Experiment: RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02355 and BWI76_RS02360 are separated by 134 nucleotides BWI76_RS02360 and BWI76_RS02365 are separated by 86 nucleotides BWI76_RS02365 and BWI76_RS02370 are separated by 23 nucleotides
BWI76_RS02355: BWI76_RS02355 - molecular chaperone GroEL, at 483,512 to 485,158
_RS02355
BWI76_RS02360: BWI76_RS02360 - hypothetical protein, at 485,293 to 485,646
_RS02360
BWI76_RS02365: BWI76_RS02365 - hypothetical protein, at 485,733 to 486,596
_RS02365
BWI76_RS02370: BWI76_RS02370 - colicin V synthesis protein, at 486,620 to 488,743
_RS02370
Position (kb)
485
486
487 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 485.083 kb on + strand at 485.174 kb on - strand at 485.259 kb on - strand at 485.329 kb on + strand, within BWI76_RS02360 at 485.329 kb on + strand, within BWI76_RS02360 at 485.342 kb on - strand, within BWI76_RS02360 at 485.342 kb on - strand, within BWI76_RS02360 at 485.414 kb on - strand, within BWI76_RS02360 at 485.435 kb on - strand, within BWI76_RS02360 at 485.472 kb on + strand, within BWI76_RS02360 at 485.480 kb on - strand, within BWI76_RS02360 at 485.517 kb on + strand, within BWI76_RS02360 at 485.550 kb on + strand, within BWI76_RS02360 at 485.591 kb on + strand, within BWI76_RS02360 at 485.591 kb on + strand, within BWI76_RS02360 at 485.599 kb on - strand, within BWI76_RS02360 at 485.599 kb on - strand, within BWI76_RS02360 at 485.625 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.634 kb on - strand at 485.634 kb on - strand at 485.634 kb on - strand at 485.634 kb on - strand at 485.645 kb on + strand at 485.656 kb on + strand at 485.656 kb on + strand at 485.657 kb on + strand at 485.662 kb on + strand at 485.662 kb on + strand at 485.664 kb on - strand at 485.665 kb on - strand at 485.669 kb on + strand at 485.670 kb on - strand at 485.672 kb on + strand at 485.680 kb on + strand at 485.683 kb on - strand at 485.712 kb on - strand at 485.738 kb on - strand at 485.738 kb on - strand at 485.771 kb on + strand at 485.788 kb on + strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.861 kb on - strand, within BWI76_RS02365 at 485.954 kb on + strand, within BWI76_RS02365 at 485.989 kb on + strand at 486.097 kb on + strand, within BWI76_RS02365 at 486.105 kb on - strand, within BWI76_RS02365 at 486.105 kb on - strand, within BWI76_RS02365 at 486.112 kb on - strand, within BWI76_RS02365 at 486.193 kb on + strand at 486.201 kb on - strand, within BWI76_RS02365 at 486.203 kb on + strand, within BWI76_RS02365 at 486.313 kb on - strand, within BWI76_RS02365 at 486.407 kb on + strand, within BWI76_RS02365 at 486.415 kb on - strand, within BWI76_RS02365 at 486.415 kb on - strand, within BWI76_RS02365 at 486.420 kb on - strand, within BWI76_RS02365 at 486.470 kb on + strand, within BWI76_RS02365 at 486.471 kb on + strand, within BWI76_RS02365 at 486.479 kb on - strand, within BWI76_RS02365 at 486.479 kb on - strand, within BWI76_RS02365 at 486.553 kb on + strand at 486.634 kb on + strand at 486.636 kb on - strand at 486.641 kb on - strand at 486.642 kb on - strand at 486.642 kb on - strand at 486.642 kb on - strand at 486.696 kb on - strand at 486.706 kb on + strand at 486.714 kb on - strand at 486.724 kb on - strand at 486.865 kb on - strand, within BWI76_RS02370 at 487.038 kb on + strand, within BWI76_RS02370 at 487.038 kb on + strand, within BWI76_RS02370 at 487.093 kb on + strand, within BWI76_RS02370 at 487.093 kb on + strand, within BWI76_RS02370 at 487.101 kb on - strand, within BWI76_RS02370 at 