Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04515

Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04505 and BWI76_RS04510 are separated by 40 nucleotidesBWI76_RS04510 and BWI76_RS04515 are separated by 4 nucleotidesBWI76_RS04515 and BWI76_RS04520 are separated by 2 nucleotidesBWI76_RS04520 and BWI76_RS04525 overlap by 4 nucleotides BWI76_RS04505: BWI76_RS04505 - dihydrofolate reductase, at 932,871 to 933,350 _RS04505 BWI76_RS04510: BWI76_RS04510 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 933,391 to 934,239 _RS04510 BWI76_RS04515: BWI76_RS04515 - Co2+/Mg2+ efflux protein ApaG, at 934,244 to 934,621 _RS04515 BWI76_RS04520: BWI76_RS04520 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase, at 934,624 to 935,445 _RS04520 BWI76_RS04525: BWI76_RS04525 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 935,442 to 936,431 _RS04525 Position (kb) 934 935Strain fitness (log2 ratio) -3 -2 -1 0 1at 933.478 kb on + strand, within BWI76_RS04510at 933.624 kb on + strand, within BWI76_RS04510at 934.061 kb on - strand, within BWI76_RS04510at 934.344 kb on - strand, within BWI76_RS04515at 934.605 kb on - strandat 935.290 kb on + strand, within BWI76_RS04520at 935.422 kb on + strandat 935.511 kb on + strandat 935.533 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
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933,478 + BWI76_RS04510 0.10 +1.2
933,624 + BWI76_RS04510 0.27 -0.4
934,061 - BWI76_RS04510 0.79 +1.2
934,344 - BWI76_RS04515 0.26 -3.6
934,605 - -2.4
935,290 + BWI76_RS04520 0.81 +1.2
935,422 + +1.2
935,511 + -0.4
935,533 - -1.3

Or see this region's nucleotide sequence