08755: BWI76_RS08755 - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 1,845,038 to 1,846,327 _RS08755 Position (kb) 1843 1844 1845Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1842.064 kb on - strand, within BWI76_RS08740at 1842.136 kb on + strandat 1842.263 kb on + strand, within BWI76_RS08740at 1842.272 kb on - strand, within BWI76_RS08740at 1842.482 kb on - strand, within BWI76_RS08740at 1842.597 kb on + strand, within BWI76_RS08740at 1843.359 kb on - strand, within BWI76_RS08745at 1843.361 kb on + strand, within BWI76_RS08745at 1843.748 kb on + strand, within BWI76_RS08745at 1844.312 kb on + strandat 1844.312 kb on + strandat 1844.867 kb on - strand, within BWI76_RS08750at 1845.273 kb on - strand, within BWI76_RS08755

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
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1,842,064 - BWI76_RS08740 0.45 +0.7
1,842,136 + +1.2
1,842,263 + BWI76_RS08740 0.58 +1.2
1,842,272 - BWI76_RS08740 0.58 -0.4
1,842,482 - BWI76_RS08740 0.72 +1.1
1,842,597 + BWI76_RS08740 0.80 +1.7
1,843,359 - BWI76_RS08745 0.25 +2.0
1,843,361 + BWI76_RS08745 0.26 +0.3
1,843,748 + BWI76_RS08745 0.54 +0.5
1,844,312 + +0.3
1,844,312 + +1.5
1,844,867 - BWI76_RS08750 0.79 -4.4
1,845,273 - BWI76_RS08755 0.18 +2.8

Or see this region's nucleotide sequence