487.104 kb on - strand, within BWI76_RS02370 at 487.154 kb on - strand, within BWI76_RS02370 at 487.154 kb on - strand, within BWI76_RS02370 at 487.154 kb on - strand, within BWI76_RS02370 at 487.156 kb on + strand, within BWI76_RS02370 at 487.164 kb on - strand, within BWI76_RS02370 at 487.194 kb on - strand, within BWI76_RS02370 at 487.286 kb on - strand, within BWI76_RS02370 at 487.321 kb on - strand at 487.321 kb on - strand, within BWI76_RS02370 at 487.321 kb on - strand at 487.407 kb on - strand, within BWI76_RS02370 at 487.451 kb on - strand, within BWI76_RS02370 at 487.462 kb on - strand, within BWI76_RS02370 at 487.468 kb on - strand, within BWI76_RS02370 at 487.550 kb on - strand, within BWI76_RS02370 at 487.564 kb on - strand, within BWI76_RS02370 at 487.578 kb on - strand, within BWI76_RS02370
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x remove 485,083 + -2.8 485,174 - -0.7 485,259 - -0.7 485,329 + BWI76_RS02360 0.10 -0.5 485,329 + BWI76_RS02360 0.10 -1.2 485,342 - BWI76_RS02360 0.14 -4.2 485,342 - BWI76_RS02360 0.14 -2.3 485,414 - BWI76_RS02360 0.34 -1.9 485,435 - BWI76_RS02360 0.40 +1.0 485,472 + BWI76_RS02360 0.51 +0.8 485,480 - BWI76_RS02360 0.53 +0.3 485,517 + BWI76_RS02360 0.63 +0.3 485,550 + BWI76_RS02360 0.73 +1.3 485,591 + BWI76_RS02360 0.84 +0.5 485,591 + BWI76_RS02360 0.84 -2.0 485,599 - BWI76_RS02360 0.86 +0.8 485,599 - BWI76_RS02360 0.86 -1.7 485,625 + -2.5 485,626 + +0.0 485,626 + +0.2 485,626 + -1.3 485,626 + -1.3 485,626 + -0.2 485,626 + +1.3 485,626 + -0.5 485,626 + +0.0 485,626 + +1.0 485,634 - +1.8 485,634 - +1.3 485,634 - -1.5 485,634 - -1.5 485,645 + -0.8 485,656 + -0.3 485,656 + +1.5 485,657 + -1.9 485,662 + +0.4 485,662 + -0.3 485,664 - -2.2 485,665 - +1.6 485,669 + -1.7 485,670 - +1.2 485,672 + +0.4 485,680 + +0.1 485,683 - +0.7 485,712 - +0.6 485,738 - -4.0 485,738 - +0.5 485,771 + -0.3 485,788 + -1.7 485,796 - +1.9 485,796 - -3.0 485,796 - +0.9 485,796 - -2.7 485,796 - +0.1 485,861 - BWI76_RS02365 0.15 +1.3 485,954 + BWI76_RS02365 0.26 -1.3 485,989 + +1.3 486,097 + BWI76_RS02365 0.42 -3.2 486,105 - BWI76_RS02365 0.43 -1.9 486,105 - BWI76_RS02365 0.43 -1.7 486,112 - BWI76_RS02365 0.44 -3.0 486,193 + +1.3 486,201 - BWI76_RS02365 0.54 +0.3 486,203 + BWI76_RS02365 0.54 -1.7 486,313 - BWI76_RS02365 0.67 -0.3 486,407 + BWI76_RS02365 0.78 -1.0 486,415 - BWI76_RS02365 0.79 +1.2 486,415 - BWI76_RS02365 0.79 -2.7 486,420 - BWI76_RS02365 0.80 +1.3 486,470 + BWI76_RS02365 0.85 +5.5 486,471 + BWI76_RS02365 0.85 -3.2 486,479 - BWI76_RS02365 0.86 -2.7 486,479 - BWI76_RS02365 0.86 +1.0 486,553 + -0.3 486,634 + -0.3 486,636 - +1.3 486,641 - +0.3 486,642 - -1.5 486,642 - -2.0 486,642 - +0.9 486,696 - +0.5 486,706 + +0.3 486,714 - -1.7 486,724 - -0.7 486,865 - BWI76_RS02370 0.12 +0.3 487,038 + BWI76_RS02370 0.20 -1.5 487,038 + BWI76_RS02370 0.20 +1.7 487,093 + BWI76_RS02370 0.22 +1.3 487,093 + BWI76_RS02370 0.22 -2.3 487,101 - BWI76_RS02370 0.23 +0.3 487,104 - BWI76_RS02370 0.23 +1.3 487,154 - BWI76_RS02370 0.25 +0.1 487,154 - BWI76_RS02370 0.25 -1.0 487,154 - BWI76_RS02370 0.25 +0.3 487,156 + BWI76_RS02370 0.25 -1.7 487,164 - BWI76_RS02370 0.26 -0.3 487,194 - BWI76_RS02370 0.27 -1.7 487,286 - BWI76_RS02370 0.31 -2.6 487,321 - -0.3 487,321 - BWI76_RS02370 0.33 -1.9 487,321 - +1.3 487,407 - BWI76_RS02370 0.37 -1.0 487,451 - BWI76_RS02370 0.39 +1.3 487,462 - BWI76_RS02370 0.40 -0.3 487,468 - BWI76_RS02370 0.40 -0.3 487,550 - BWI76_RS02370 0.44 -3.2 487,564 - BWI76_RS02370 0.44 +0.3 487,578 - BWI76_RS02370 0.45 +0.6
Or see this region's nucleotide sequence