Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04450

Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04440 and BWI76_RS04445 overlap by 4 nucleotidesBWI76_RS04445 and BWI76_RS04450 are separated by 10 nucleotidesBWI76_RS04450 and BWI76_RS04455 are separated by 19 nucleotides BWI76_RS04440: BWI76_RS04440 - citrate lyase ACP, at 917,395 to 917,688 _RS04440 BWI76_RS04445: BWI76_RS04445 - citrate (pro-3S)-lyase subunit beta, at 917,685 to 918,554 _RS04445 BWI76_RS04450: BWI76_RS04450 - citrate lyase subunit alpha, at 918,565 to 920,091 _RS04450 BWI76_RS04455: BWI76_RS04455 - triphosphoribosyl-dephospho-CoA synthase CitG, at 920,111 to 921,019 _RS04455 Position (kb) 918 919 920 921Strain fitness (log2 ratio) -2 -1 0 1at 917.615 kb on - strand, within BWI76_RS04440at 917.653 kb on + strand, within BWI76_RS04440at 917.759 kb on - strandat 917.935 kb on + strand, within BWI76_RS04445at 918.031 kb on + strand, within BWI76_RS04445at 918.031 kb on + strand, within BWI76_RS04445at 918.046 kb on + strand, within BWI76_RS04445at 918.379 kb on - strand, within BWI76_RS04445at 918.454 kb on + strand, within BWI76_RS04445at 918.527 kb on - strandat 918.741 kb on + strand, within BWI76_RS04450at 918.749 kb on - strand, within BWI76_RS04450at 918.914 kb on + strand, within BWI76_RS04450at 918.914 kb on + strand, within BWI76_RS04450at 918.938 kb on + strand, within BWI76_RS04450at 919.454 kb on + strand, within BWI76_RS04450at 919.509 kb on + strand, within BWI76_RS04450at 919.529 kb on - strand, within BWI76_RS04450at 919.639 kb on + strand, within BWI76_RS04450at 920.000 kb on - strandat 920.081 kb on - strandat 920.159 kb on - strandat 920.251 kb on + strand, within BWI76_RS04455at 920.281 kb on - strand, within BWI76_RS04455at 920.283 kb on + strand, within BWI76_RS04455at 920.350 kb on + strand, within BWI76_RS04455at 920.372 kb on - strand, within BWI76_RS04455at 920.479 kb on - strand, within BWI76_RS04455at 920.497 kb on - strand, within BWI76_RS04455at 920.689 kb on + strand, within BWI76_RS04455at 920.728 kb on + strand, within BWI76_RS04455at 920.873 kb on + strand, within BWI76_RS04455at 920.952 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
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917,615 - BWI76_RS04440 0.75 -0.8
917,653 + BWI76_RS04440 0.88 -0.9
917,759 - -0.6
917,935 + BWI76_RS04445 0.29 +1.5
918,031 + BWI76_RS04445 0.40 +1.1
918,031 + BWI76_RS04445 0.40 -0.5
918,046 + BWI76_RS04445 0.41 -1.8
918,379 - BWI76_RS04445 0.80 -0.5
918,454 + BWI76_RS04445 0.88 -0.7
918,527 - +0.6
918,741 + BWI76_RS04450 0.12 -0.6
918,749 - BWI76_RS04450 0.12 -1.7
918,914 + BWI76_RS04450 0.23 +1.2
918,914 + BWI76_RS04450 0.23 +0.4
918,938 + BWI76_RS04450 0.24 -0.8
919,454 + BWI76_RS04450 0.58 +0.4
919,509 + BWI76_RS04450 0.62 -1.8
919,529 - BWI76_RS04450 0.63 -1.4
919,639 + BWI76_RS04450 0.70 +0.2
920,000 - -0.3
920,081 - +0.6
920,159 - +0.6
920,251 + BWI76_RS04455 0.15 -0.4
920,281 - BWI76_RS04455 0.19 -2.4
920,283 + BWI76_RS04455 0.19 -2.5
920,350 + BWI76_RS04455 0.26 -0.4
920,372 - BWI76_RS04455 0.29 -0.6
920,479 - BWI76_RS04455 0.40 -0.3
920,497 - BWI76_RS04455 0.42 +1.3
920,689 + BWI76_RS04455 0.64 -0.8
920,728 + BWI76_RS04455 0.68 +1.1
920,873 + BWI76_RS04455 0.84 -1.8
920,952 + -1.6

Or see this region's nucleotide sequence