Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04035 and BWI76_RS04040 are separated by 13 nucleotides BWI76_RS04040 and BWI76_RS04045 are separated by 341 nucleotides BWI76_RS04045 and BWI76_RS04050 are separated by 47 nucleotides BWI76_RS04050 and BWI76_RS04055 are separated by 108 nucleotides
BWI76_RS04035: BWI76_RS04035 - LuxR family transcriptional regulator, at 834,420 to 835,100
_RS04035
BWI76_RS04040: BWI76_RS04040 - cys-tRNA(pro)/cys-tRNA(cys) deacylase, at 835,114 to 835,572
_RS04040
BWI76_RS04045: BWI76_RS04045 - PTS system lactose/cellobiose-specific transporter subunit IIC, at 835,914 to 837,257
_RS04045
BWI76_RS04050: BWI76_RS04050 - hydroxamate siderophore iron reductase FhuF, at 837,305 to 838,093
_RS04050
BWI76_RS04055: BWI76_RS04055 - GGDEF domain-containing protein, at 838,202 to 839,248
_RS04055
Position (kb) 835
836
837
838 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 834.916 kb on - strand, within BWI76_RS04035 at 834.920 kb on + strand, within BWI76_RS04035 at 834.980 kb on + strand, within BWI76_RS04035 at 834.980 kb on + strand, within BWI76_RS04035 at 834.988 kb on - strand, within BWI76_RS04035 at 834.988 kb on - strand, within BWI76_RS04035 at 834.995 kb on - strand, within BWI76_RS04035 at 834.995 kb on - strand, within BWI76_RS04035 at 834.997 kb on + strand, within BWI76_RS04035 at 834.997 kb on + strand, within BWI76_RS04035 at 834.997 kb on + strand at 835.004 kb on + strand, within BWI76_RS04035 at 835.004 kb on + strand, within BWI76_RS04035 at 835.005 kb on - strand, within BWI76_RS04035 at 835.005 kb on - strand, within BWI76_RS04035 at 835.005 kb on - strand, within BWI76_RS04035 at 835.009 kb on + strand, within BWI76_RS04035 at 835.009 kb on + strand, within BWI76_RS04035 at 835.014 kb on + strand, within BWI76_RS04035 at 835.014 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.020 kb on + strand, within BWI76_RS04035 at 835.028 kb on - strand, within BWI76_RS04035 at 835.028 kb on - strand, within BWI76_RS04035 at 835.028 kb on - strand, within BWI76_RS04035 at 835.028 kb on - strand, within BWI76_RS04035 at 835.028 kb on - strand, within BWI76_RS04035 at 835.028 kb on - strand, within BWI76_RS04035 at 835.028 kb on - strand, within BWI76_RS04035 at 835.063 kb on + strand at 835.070 kb on - strand at 835.072 kb on + strand at 835.078 kb on + strand at 835.080 kb on + strand at 835.080 kb on + strand at 835.080 kb on + strand at 835.087 kb on + strand at 835.087 kb on + strand at 835.087 kb on + strand at 835.088 kb on - strand at 835.090 kb on + strand at 835.095 kb on + strand at 835.095 kb on - strand at 835.095 kb on - strand at 835.095 kb on - strand at 835.098 kb on - strand at 835.100 kb on + strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.101 kb on + strand at 835.103 kb on + strand at 835.129 kb on + strand at 835.133 kb on - strand at 835.139 kb on + strand at 835.142 kb on + strand at 835.150 kb on - strand at 835.166 kb on + strand, within