Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26345 and BPHYT_RS26350 are separated by 155 nucleotides BPHYT_RS26350 and BPHYT_RS26355 are separated by 51 nucleotides BPHYT_RS26355 and BPHYT_RS26360 are separated by 199 nucleotides BPHYT_RS26360 and BPHYT_RS26365 are separated by 160 nucleotides
BPHYT_RS26345: BPHYT_RS26345 - hypothetical protein, at 1,484,597 to 1,484,857
_RS26345
BPHYT_RS26350: BPHYT_RS26350 - hypothetical protein, at 1,485,013 to 1,485,249
_RS26350
BPHYT_RS26355: BPHYT_RS26355 - hypothetical protein, at 1,485,301 to 1,485,546
_RS26355
BPHYT_RS26360: BPHYT_RS26360 - hypothetical protein, at 1,485,746 to 1,485,943
_RS26360
BPHYT_RS26365: BPHYT_RS26365 - oxidoreductase molybdopterin subunit, at 1,486,104 to 1,488,470
_RS26365
Position (kb) 1485
1486 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1484.302 kb on + strand at 1484.303 kb on - strand at 1484.773 kb on - strand, within BPHYT_RS26345 at 1485.248 kb on - strand at 1485.467 kb on + strand, within BPHYT_RS26355 at 1485.492 kb on - strand, within BPHYT_RS26355
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB remove 1,484,302 + -3.5 1,484,303 - +0.7 1,484,773 - BPHYT_RS26345 0.67 -1.1 1,485,248 - -0.3 1,485,467 + BPHYT_RS26355 0.67 +1.3 1,485,492 - BPHYT_RS26355 0.78 -0.8
Or see this region's nucleotide sequence