BWI76_RS04040 at 835.174 kb on - strand, within BWI76_RS04040 at 835.174 kb on - strand, within BWI76_RS04040 at 835.207 kb on - strand, within BWI76_RS04040 at 835.207 kb on - strand, within BWI76_RS04040 at 835.212 kb on + strand, within BWI76_RS04040 at 835.219 kb on - strand, within BWI76_RS04040 at 835.220 kb on - strand, within BWI76_RS04040 at 835.224 kb on + strand, within BWI76_RS04040 at 835.232 kb on - strand, within BWI76_RS04040 at 835.246 kb on - strand, within BWI76_RS04040 at 835.274 kb on - strand, within BWI76_RS04040 at 835.282 kb on - strand, within BWI76_RS04040 at 835.286 kb on + strand, within BWI76_RS04040 at 835.453 kb on + strand, within BWI76_RS04040 at 835.482 kb on + strand, within BWI76_RS04040 at 835.482 kb on + strand, within BWI76_RS04040 at 835.490 kb on - strand, within BWI76_RS04040 at 835.493 kb on + strand, within BWI76_RS04040 at 835.493 kb on + strand, within BWI76_RS04040 at 835.493 kb on + strand, within BWI76_RS04040 at 835.501 kb on - strand, within BWI76_RS04040 at 835.506 kb on - strand, within BWI76_RS04040 at 835.529 kb on + strand at 835.569 kb on - strand at 835.569 kb on - strand at 835.584 kb on + strand at 835.592 kb on - strand at 835.784 kb on + strand at 835.897 kb on - strand at 835.897 kb on - strand at 835.899 kb on + strand at 835.907 kb on - strand at 835.996 kb on - strand at 836.154 kb on - strand, within BWI76_RS04045 at 836.348 kb on - strand, within BWI76_RS04045 at 836.524 kb on + strand, within BWI76_RS04045 at 836.531 kb on - strand, within BWI76_RS04045 at 836.660 kb on - strand, within BWI76_RS04045 at 836.675 kb on - strand, within BWI76_RS04045 at 836.842 kb on + strand, within BWI76_RS04045 at 837.008 kb on + strand, within BWI76_RS04045 at 837.011 kb on - strand, within BWI76_RS04045 at 837.016 kb on - strand, within BWI76_RS04045 at 837.078 kb on - strand, within BWI76_RS04045 at 837.143 kb on - strand at 837.337 kb on - strand at 837.366 kb on - strand at 837.434 kb on - strand, within BWI76_RS04050 at 837.572 kb on + strand, within BWI76_RS04050 at 837.777 kb on - strand at 837.839 kb on + strand, within BWI76_RS04050 at 837.906 kb on + strand, within BWI76_RS04050 at 837.946 kb on - strand, within BWI76_RS04050 at 837.956 kb on - strand, within BWI76_RS04050 at 837.970 kb on - strand, within BWI76_RS04050 at 838.030 kb on + strand at 838.032 kb on + strand at 838.032 kb on + strand at 838.032 kb on + strand at 838.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x remove 834,916 - BWI76_RS04035 0.73 +1.8 834,920 + BWI76_RS04035 0.73 -2.3 834,980 + BWI76_RS04035 0.82 -0.1 834,980 + BWI76_RS04035 0.82 -2.1 834,988 - BWI76_RS04035 0.83 -1.1 834,988 - BWI76_RS04035 0.83 -3.0 834,995 - BWI76_RS04035 0.84 -2.6 834,995 - BWI76_RS04035 0.84 +0.9 834,997 + BWI76_RS04035 0.85 +0.8 834,997 + BWI76_RS04035 0.85 -1.6 834,997 + +0.2 835,004 + BWI76_RS04035 0.86 -3.4 835,004 + BWI76_RS04035 0.86 +0.7 835,005 - BWI76_RS04035 0.86 -0.3 835,005 - BWI76_RS04035 0.86 +1.2 835,005 - BWI76_RS04035 0.86 -1.6 835,009 + BWI76_RS04035 0.86 -0.3 835,009 + BWI76_RS04035 0.86 -1.2 835,014 + BWI76_RS04035 0.87 +0.2 835,014 + BWI76_RS04035 0.87 +1.1 835,020 + BWI76_RS04035 0.88 -1.1 835,020 + BWI76_RS04035 0.88 +0.2 835,020 + BWI76_RS04035 0.88 -0.5 835,020 + BWI76_RS04035 0.88 -0.7 835,020 + BWI76_RS04035 0.88 +0.1 835,020 + BWI76_RS04035 0.88 -3.5 835,020 + BWI76_RS04035 0.88 +0.6 835,020 + BWI76_RS04035 0.88 -0.8 835,020 + BWI76_RS04035 0.88 -1.6 835,020 + BWI76_RS04035 0.88 -0.7 835,020 + BWI76_RS04035 0.88 -1.6 835,028 - BWI76_RS04035 0.89 -1.1 835,028 - BWI76_RS04035 0.89 +2.2 835,028 - BWI76_RS04035 0.89 -4.1 835,028 - BWI76_RS04035 0.89 -1.9 835,028 - BWI76_RS04035 0.89 +1.8 835,028 - BWI76_RS04035 0.89 -0.3 835,028 - BWI76_RS04035 0.89 -1.6 835,063 + -3.2 835,070 - +0.2 835,072 + +0.1 835,078 + +0.2 835,080 + +1.7 835,080 + -0.6 835,080 + -3.9 835,087 + +1.2 835,087 + +0.2 835,087 + -1.4 835,088 - +1.2 835,090 + -0.6 835,095 + +0.6 835,095 - -2.7 835,095 - +0.2 835,095 - -3.4 835,098 - -2.9 835,100 + -1.4 835,100 - -0.4 835,100 - -2.9 835,100 - -0.8 835,100 - -0.4 835,101 + -0.1 835,103 + -1.5 835,129 + +0.3 835,133 - -3.7 835,139 + +0.3 835,142 + -0.0 835,150 - +1.6 835,166 + BWI76_RS04040 0.11 -0.1 835,174 - BWI76_RS04040 0.13 -3.4 835,174 - BWI76_RS04040 0.13 +0.6 835,207 - BWI76_RS04040 0.20 +0.4 835,207 - BWI76_RS04040 0.20 +0.5 835,212 + BWI76_RS04040 0.21 -1.6 835,219 - BWI76_RS04040 0.23 -1.1 835,220 - BWI76_RS04040 0.23 +1.2 835,224 + BWI76_RS04040 0.24 +0.7 835,232 - BWI76_RS04040 0.26 +2.0 835,246 - BWI76_RS04040 0.29 +0.3 835,274 - BWI76_RS04040 0.35 +0.6 835,282 - BWI76_RS04040 0.37 +1.2 835,286 + BWI76_RS04040 0.37 +1.5 835,453 + BWI76_RS04040 0.74 +1.7 835,482 + BWI76_RS04040 0.80 +1.5 835,482 + BWI76_RS04040 0.80 -0.5 835,490 - BWI76_RS04040 0.82 -0.8 835,493 + BWI76_RS04040 0.83 -0.3 835,493 + BWI76_RS04040 0.83 -1.2 835,493 + BWI76_RS04040 0.83 -0.5 835,501 - BWI76_RS04040 0.84 +1.6 835,506 - BWI76_RS04040 0.85 +0.4 835,529 + -0.1 835,569 - -3.2 835,569 - +0.1 835,584 + +0.7 835,592 - -3.4 835,784 + +0.7 835,897 - +1.1 835,897 - -0.3 835,899 + -3.9 835,907 - -2.2 835,996 - +0.9 836,154 - BWI76_RS04045 0.18 +0.5 836,348 - BWI76_RS04045 0.32 -0.5 836,524 + BWI76_RS04045 0.45 +1.0 836,531 - BWI76_RS04045 0.46 +1.7 836,660 - BWI76_RS04045 0.56 -0.4 836,675 - BWI76_RS04045 0.57 -0.2 836,842 + BWI76_RS04045 0.69 +1.5 837,008 + BWI76_RS04045 0.81 +0.2 837,011 - BWI76_RS04045 0.82 +0.2 837,016 - BWI76_RS04045 0.82 -1.8 837,078 - BWI76_RS04045 0.87 +2.6 837,143 - +0.9 837,337 - -1.1 837,366 - -2.7 837,434 - BWI76_RS04050 0.16 -0.4 837,572 + BWI76_RS04050 0.34 -1.6 837,777 - +1.2 837,839 + BWI76_RS04050 0.68 -2.1 837,906 + BWI76_RS04050 0.76 -0.8 837,946 - BWI76_RS04050 0.81 -0.8 837,956 - BWI76_RS04050 0.83 +1.2 837,970 - BWI76_RS04050 0.84 +1.2 838,030 + -1.2 838,032 + -0.8 838,032 + -0.1 838,032 + -1.5 838,040 - -0.1
Or see this region's nucleotide sequence